Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 833,174 G→T 17.9% G544G (GGG→GGT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported833,1740GT17.9% 203.4 / 24.5 78G544G (GGG→GGTnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (33/31);  new base T (11/3);  total (44/34)
Fisher's exact test for biased strand distribution p-value = 7.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

TCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTG  >  Exported/833107‑833244
                                                                   |                                                                      
tCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCt                                                                      >  1:5553360/1‑70 (MQ=255)
  gCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGa                                                                    <  1:4305386/70‑1 (MQ=255)
   cccGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAAc                                                                  <  1:1926014/71‑1 (MQ=255)
      gTGAATTTGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCAc                                                               <  1:3346124/71‑1 (MQ=255)
        gAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTg                                                                     <  1:725909/63‑1 (MQ=255)
        gAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTg                                                                     <  1:2100598/63‑1 (MQ=255)
        gAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTg                                                             >  1:3616516/1‑71 (MQ=255)
         aaTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTg                                                                     <  1:3599317/62‑1 (MQ=255)
         aaTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTgg                                                            >  1:3454848/1‑71 (MQ=255)
          aTTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGt                                                           <  1:5615322/71‑1 (MQ=255)
             tggcGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTAt                                                         >  1:5308718/4‑70 (MQ=255)
             tGCCGATACCGCCCATAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCa                                                                >  1:3023558/1‑63 (MQ=255)
             tGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTAt                                                         >  1:2204781/1‑70 (MQ=255)
                  aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                                   >  1:3836548/1‑71 (MQ=255)
                  aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                                   >  1:511904/1‑71 (MQ=255)
                  aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                                   >  1:2843909/1‑71 (MQ=255)
                  aTAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                                   >  1:5594759/1‑71 (MQ=255)
                    aaCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                                   <  1:3817943/69‑1 (MQ=255)
                    aaCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                                   <  1:3811269/69‑1 (MQ=255)
                    aaCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTc                                                   <  1:4694658/69‑1 (MQ=255)
                    aaCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGa                                                 >  1:3457660/1‑71 (MQ=255)
                     aCGCTGATAAAACGCACGGTCGTTCGATGATTATCGTAGGTGGGGGGCTGAACCACTGGTATCACCTc                                                   <  1:5588011/68‑1 (MQ=255)
                      cGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCg                                                  >  1:3274504/1‑68 (MQ=255)
                          caTAAAACGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGaa                                           >  1:5055733/2‑71 (MQ=255)
                           aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGa                                            <  1:3246245/69‑1 (MQ=255)
                           aTAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGa                                            <  1:988636/69‑1 (MQ=255)
                           aTAAAACGCACGGTCGTTCGATGATTATCGTAGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAAc                                          >  1:652935/1‑71 (MQ=255)
                             aaaaCGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTa                                        >  1:5524510/1‑71 (MQ=255)
                             aaaaCGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTa                                        >  1:5373823/1‑71 (MQ=255)
                             aaaaCGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTa                                        >  1:2336230/1‑71 (MQ=255)
                             aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGGTGAACCACt                                                              >  1:3204065/1‑49 (MQ=255)
                             aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACt                                         >  1:114290/1‑70 (MQ=255)
                             aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTa                                        >  1:4628471/1‑71 (MQ=255)
                             aaaaCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTa                                        >  1:3878286/1‑71 (MQ=255)
                              aaaCGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCAc                                                      >  1:5116378/1‑56 (MQ=255)
                              aaaCGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCAc                                                      >  1:1484765/1‑56 (MQ=255)
                              aaaCGCATGGGCGCTCGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCAc                                                      >  1:1708728/1‑56 (MQ=255)
                                aCGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg                                     >  1:1316998/1‑71 (MQ=255)
                                 cGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGt                                    >  1:669525/1‑71 (MQ=255)
                                   cACGGTCGTTCGATGATTATCGTCGGTGCGCGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                                  <  1:1004468/71‑1 (MQ=255)
                                    aCGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGt                                 <  1:3815336/71‑1 (MQ=255)
                                       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACt                                                              <  1:3155028/39‑1 (MQ=255)
                                       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                <  1:611356/69‑1 (MQ=255)
                                       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                <  1:5667402/69‑1 (MQ=255)
                                       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                <  1:349528/69‑1 (MQ=255)
                                       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                <  1:1454271/69‑1 (MQ=255)
                                       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCt                               >  1:1401083/1‑70 (MQ=255)
                                       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                              <  1:5611212/71‑1 (MQ=255)
                                       gTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTg                              <  1:1420865/71‑1 (MQ=255)
                                        tCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGa                             >  1:5689090/1‑71 (MQ=255)
                                        tCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGa                             >  1:4611107/1‑71 (MQ=255)
                                          gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCg                                     >  1:2467066/1‑61 (MQ=255)
                                          gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGAt                            >  1:1571150/1‑70 (MQ=255)
                                          gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATc                           <  1:2996689/71‑1 (MQ=255)
                                          gTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATc                           <  1:2989894/71‑1 (MQ=255)
                                           ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGa                                                 <  1:588702/48‑1 (MQ=255)
                                           ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGa                                                 <  1:5588474/48‑1 (MQ=255)
                                           ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                          <  1:5547149/71‑1 (MQ=255)
                                           ttCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                          <  1:1583912/71‑1 (MQ=255)
                                             cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                         >  1:1259031/1‑70 (MQ=255)
                                             cGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                         >  1:5430147/1‑70 (MQ=255)
                                             cGATGATTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaa                         >  1:2968102/1‑70 (MQ=255)
                                              gatgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCaa                         <  1:5499255/69‑1 (MQ=255)
                                               atgatTATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAatat                      <  1:4954499/71‑1 (MQ=255)
                                                tgatTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCaa                         <  1:5172200/67‑1 (MQ=255)
                                                  atTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGc                    <  1:3981034/70‑1 (MQ=255)
                                                   ttATCCTCGGCGCAGGTGTTAACCACTGGTATCACATGGACATGAACTACCGCGGGATGATCAATATGCt                   >  1:4704277/1‑70 (MQ=255)
                                                      tcgtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATc                           >  1:1731914/1‑59 (MQ=255)
                                                      tcgtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCa                          >  1:5692257/1‑60 (MQ=255)
                                                      tcgtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAtt               >  1:5324934/1‑71 (MQ=255)
                                                      tcgtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAt                <  1:4684714/70‑1 (MQ=255)
                                                        gtcgGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTc                                <  1:3251542/52‑1 (MQ=255)
                                                              gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTgg                                  >  1:4609535/1‑44 (MQ=255)
                                                              gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAtttt             >  1:4236686/1‑65 (MQ=255)
                                                              gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGAtttt             >  1:2960422/1‑65 (MQ=255)
                                                              gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCgg       >  1:5051978/1‑71 (MQ=255)
                                                              gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCg        >  1:4316618/1‑70 (MQ=255)
                                                              gCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCg        >  1:5672695/1‑70 (MQ=255)
                                                                 gggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTg          <  1:5569653/65‑1 (MQ=255)
                                                                 gggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTg          <  1:1363383/65‑1 (MQ=255)
                                                                  ggCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgt   <  1:1035629/71‑1 (MQ=255)
                                                                   gCTGAACCACTGGTATGACCTCGATATGAACTATCGTGGTc                                <  1:1450204/41‑1 (MQ=255)
                                                                   gCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCtgtg  >  1:3725412/1‑71 (MQ=255)
                                                                   |                                                                      
TCGCCCGTGAATTTGCCGATAACGCTGATAAAACGCACGGTCGTTCGATGATTATCGTCGGTGCGGGGCTGAACCACTGGTATCACCTCGATATGAACTATCGTGGTCTGATCAACATGCTGATTTTCTGCGGCTGTG  >  Exported/833107‑833244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: