Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 834,224 G→T 19.8% P894P (CCG→CCT narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported834,2240GT19.8% 159.1 / 20.2 65P894P (CCG→CCTnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (28/24);  new base T (4/9);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

AAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG  >  Exported/834155‑834294
                                                                     |                                                                      
aaaaGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCgg                                                                       >  1:220715/1‑71 (MQ=255)
    ggCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGa                                                                    >  1:1320910/1‑70 (MQ=255)
     gCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACt                                                                  <  1:2612768/71‑1 (MQ=255)
     gCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACt                                                                  <  1:2308008/71‑1 (MQ=255)
        aGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTAc                                                               <  1:1073281/71‑1 (MQ=255)
        aGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTAc                                                               <  1:4876532/71‑1 (MQ=255)
         gTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTa                                                                >  1:5092015/1‑69 (MQ=255)
         gTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTAc                                                               >  1:1805810/1‑70 (MQ=255)
              atcTGATTCCAGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAAc                                                           <  1:1171900/67‑1 (MQ=255)
              atcTGATTCCAGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAAc                                                           <  1:1531459/67‑1 (MQ=255)
              atcTGATTCCAGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAAc                                                           <  1:1759713/67‑1 (MQ=255)
                 tGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAAc                                                           <  1:3983531/66‑1 (MQ=255)
                   aTCCCAGGTAAAACCGCGCCACACATTATGGTCGTATAGCGCGATTATCCGGCGACTTACGAACGCtt                                                       <  1:1439717/68‑1 (MQ=255)
                   aTCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCtt                                                       <  1:1762731/68‑1 (MQ=255)
                   aTCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTAc                                                    >  1:1664951/1‑71 (MQ=255)
                   aTCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTAc                                                    >  1:3137129/1‑71 (MQ=255)
                       cAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACctct                                                <  1:2961830/71‑1 (MQ=255)
                        aGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTa                                               >  1:4131261/1‑71 (MQ=255)
                        aGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGc                                                         <  1:2274748/61‑1 (MQ=255)
                        aGGCAAAACCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACctcgc                                               <  1:1793489/71‑3 (MQ=255)
                         ggTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGaa                                                            >  1:4401786/1‑57 (MQ=255)
                          gTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCttt                                                      <  1:3205612/62‑1 (MQ=255)
                            aaaaCCTCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCgg                                           >  1:3373877/1‑71 (MQ=255)
                            aaaaCCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCgg                                           >  1:3264816/1‑71 (MQ=255)
                            aaaaCCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCgg                                           <  1:537943/71‑1 (MQ=255)
                            aaaaCCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCgg                                           >  1:2028724/1‑71 (MQ=255)
                            aaaaCCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCgg                                           >  1:4059875/1‑71 (MQ=255)
                                  gcgcCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTAc                                                    >  1:2475642/1‑56 (MQ=255)
                                  gcgcCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTAc                                                    >  1:2682170/1‑56 (MQ=255)
                                  gcgcCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCt                                     <  1:151129/71‑1 (MQ=255)
                                      cacacaTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTAc                                                    >  1:1043999/1‑52 (MQ=255)
                                        cacaTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACctc                                                 <  1:5323215/53‑1 (MQ=255)
                                         acaTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACctct                                                <  1:38355/53‑1 (MQ=255)
                                         acaTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATg                                 >  1:2989700/1‑68 (MQ=255)
                                          caTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTa                                               >  1:3775983/1‑53 (MQ=255)
                                           aTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGaa                                                            >  1:4049950/1‑39 (MQ=255)
                                           aTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGaa                                                            >  1:2688210/1‑39 (MQ=255)
                                           aTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGaa                                                            >  1:2117296/1‑39 (MQ=255)
                                           aTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGa                                                             >  1:1524131/1‑38 (MQ=255)
                                           aTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAAc                                                           <  1:4409047/40‑1 (MQ=255)
                                           aTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTAc                                                    >  1:4276509/1‑47 (MQ=255)
                                           aTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTAc                                                    >  1:4700031/1‑47 (MQ=255)
                                           aTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTAc                                                    >  1:1337985/1‑47 (MQ=255)
                                            ttATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGaaa                           <  1:276779/71‑1 (MQ=255)
                                               tGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTAt                                              >  1:1417611/1‑49 (MQ=255)
                                                 gTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCgg                      <  1:2863443/71‑1 (MQ=255)
                                                 gTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCgg                      <  1:512139/71‑1 (MQ=255)
                                                  tCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGGGAAAATCGGt                     <  1:918393/71‑1 (MQ=255)
                                                  tCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGt                     <  1:2213556/71‑1 (MQ=255)
                                                  tCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGt                     <  1:4618116/71‑1 (MQ=255)
                                                    gTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTaa                   >  1:2423121/1‑71 (MQ=255)
                                                      agagCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATg                 >  1:2292926/1‑71 (MQ=255)
                                                      agagCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATg                 >  1:1718139/1‑71 (MQ=255)
                                                       gagCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATgg                <  1:2331647/71‑1 (MQ=255)
                                                       gagCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATgg                <  1:2770130/71‑1 (MQ=255)
                                                        agCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGc               <  1:2732854/71‑1 (MQ=255)
                                                          cgcgATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAAt                         <  1:2180099/59‑1 (MQ=255)
                                                            cgATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGt            >  1:2191912/1‑70 (MQ=255)
                                                              aTTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCgg                      <  1:3378964/58‑1 (MQ=255)
                                                               ttATCCGGCGACTTACGAACGCTTTACCTCTATCGGc                                          >  1:3829941/1‑37 (MQ=255)
                                                               ttATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTaa                   >  1:5053776/1‑60 (MQ=255)
                                                               ttATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGt            >  1:2175296/1‑67 (MQ=255)
                                                               ttATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAg        >  1:4055910/1‑71 (MQ=255)
                                                                 aTCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCt                                     <  1:4876335/40‑1 (MQ=255)
                                                                 aTCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAgg       <  1:3738083/70‑1 (MQ=255)
                                                                    cGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAgg       <  1:4685043/67‑1 (MQ=255)
                                                                     ggCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTg  <  1:2770132/71‑1 (MQ=255)
                                                                     |                                                                      
AAAAGGCGAGTGCGACCTGATCCCAGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTG  >  Exported/834155‑834294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: