| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 189,641 | A→G | 18.4% | I380V (ATT→GTT) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 189,641 | 0 | A | G | 18.4% | 146.3 / 23.8 | 65 | I380V (ATT→GTT) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (27/26); new base G (4/8); total (31/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
AAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCC > Exported/189572‑189706 | aaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATTCACAt > 1:2461359/1‑71 (MQ=255)aaaGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACAt > 1:1352376/1‑71 (MQ=255) aaGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGtt < 1:3372895/71‑1 (MQ=255) aGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTa < 1:3760592/71‑1 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:4224866/71‑1 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:1140448/71‑1 (MQ=255) aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:5328848/71‑1 (MQ=255) gTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTaa < 1:1430545/71‑1 (MQ=255) gTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:1564498/70‑1 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCg < 1:4468663/68‑1 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g > 1:2389013/1‑70 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g > 1:2828260/1‑70 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATAGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g < 1:3524215/70‑1 (MQ=255) gAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTa > 1:4136647/1‑71 (MQ=255) aaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATg < 1:3577523/71‑1 (MQ=255) aCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGa > 1:4396090/1‑71 (MQ=255) gCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGAc > 1:1814148/1‑70 (MQ=255) aTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACg > 1:3528236/1‑68 (MQ=255) gACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAgg > 1:969445/1‑71 (MQ=255) gACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGAACCACAtt > 1:3021550/1‑49 (MQ=255) cacaAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTa > 1:1910623/1‑46 (MQ=16) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g < 1:4881224/53‑1 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa > 1:4640438/1‑62 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt < 1:5283223/71‑1 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt < 1:1393737/71‑1 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCtt > 1:165013/1‑70 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACg < 1:5583990/59‑1 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTa < 1:5347006/71‑1 (MQ=255) caAAATGCTGGCGGCGGTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGAc < 1:3759399/58‑1 (MQ=255) aactGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAAc < 1:3267072/68‑1 (MQ=255) aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACg < 1:4168114/57‑1 (MQ=255) aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa > 1:4537899/1‑59 (MQ=255) actGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAAc > 1:2664385/3‑70 (MQ=255) aaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTaa < 1:4526349/42‑1 (MQ=255) aTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGa < 1:1993556/71‑1 (MQ=255) tGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTaaa > 1:384446/1‑41 (MQ=255) tGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGcc > 1:5052938/1‑71 (MQ=9) gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCta < 1:1625746/71‑2 (MQ=9) gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCta < 1:3187422/71‑2 (MQ=9) cggcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCttt < 1:3467769/71‑1 (MQ=255) gTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatga < 1:1687361/71‑1 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa > 1:374156/1‑43 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:5344576/1‑70 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:200255/1‑70 (MQ=255) tCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:676933/42‑1 (MQ=255) tCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:2421135/42‑1 (MQ=255) tCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:4873787/42‑1 (MQ=255) gCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAaga > 1:952053/1‑42 (MQ=255) gCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAaga > 1:3115107/1‑42 (MQ=255) gCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTat > 1:2311735/1‑63 (MQ=255) aGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAagag > 1:810037/1‑41 (MQ=255) ggCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACg > 1:4697611/1‑50 (MQ=255) ggCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGa > 1:3355102/1‑51 (MQ=255) ggCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATacca > 1:5404280/1‑71 (MQ=255) ttCGCTGATCCACATTAAAGGCGA‑GTATGACGAagag > 1:260674/1‑37 (MQ=255) ttCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATac > 1:1271987/1‑66 (MQ=255) gCTGATCCACAATAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccaccacc < 1:4619047/70‑1 (MQ=255) cTGATCCACATTAAAGGCGA‑TTATGACGAAGAGGCCTTTAACGAAGCCTTTatgat < 1:224533/56‑1 (MQ=255) atGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc < 1:4488805/70‑1 (MQ=9) atGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc < 1:4482478/70‑1 (MQ=9) atGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc > 1:3772016/2‑71 (MQ=9) tCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGccc < 1:3725837/71‑1 (MQ=255) tCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGccc < 1:5266652/71‑1 (MQ=255) tCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGcc < 1:1177215/70‑1 (MQ=255) ccACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatga > 1:4814425/1‑50 (MQ=255) | AAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCC > Exported/189572‑189706 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |