| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 832,097 | T→C | 22.6% | R185R (CGT→CGC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 832,097 | 0 | T | C | 22.6% | 154.6 / 42.9 | 84 | R185R (CGT→CGC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (36/29); new base C (13/6); total (49/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
AGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCG > Exported/832036‑832166 | aggTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGt < 1:215467/71‑1 (MQ=255) gaacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTtct > 1:4393584/1‑71 (MQ=255) gaacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTtct > 1:493275/1‑71 (MQ=255) gaacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTtct > 1:4941474/1‑71 (MQ=255) aacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAATTACGGCCCGGACCGTGTCGCTGGCTtctc > 1:2628453/1‑71 (MQ=255) aacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAATTACGGCCCGGACCGTGTCGCTGGCTtctc > 1:4040059/1‑71 (MQ=255) aacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTtct < 1:131151/70‑1 (MQ=255) aacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTtc < 1:5481344/69‑1 (MQ=255) cgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGc > 1:3485223/1‑71 (MQ=255) gaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGcc < 1:4976961/71‑1 (MQ=255) cTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAAt < 1:4964170/71‑1 (MQ=255) attgccgccGCTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATccc < 1:3152349/68‑1 (MQ=255) aTCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCgtgt < 1:4243162/50‑1 (MQ=255) aTCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTcc > 1:936587/1‑71 (MQ=255) tCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCgtg < 1:4168738/48‑1 (MQ=255) tCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAAt > 1:1866958/1‑67 (MQ=255) tCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCg > 1:2305049/1‑71 (MQ=255) cgccgcATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTg > 1:3150246/1‑50 (MQ=255) gccgccGCTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGc > 1:634764/1‑71 (MQ=255) gccgccGCTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGc > 1:4053174/1‑71 (MQ=255) gccgcATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTCGCTGGTTTCTCGCCAATTCCGGc < 1:2273519/71‑1 (MQ=255) cgcATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCaa > 1:123709/1‑71 (MQ=255) gcATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCgtg < 1:3474836/43‑1 (MQ=255) cATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGtt > 1:2335341/1‑51 (MQ=255) cATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATg < 1:5015116/71‑1 (MQ=255) tCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTc < 1:4360545/61‑1 (MQ=255) tCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGc < 1:2610061/65‑1 (MQ=255) tCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGc < 1:993397/65‑1 (MQ=255) tCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTc > 1:4559810/1‑71 (MQ=255) gcTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGc < 1:183876/64‑1 (MQ=255) gcTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGTc < 1:5170591/70‑1 (MQ=255) tAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTcc > 1:5432170/1‑60 (MQ=255) tAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGc < 1:2670855/63‑1 (MQ=255) tAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGtt < 1:2404767/47‑1 (MQ=255) tAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTtct > 1:5250444/1‑50 (MQ=255) aaCGTTTACACCATCAAAAACTACGGCCCGGACCgtgt > 1:1888533/1‑38 (MQ=255) aaCGTTTACACCATCAAAAACTACGGCCCGGACCgtgt > 1:1158981/1‑38 (MQ=255) aaCGTTTACACCATCAAAAACTACGGCCCGGACCgtg > 1:3044162/1‑37 (MQ=255) aaCGTTTACACCATCAAAAACTACGGCCCGGACCgtg > 1:1733049/1‑37 (MQ=255) aaCGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCg > 1:517407/1‑69 (MQ=255) aaCGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGAt > 1:317158/1‑71 (MQ=255) aCGTTTACACCATCAAAAACTACGGCCCGGACCgtgt < 1:2798108/37‑1 (MQ=255) aCGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCg < 1:3344827/59‑1 (MQ=255) aCGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCaa > 1:2961017/1‑63 (MQ=255) aCGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTc < 1:2022767/67‑1 (MQ=255) aCGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTc < 1:3704031/67‑1 (MQ=255) cGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTtctc > 1:2013047/1‑48 (MQ=255) gTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGt > 1:1435766/1‑71 (MQ=255) tttACACCATCAAAAACTACGGCCCGGACCGTGTTGc < 1:1025657/37‑1 (MQ=255) tttACACCATCAAAAACTACGGCCCGGACCGTGTTGCt < 1:5180480/38‑1 (MQ=255) tttACACCATCAAAAACTACGGCCCGGACCGTGTTGCt < 1:3931721/38‑1 (MQ=255) tttACACCATCAAAAACTACGGCCCGGACCGTGTTGCt < 1:3938579/38‑1 (MQ=255) ttACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGtt > 1:3071241/1‑70 (MQ=255) tACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATg > 1:3673520/1‑60 (MQ=255) tACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGttt < 1:3578637/70‑1 (MQ=255) tACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGttt < 1:2123143/70‑1 (MQ=255) gacCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGTCGATGGTTTCt > 1:1141376/2‑70 (MQ=255) cacCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCtt < 1:1033628/71‑1 (MQ=255) cacCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCt < 1:3327177/70‑1 (MQ=255) cATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAAt > 1:4634210/1‑45 (MQ=255) cATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCtt > 1:15918/1‑68 (MQ=255) cATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTa > 1:64348/1‑69 (MQ=255) cATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCAGGCAATGTCGATGGTTTCTTACg > 1:89437/1‑71 (MQ=255) aTCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGTCGATgg < 1:3825966/61‑1 (MQ=255) aTCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGTCGATGGTTTc < 1:4413210/65‑1 (MQ=255) tCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGTCGATGGTTTCTTACGcc > 1:4959062/1‑70 (MQ=255) tCAAAACCTACGGCCCGGATCGCGATGCCGGTTtc > 1:2449897/1‑35 (MQ=12) tCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTc < 1:4729009/45‑1 (MQ=255) tCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCa < 1:4060976/71‑1 (MQ=255) tCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCa > 1:4398508/1‑71 (MQ=255) tCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCa > 1:687530/1‑71 (MQ=255) cAAAACCTACGGCCCGGATCGCGTTGCCGGTTtct > 1:2846002/1‑35 (MQ=19) cAAAACCTACGGCCCGGATCGCGTTGCCGGTTtct > 1:1182619/1‑35 (MQ=19) cAAAACCTACGGCCCGGATCGCGTTGCCGGTTtct > 1:3613328/1‑35 (MQ=19) aaaacCTACGGCCCGGATCGCGTTGCCGGTATCTCGCCGATCCCGGCGATGTCGATGGTTTCTTACGccgc > 1:5204152/1‑68 (MQ=255) aaaaaCTACGGCCCGGACCGTGTTGCTGGTTtctc > 1:1357552/1‑35 (MQ=255) aaaaaCTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTa > 1:4121883/1‑65 (MQ=255) aaaaaCTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTa > 1:4345175/1‑65 (MQ=255) aaaaaCTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTa > 1:911313/1‑65 (MQ=255) aaaaaCTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCAt < 1:2540267/70‑1 (MQ=255) aaacCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGcga > 1:12496/1‑46 (MQ=255) aaacCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGTCGa > 1:5329077/1‑54 (MQ=255) aaaaCTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATgg > 1:105675/1‑57 (MQ=255) aaaaCTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATgg > 1:4079964/1‑57 (MQ=255) aaaCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCgg > 1:141378/1‑44 (MQ=25) cccGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACg > 1:3754798/1‑69 (MQ=255) ccGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCt > 1:1641594/1‑70 (MQ=255) gACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCa < 1:4593028/54‑1 (MQ=255) ccGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTAtctct > 1:3760901/1‑71 (MQ=255) cGTGTTGCTGGTTTCTCTCCAATTCCGGCAATGTCGATGGTTTCt < 1:5041481/45‑1 (MQ=255) cGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCt < 1:4416514/45‑1 (MQ=255) cGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTAtctctc < 1:432230/71‑1 (MQ=255) tgtTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTa > 1:2281001/1‑45 (MQ=255) tgtTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTa > 1:4226510/1‑45 (MQ=255) tgtTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCg > 1:5561017/1‑70 (MQ=255) tgtTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCg > 1:4606854/1‑70 (MQ=255) | AGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTTACGCATCGGGTGCACGCTATCTCTCG > Exported/832036‑832166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |