Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 833,354 T→C 19.6% R604R (CGT→CGC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported833,3540TC19.6% 110.7 / 20.4 56R604R (CGT→CGCnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (27/18);  new base C (5/6);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAG  >  Exported/833288‑833424
                                                                  |                                                                      
aaaaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTcaca                                                                    >  1:2860713/1‑71 (MQ=255)
  aaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg                                                                  <  1:3706132/71‑1 (MQ=255)
  aaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg                                                                  <  1:5646108/71‑1 (MQ=255)
  aaCTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg                                                                  <  1:3900721/71‑1 (MQ=255)
  aaCTGCGCCCACAAACCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATg                                                                  <  1:1513747/71‑1 (MQ=255)
        gTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAg                                                             >  1:2884735/1‑70 (MQ=255)
         tCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTca                                                                      <  1:2669784/60‑1 (MQ=255)
         tCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCa                                                           >  1:327492/1‑71 (MQ=255)
           cacAAACCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATGAAcaccac                                                          >  1:657669/3‑70 (MQ=255)
           cGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCAct                                                         >  1:2537324/1‑71 (MQ=255)
            acAAACCGGCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCAcc                                                         >  1:5181827/2‑69 (MQ=255)
              aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATg                                                                  <  1:1340339/59‑1 (MQ=255)
              aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCActtct                                                      <  1:1205984/71‑1 (MQ=255)
              aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCActtct                                                      <  1:5112745/71‑1 (MQ=255)
              aaaCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCActtct                                                      <  1:5119343/71‑1 (MQ=255)
                aCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGaa                                                                >  1:1047678/1‑59 (MQ=255)
                aCCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTa                                                    >  1:697915/1‑71 (MQ=255)
                    gCTGGTTGCCACTGGCCTTTGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTc                                                >  1:4419780/1‑71 (MQ=255)
                    gCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTc                                                >  1:17889/1‑71 (MQ=255)
                            ccGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGTACAGc                                                            <  1:3527937/51‑1 (MQ=255)
                              gCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCa                                                           >  1:209608/1‑50 (MQ=255)
                              gCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCAc                                                          >  1:2019425/1‑51 (MQ=255)
                              gCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCAc                                                          >  1:3417849/1‑51 (MQ=255)
                                   cGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagc                                 <  1:1808947/71‑1 (MQ=255)
                                    gTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagcc                                <  1:5274879/71‑1 (MQ=255)
                                    ctttGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagcc                                <  1:3783453/70‑1 (MQ=255)
                                     tttGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCa                                        <  1:1780901/62‑1 (MQ=255)
                                     tttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGaa                                                                >  1:4972207/1‑38 (MQ=255)
                                     tttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGaa                                                                >  1:5567355/1‑38 (MQ=255)
                                      ttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagccag                              >  1:926560/1‑71 (MQ=255)
                                      ttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagccag                              >  1:3296858/1‑71 (MQ=255)
                                       tGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagcc                                <  1:1474575/68‑1 (MQ=255)
                                       tGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagcc                                <  1:2288136/68‑1 (MQ=255)
                                       tGCCATTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTAt                                                   >  1:1832980/1‑49 (MQ=255)
                                          ccTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGc                          >  1:4594606/1‑71 (MQ=255)
                                          ccTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGc                          <  1:1856097/71‑1 (MQ=255)
                                            ttGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGt                        >  1:4556130/1‑71 (MQ=255)
                                             tGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGt                        >  1:3218542/1‑70 (MQ=255)
                                              gACTGGCAGCGTCCGGCTCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagcca                               >  1:2850527/1‑62 (MQ=255)
                                              gACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCa                                        >  1:436715/1‑53 (MQ=255)
                                              gACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagccaa                              >  1:3442579/1‑62 (MQ=255)
                                              gACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTCCAGCCAATGGCGCTa                      >  1:1517193/1‑71 (MQ=255)
                                                cTGGCAGCGTCCGGCGCGTCCCATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTAt                     >  1:706975/1‑70 (MQ=255)
                                                   gCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaaa                 <  1:4814578/71‑1 (MQ=255)
                                                   gCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATATCTATAACCa                                        >  1:2405054/1‑48 (MQ=255)
                                                    cAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAc                >  1:416713/1‑71 (MQ=255)
                                                    cAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAc                >  1:674545/1‑71 (MQ=255)
                                                     aGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAcc               <  1:3192181/71‑1 (MQ=255)
                                                         tCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaa                  <  1:1168531/64‑1 (MQ=255)
                                                         tCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaa                  <  1:3531251/64‑1 (MQ=255)
                                                           caccgcgCCAAATGAACAGCACCTCGTTTTTCTACAATCATTCCAGCCAATGGCGCTATGaaa                 <  1:4525448/60‑1 (MQ=255)
                                                           cGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTa                      >  1:4357240/1‑58 (MQ=255)
                                                            ggCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcgg        >  1:114987/1‑71 (MQ=255)
                                                               gcgcCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagccaa                              >  1:4170556/1‑45 (MQ=255)
                                                               gcgTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTAt                     <  1:2253670/55‑1 (MQ=255)
                                                               gcgTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcggcg      >  1:1445627/1‑70 (MQ=255)
                                                                cgTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGa    <  1:3839467/71‑1 (MQ=255)
                                                                  tCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAag  <  1:2050216/71‑1 (MQ=255)
                                                                  |                                                                      
AAAACTGCGTCCGCAAACCGGCTGGCAGCCGCTGGCGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAG  >  Exported/833288‑833424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: