| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 832,313 | G→A | 41.2% | V257V (GTG→GTA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 832,313 | 0 | G | A | 41.2% | 65.4 / 123.5 | 114 | V257V (GTG→GTA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (37/30); new base A (22/25); total (59/55) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
GCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCG > Exported/832244‑832383 | gCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGc < 1:383673/71‑1 (MQ=255) aaCTGACGTACTGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCg > 1:2519185/1‑70 (MQ=255) aaCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCg > 1:3401781/1‑70 (MQ=255) accgACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCg > 1:468365/4‑69 (MQ=255) accgACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCa < 1:5522420/68‑1 (MQ=255) aCTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCa < 1:2961473/71‑1 (MQ=255) gACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGa > 1:781607/1‑70 (MQ=255) gACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGa > 1:1956245/1‑70 (MQ=255) gACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGa > 1:5269461/1‑70 (MQ=255) gACGTACCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGAc < 1:4100523/71‑1 (MQ=255) aCGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGAcg > 1:2852621/1‑71 (MQ=255) aCCGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCg < 1:3213199/61‑1 (MQ=255) ccGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTAc > 1:2828340/1‑71 (MQ=255) cGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGAcgcg < 1:3832576/67‑1 (MQ=255) cGGAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCg < 1:1970589/59‑1 (MQ=38) ggAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCg < 1:4413453/58‑1 (MQ=38) gAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAcccc > 1:676253/1‑71 (MQ=255) gAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAcccc > 1:2135068/1‑71 (MQ=255) gAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAcc > 1:3576965/1‑69 (MQ=255) gAATCTGCCGACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTAcgcc < 1:878330/71‑1 (MQ=255) aaTCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGt < 1:2089654/65‑1 (MQ=255) aTCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCgg < 1:3040704/71‑1 (MQ=255) tCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAc > 1:166102/1‑65 (MQ=255) tCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAc > 1:127842/1‑65 (MQ=255) tCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAc > 1:240491/1‑65 (MQ=255) ctgctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGAt > 1:2694472/1‑71 (MQ=255) tgctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATg > 1:4384016/1‑71 (MQ=255) tgccgACTGGTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGacg > 1:3068280/1‑69 (MQ=255) gctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGAt > 1:1039279/1‑69 (MQ=255) gctgACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCACGGAt > 1:2394206/1‑69 (MQ=255) ggTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCg > 1:1345933/1‑44 (MQ=255) gTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCActtct > 1:3538926/1‑71 (MQ=255) gTATAACTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCActtc < 1:3426758/70‑1 (MQ=255) gTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActt < 1:1521/69‑1 (MQ=255) tACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAcccc < 1:5281022/56‑1 (MQ=255) tACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTAcccc < 1:853902/56‑1 (MQ=255) tACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:2389662/71‑1 (MQ=255) aCAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtctt > 1:5534822/1‑70 (MQ=255) taaCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCttt < 1:1688819/69‑1 (MQ=255) taaCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCttt < 1:4595201/69‑1 (MQ=255) taaCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTa > 1:5046981/2‑71 (MQ=255) aaCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAg < 1:2017198/42‑1 (MQ=255) aaCTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCt < 1:2662608/60‑1 (MQ=255) aaCTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCt < 1:4730690/60‑1 (MQ=255) aCTCCAGCTACATCATCGCCTGGGGGTCTAACGTACCTCAGACACGTACGCCGGACGCCCACTTCTTTAcc > 1:3817375/1‑70 (MQ=255) cTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGc > 1:2948096/1‑38 (MQ=255) tCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCActt < 1:1938902/62‑1 (MQ=255) tCCAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTAccg < 1:2584830/70‑3 (MQ=255) ccAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGa > 1:3316330/1‑52 (MQ=255) ccAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGAt > 1:1726886/1‑53 (MQ=255) cAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGa < 1:4799217/69‑1 (MQ=255) cAGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGa < 1:650272/69‑1 (MQ=255) aGCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGt < 1:4962118/71‑1 (MQ=255) gCTACATCATCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCg < 1:2546545/47‑1 (MQ=255) cctcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGt > 1:3551809/3‑70 (MQ=255) catcatCGCCTGGGGTTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtc > 1:4994262/1‑55 (MQ=255) catcatCGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACgc < 1:4611402/70‑2 (MQ=255) catcatCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGt > 1:2484509/1‑70 (MQ=255) atcatcGCCTGGGGGTCTATCGTACCGCAGACACGTACGCCGGACGCCCActt < 1:134433/53‑1 (MQ=255) tcatcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTa > 1:2728192/1‑57 (MQ=255) catcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTAc > 1:5535609/1‑70 (MQ=255) catcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTAc > 1:4222716/1‑70 (MQ=255) atcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACa > 1:4172383/1‑70 (MQ=255) atcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACaa < 1:2475442/71‑1 (MQ=255) atcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACa > 1:1620070/1‑70 (MQ=255) atcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACa > 1:2794444/1‑70 (MQ=255) tcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTAccg < 1:812926/57‑3 (MQ=255) tcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTAccg < 1:3721680/57‑3 (MQ=255) tcGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACAGAAGTACGCTACaaa > 1:2735956/1‑71 (MQ=255) tcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCActtc < 1:2392652/50‑1 (MQ=255) tcGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACaaa < 1:3325938/71‑1 (MQ=255) cGCCTGGGGGTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAg > 1:1353362/1‑71 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAg < 1:5040542/70‑1 (MQ=255) gCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAg < 1:2757397/70‑1 (MQ=255) ggggTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCActa < 1:2941623/71‑3 (MQ=255) ggggTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCActa < 1:3623406/71‑3 (MQ=255) gggTCTAACGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTaa < 1:5529506/71‑1 (MQ=255) gggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaa < 1:3847510/71‑1 (MQ=255) gggTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaa < 1:1378252/71‑1 (MQ=255) tctaaCGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAAc < 1:5613126/68‑1 (MQ=255) cAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg > 1:113967/1‑61 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:5332486/39‑1 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:3573448/39‑1 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGaa > 1:1881890/1‑46 (MQ=255) aaaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAgg < 1:3776140/60‑1 (MQ=255) aaCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCaaa < 1:1482629/66‑1 (MQ=255) aCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGtc < 1:3143133/71‑2 (MQ=255) aCGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGt < 1:164303/70‑1 (MQ=255) aCGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAg > 1:464371/1‑45 (MQ=255) aCGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAg > 1:2466735/1‑45 (MQ=255) aCGTACCGCAGACACGTACGCCGGACGCCCACTTCTTTACCGAAGTACGCTACAAAGGCACTAAAAc > 1:1477693/1‑67 (MQ=255) cGTGCCGCAGACGCGTACCCCGGATGCTCActtctt < 1:3762885/36‑1 (MQ=255) cGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAAc > 1:270907/1‑66 (MQ=255) cGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTg > 1:824326/1‑71 (MQ=255) gTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTg > 1:2942999/1‑70 (MQ=255) gTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGc > 1:654801/1‑71 (MQ=255) gTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGc > 1:2328699/1‑71 (MQ=255) tGCCGCAGACGCGTACCCCGGATGCTCACTTCtttt > 1:3518208/1‑35 (MQ=255) tGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTa > 1:4410367/1‑36 (MQ=255) tGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGcc > 1:350784/1‑71 (MQ=255) | GCAAACTGACGTACCGGAATCTGCTGACTGGTACAACTCCAGCTACATCATCGCCTGGGGGTCAAACGTGCCGCAGACGCGTACCCCGGATGCTCACTTCTTTACTGAAGTGCGTTACAAAGGGACCAAAACTGTTGCCG > Exported/832244‑832383 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |