Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 834,062 C→T 14.6% G840G (GGC→GGT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported834,0620CT14.6% 180.0 / 18.9 75G840G (GGC→GGTnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (34/30);  new base T (4/7);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCG  >  Exported/834001‑834128
                                                             |                                                                  
cGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTggg                                                            >  1:4726129/1‑70 (MQ=255)
 gAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGt                                                           >  1:2200177/1‑70 (MQ=255)
   gcaGCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGCGTCATCTTGGCaaa                                                        <  1:540376/69‑1 (MQ=255)
   aaaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa                                                          >  1:1998257/1‑69 (MQ=255)
    aaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa                                                          >  1:3336904/1‑68 (MQ=255)
    aaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAg                                                       <  1:3145946/71‑1 (MQ=255)
    aaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAg                                                       <  1:2508654/71‑1 (MQ=255)
     aGCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGTCAAAGa                                                      <  1:729744/71‑1 (MQ=255)
      gCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCaaa                                                        <  1:1676309/68‑1 (MQ=255)
      gCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCaaa                                                        <  1:2248638/68‑1 (MQ=255)
        gACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGa                                                      >  1:3160282/1‑68 (MQ=255)
        gACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGa                                                      >  1:3940128/1‑68 (MQ=255)
         aCTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAAcc                                                  <  1:3537040/71‑1 (MQ=255)
          cTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGCTCATCTTGGCAAAGAAAc                                                   >  1:2743144/1‑69 (MQ=255)
            gggAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAACCGAc                                               >  1:4552370/1‑71 (MQ=255)
             ggAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAACCGAc                                               <  1:2783832/70‑1 (MQ=255)
             ggAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAACCGAc                                               <  1:2258563/70‑1 (MQ=255)
             ggAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc                                               <  1:2862128/70‑1 (MQ=255)
              gAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAACCGAcgt                                             <  1:2618031/71‑3 (MQ=255)
                 aTCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc                                               <  1:4122724/66‑1 (MQ=255)
                  tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt                                          <  1:2503888/70‑1 (MQ=255)
                   cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGa                                                      >  1:4100183/1‑57 (MQ=255)
                   cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt                                             >  1:1844271/1‑66 (MQ=255)
                   cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCa                                        >  1:5401601/1‑71 (MQ=255)
                    tACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGa                                                >  1:1545818/1‑62 (MQ=255)
                    tACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCAc                                       >  1:2226527/1‑71 (MQ=255)
                     aCAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCa                                        >  1:2529147/1‑69 (MQ=255)
                      cAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCAc                                       <  1:1865711/69‑1 (MQ=255)
                      cAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACg                                      >  1:1021126/1‑70 (MQ=255)
                      cAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGc                                     <  1:2239059/71‑1 (MQ=255)
                       aaaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCg                                                 <  1:4628610/58‑1 (MQ=255)
                       aaaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCg                                                 <  1:1063994/58‑1 (MQ=255)
                       aaaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCg                                                 <  1:252611/58‑1 (MQ=255)
                        aaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa                                                        >  1:645874/1‑50 (MQ=255)
                        aaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa                                                        >  1:1495186/1‑50 (MQ=255)
                              tCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATc                                            <  1:4375163/56‑1 (MQ=255)
                               cgcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc                             >  1:4370572/1‑70 (MQ=255)
                                gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt                                             >  1:1707007/1‑53 (MQ=255)
                                gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt                                             >  1:3755340/1‑53 (MQ=255)
                                gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc                             >  1:226926/1‑69 (MQ=255)
                                gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc                             >  1:2810237/1‑69 (MQ=255)
                                 cgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa                                                        >  1:5557138/1‑41 (MQ=255)
                                 cgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg                          >  1:1941701/1‑71 (MQ=255)
                                    agaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCa                                        >  1:4731918/1‑54 (MQ=255)
                                    agaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt                       <  1:2440358/71‑1 (MQ=255)
                                     gaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt                       <  1:1024992/70‑1 (MQ=255)
                                      aaaTTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa                                                        <  1:3509166/36‑1 (MQ=255)
                                      aaaTTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa                                                        <  1:4792115/36‑1 (MQ=255)
                                      aaaTTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa                                                        <  1:1826759/36‑1 (MQ=255)
                                      aaaTTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGa                     <  1:4965660/71‑1 (MQ=255)
                                        aTTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAAcc                                                  >  1:2216898/1‑40 (MQ=255)
                                          tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt                              <  1:1505163/58‑1 (MQ=255)
                                          tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt                              <  1:3799150/58‑1 (MQ=255)
                                          tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt                              <  1:2527302/58‑1 (MQ=255)
                                          tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActct                 >  1:2784750/1‑71 (MQ=255)
                                          tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActct                 >  1:101428/1‑71 (MQ=255)
                                          tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActc                  >  1:2851460/1‑70 (MQ=255)
                                                 aGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCt           >  1:5064887/1‑70 (MQ=255)
                                                 aGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCt           >  1:1922530/1‑70 (MQ=255)
                                                 aGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg          <  1:4679563/71‑1 (MQ=255)
                                                 aGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg          <  1:328831/71‑1 (MQ=255)
                                                 aGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg          <  1:1136116/71‑1 (MQ=255)
                                                  gtgtGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg          >  1:520391/1‑70 (MQ=255)
                                                  gtgtGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg          >  1:5307175/1‑70 (MQ=255)
                                                  gtgtGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg          >  1:1337189/1‑70 (MQ=255)
                                                    gtgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGc                                     >  1:183619/1‑41 (MQ=255)
                                                     tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg                          <  1:4672705/51‑1 (MQ=255)
                                                     tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACt      <  1:4136320/71‑1 (MQ=255)
                                                     tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACt      <  1:1331336/71‑1 (MQ=255)
                                                      gCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTg     >  1:5150514/1‑71 (MQ=255)
                                                       cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt                       <  1:1568460/52‑1 (MQ=255)
                                                       cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt                       <  1:1278332/52‑1 (MQ=255)
                                                       cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt                       <  1:2588099/52‑1 (MQ=255)
                                                       cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTgg    >  1:2060598/1‑71 (MQ=255)
                                                           ggCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGgcg  >  1:1227394/1‑69 (MQ=255)
                                                             |                                                                  
CGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCG  >  Exported/834001‑834128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: