Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 833,349 G→C 19.5% A603P (GCG→CCG)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported833,3490GC19.5% 104.7 / 18.5 51A603P (GCG→CCG) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (25/16);  new base C (5/5);  total (30/21)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGG  >  Exported/833286‑833418
                                                               |                                                                     
gAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGgc                                                                      <  1:3114799/65‑1 (MQ=255)
  aaaaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTcaca                                                              >  1:2860713/1‑71 (MQ=255)
    aaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg                                                            <  1:3706132/71‑1 (MQ=255)
    aaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg                                                            <  1:3900721/71‑1 (MQ=255)
    aaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg                                                            <  1:5646108/71‑1 (MQ=255)
    aaCTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATg                                                            <  1:1513747/71‑1 (MQ=255)
          gTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAg                                                       >  1:2884735/1‑70 (MQ=255)
           tCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTca                                                                <  1:2669784/60‑1 (MQ=255)
           tCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCa                                                     >  1:327492/1‑71 (MQ=255)
             cacAAACCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATGAAcaccac                                                    >  1:657669/3‑70 (MQ=255)
             cGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCAct                                                   >  1:2537324/1‑71 (MQ=255)
              acAAACCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCAcc                                                   >  1:5181827/2‑69 (MQ=255)
                aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg                                                            <  1:1340339/59‑1 (MQ=255)
                aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCActtct                                                <  1:5119343/71‑1 (MQ=255)
                aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCActtct                                                <  1:5112745/71‑1 (MQ=255)
                aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCActtct                                                <  1:1205984/71‑1 (MQ=255)
                  aCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGaa                                                          >  1:1047678/1‑59 (MQ=255)
                  aCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTa                                              >  1:697915/1‑71 (MQ=255)
                      gCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTc                                          >  1:4419780/1‑71 (MQ=255)
                      gCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTc                                          >  1:17889/1‑71 (MQ=255)
                              ccGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGTACAGc                                                      <  1:3527937/51‑1 (MQ=255)
                                gCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCa                                                     >  1:209608/1‑50 (MQ=255)
                                gCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCAc                                                    >  1:2019425/1‑51 (MQ=255)
                                gCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCAc                                                    >  1:3417849/1‑51 (MQ=255)
                                     cGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagc                           <  1:1808947/71‑1 (MQ=255)
                                      gTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagcc                          <  1:5274879/71‑1 (MQ=255)
                                      ctttGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagcc                          <  1:3783453/70‑1 (MQ=255)
                                       tttGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCa                                  <  1:1780901/62‑1 (MQ=255)
                                       tttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGaa                                                          >  1:5567355/1‑38 (MQ=255)
                                       tttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGaa                                                          >  1:4972207/1‑38 (MQ=255)
                                        ttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagccag                        >  1:926560/1‑71 (MQ=255)
                                        ttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagccag                        >  1:3296858/1‑71 (MQ=255)
                                         tGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagcc                          <  1:1474575/68‑1 (MQ=255)
                                         tGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagcc                          <  1:2288136/68‑1 (MQ=255)
                                         tGCCATTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTAt                                             >  1:1832980/1‑49 (MQ=255)
                                            ccTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGc                    <  1:1856097/71‑1 (MQ=255)
                                            ccTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGc                    >  1:4594606/1‑71 (MQ=255)
                                              ttGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGt                  >  1:4556130/1‑71 (MQ=255)
                                               tGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGt                  >  1:3218542/1‑70 (MQ=255)
                                                gACTGGCAGCGTCCGGCTCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagcca                         >  1:2850527/1‑62 (MQ=255)
                                                gACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCa                                  >  1:436715/1‑53 (MQ=255)
                                                gACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagccaa                        >  1:3442579/1‑62 (MQ=255)
                                                gACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTCCAGCCAATGGCGCTa                >  1:1517193/1‑71 (MQ=255)
                                                  cTGGCAGCGTCCGGCGCGTCCCATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTAt               >  1:706975/1‑70 (MQ=255)
                                                     gCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaaa           <  1:4814578/71‑1 (MQ=255)
                                                     gCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATATCTATAACCa                                  >  1:2405054/1‑48 (MQ=255)
                                                      cAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAc          >  1:416713/1‑71 (MQ=255)
                                                      cAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAc          >  1:674545/1‑71 (MQ=255)
                                                       aGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAcc         <  1:3192181/71‑1 (MQ=255)
                                                           tCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaa            <  1:3531251/64‑1 (MQ=255)
                                                           tCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaa            <  1:1168531/64‑1 (MQ=255)
                                                             cGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTa                >  1:4357240/1‑58 (MQ=255)
                                                              ggCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcgg  >  1:114987/1‑71 (MQ=255)
                                                               |                                                                     
GAAAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGG  >  Exported/833286‑833418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: