Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 833,387 C→T 12.1% H615H (CAC→CAT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported833,3870CT12.1% 171.8 / 11.8 66H615H (CAC→CATnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (32/26);  new base T (4/4);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGC  >  Exported/833323‑833456
                                                                |                                                                     
cGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagc                                                                 <  1:1808947/71‑1 (MQ=255)
 gTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagcc                                                                <  1:5274879/71‑1 (MQ=255)
 ctttGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagcc                                                                <  1:3783453/70‑1 (MQ=255)
   ttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagccag                                                              >  1:926560/1‑71 (MQ=255)
   ttGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagccag                                                              >  1:3296858/1‑71 (MQ=255)
    tGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagcc                                                                <  1:2288136/68‑1 (MQ=255)
    tGCGCTCGACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagcc                                                                <  1:1474575/68‑1 (MQ=255)
       ccTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGc                                                          >  1:4594606/1‑71 (MQ=255)
       ccTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGc                                                          <  1:1856097/71‑1 (MQ=255)
         ttGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGt                                                        >  1:4556130/1‑71 (MQ=255)
          tGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGt                                                        >  1:3218542/1‑70 (MQ=255)
           gACTGGCAGCGTCCGGCTCGTCACATGAACAGCACTTCTTATTTCTATAACCACTccagcca                                                               >  1:2850527/1‑62 (MQ=255)
           gACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagccaa                                                              >  1:3442579/1‑62 (MQ=255)
           gACTGGAACCGACCACCGCGCCAAATGAACAGCACCTCGTTTTTCTACAATCATTCCAGCCAATGGCGCTa                                                      >  1:1517193/1‑71 (MQ=255)
             cTGGCAGCGTCCGGCGCGTCCCATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTAt                                                     >  1:706975/1‑70 (MQ=255)
                gCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaaa                                                 <  1:4814578/71‑1 (MQ=255)
                 cAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAc                                                >  1:416713/1‑71 (MQ=255)
                 cAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAc                                                >  1:674545/1‑71 (MQ=255)
                  aGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAcc                                               <  1:3192181/71‑1 (MQ=255)
                      tCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaa                                                  <  1:3531251/64‑1 (MQ=255)
                      tCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGaa                                                  <  1:1168531/64‑1 (MQ=255)
                        caccgcgCCAAATGAACAGCACCTCGTTTTTCTACAATCATTCCAGCCAATGGCGCTATGaaa                                                 <  1:4525448/60‑1 (MQ=255)
                        cGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTa                                                      >  1:4357240/1‑58 (MQ=255)
                         ggCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcgg                                        >  1:114987/1‑71 (MQ=255)
                            gcgcCAAATGAACAGCACCTCGTTTTTCTACAATCATTccagccaa                                                              >  1:4170556/1‑45 (MQ=255)
                            gcgTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTAt                                                     <  1:2253670/55‑1 (MQ=255)
                            gcgTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcggcg                                      >  1:1445627/1‑70 (MQ=255)
                             cgTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGa                                    <  1:3839467/71‑1 (MQ=255)
                               tCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAag                                  <  1:2050216/71‑1 (MQ=255)
                                cacaTGAACAGCACTTCTTATTTCTATAACCACTccag                                                                  <  1:5281818/38‑1 (MQ=255)
                                 acaTGAACAGCACTTCTTATTTCTATAACCACTccagcca                                                               >  1:5628058/1‑40 (MQ=255)
                                 acaTGAACAGCACTTCTTATTTCTATAACCACTccagcca                                                               >  1:5499084/1‑40 (MQ=255)
                                 acaTGAACAGCACTTCTTATTTCTATAACCACTccagcca                                                               >  1:1177539/1‑40 (MQ=255)
                                 acaTGAACAGCACTTCTTATTTCTATAACCACTccagcca                                                               >  1:1170920/1‑40 (MQ=255)
                                 acaTGAACAGCACTTCTTATTTCTATAACCACTccagcca                                                               >  1:547798/1‑40 (MQ=255)
                                 acaTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAAc                                                >  1:3051946/1‑55 (MQ=255)
                                 acaTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGaa                                   >  1:5126154/1‑68 (MQ=255)
                                 acaTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGaa                                   >  1:2999371/1‑68 (MQ=255)
                                  caTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcg                                         <  1:3812713/61‑1 (MQ=255)
                                  caTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGt                               <  1:3012132/71‑1 (MQ=255)
                                  aaTGAACAGCACCTCGTTTTTCTACAATCATTccagcca                                                               >  1:2136937/2‑39 (MQ=12)
                                   aTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGtt                              <  1:1978814/71‑1 (MQ=255)
                                   aTGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCCTTATGaa                                                  <  1:657668/51‑1 (MQ=255)
                                       aCAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTg                          <  1:1617511/71‑1 (MQ=255)
                                         aGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTc                        <  1:2841665/71‑1 (MQ=255)
                                          gCACTTCTTATTTCTATAACCACTACAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGtt                        >  1:4711858/1‑69 (MQ=255)
                                            aCTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCg                                              >  1:3057029/1‑46 (MQ=255)
                                             cttcttATTTCTATAACCACTCCAGCCAGTGGCGt                                                        <  1:3248070/35‑1 (MQ=255)
                                              ttcttATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGa                   >  1:1530185/1‑71 (MQ=255)
                                               tcttATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAag                                  <  1:113318/55‑1 (MQ=255)
                                               tcttATTTCTATAACCACTCCAGCCAGTGGCGATATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGAt                  >  1:2557337/1‑71 (MQ=255)
                                                    tttCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCgg             <  1:2378010/71‑1 (MQ=255)
                                                     ttCTATAACCACTCCAGCAAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTAGCTGTCACCg                    >  1:2553774/1‑63 (MQ=255)
                                                      tCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCAcggc                                       >  1:3386911/1‑43 (MQ=255)
                                                      tCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAaga                                 >  1:2577421/1‑49 (MQ=255)
                                                      tCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGc                            >  1:3410386/1‑54 (MQ=255)
                                                      tCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCAcc                     >  1:105985/1‑61 (MQ=255)
                                                      tCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGa            <  1:917333/70‑1 (MQ=255)
                                                       cTATAACCACTCCAGCCAGTGGCGTTATGAAACCGt                                             <  1:1583002/36‑1 (MQ=255)
                                                       cTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATgg                <  1:3805493/65‑1 (MQ=255)
                                                        tataACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATgg                <  1:2855517/64‑1 (MQ=255)
                                                        tataACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATgg                <  1:2848725/64‑1 (MQ=255)
                                                          taACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCTGACa          <  1:375061/68‑1 (MQ=255)
                                                             ccACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGaa                                   >  1:5581069/1‑40 (MQ=255)
                                                             ccACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATcc     >  1:1954275/1‑70 (MQ=255)
                                                               aCTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGc  >  1:1231010/1‑71 (MQ=255)
                                                                |                                                                     
CGTTTGCCCTTGACTGGCAGCGTCCGGCGCGTCACATGAACAGCACTTCTTATTTCTATAACCACTCCAGCCAGTGGCGTTATGAAACCGTCACGGCGGAAGAGTTGCTGTCACCGATGGCGGACAAATCCCGC  >  Exported/833323‑833456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: