Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 189,656 G→A 15.7% D385N (GAC→AAC)  ldcC → lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported189,6560GA15.7% 127.6 / 12.4 51D385N (GAC→AAC) ldcClysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base G (23/20);  new base A (3/5);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

AACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTG  >  Exported/189586‑189719
                                                                       |                                                               
aaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATg                                                                 <  1:3577523/71‑1 (MQ=255)
 aCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGa                                                                >  1:4396090/1‑71 (MQ=255)
   gCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGAc                                                               >  1:1814148/1‑70 (MQ=255)
      aTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACg                                                              >  1:3528236/1‑68 (MQ=255)
         gACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAgg                                                        >  1:969445/1‑71 (MQ=255)
              acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                                                            >  1:4640438/1‑62 (MQ=255)
              acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCttt                                                   <  1:5283223/71‑1 (MQ=255)
              acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCttt                                                   <  1:1393737/71‑1 (MQ=255)
              acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCtt                                                    >  1:165013/1‑70 (MQ=255)
               caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACg                                                              <  1:5583990/59‑1 (MQ=255)
               caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTa                                                  <  1:5347006/71‑1 (MQ=255)
               caAAATGCTGGCGGCGGTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGAc                                                               <  1:3759399/58‑1 (MQ=255)
                 aactGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAAc                                                <  1:3267072/68‑1 (MQ=255)
                 aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACg                                                              <  1:4168114/57‑1 (MQ=255)
                 aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                                                            >  1:4537899/1‑59 (MQ=255)
                  actGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAAc                                                >  1:2664385/3‑70 (MQ=255)
                   aTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGa                                              <  1:1993556/71‑1 (MQ=255)
                       tGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAACGAAGcc                                          >  1:5052938/1‑71 (MQ=9)
                         gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAACGAAGCCta                                        <  1:3187422/71‑2 (MQ=9)
                         gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAACGAAGCCta                                        <  1:1625746/71‑2 (MQ=9)
                          cggcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCttt                                       <  1:3467769/71‑1 (MQ=255)
                              gTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatga                                   <  1:1687361/71‑1 (MQ=255)
                                 aTCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                                                            >  1:374156/1‑43 (MQ=255)
                                 aTCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg                                 >  1:5344576/1‑70 (MQ=255)
                                 aTCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg                                 >  1:200255/1‑70 (MQ=255)
                                  tCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                                                            <  1:676933/42‑1 (MQ=255)
                                  tCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                                                            <  1:4873787/42‑1 (MQ=255)
                                  tCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                                                            <  1:2421135/42‑1 (MQ=255)
                                    gCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAaga                                                          >  1:3115107/1‑42 (MQ=255)
                                    gCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAaga                                                          >  1:952053/1‑42 (MQ=255)
                                    gCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTat                                     >  1:2311735/1‑63 (MQ=255)
                                      aGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAagag                                                         >  1:810037/1‑41 (MQ=255)
                                       ggCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACg                                               >  1:4697611/1‑50 (MQ=255)
                                       ggCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGa                                              >  1:3355102/1‑51 (MQ=255)
                                       ggCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATacca                          >  1:5404280/1‑71 (MQ=255)
                                          ttCGCTGATCCACATTAAAGGCGAGTATGACGAagag                                                         >  1:260674/1‑37 (MQ=255)
                                          ttCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATac                            >  1:1271987/1‑66 (MQ=255)
                                             gCTGATCCACAATAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccaccacc                     <  1:4619047/70‑1 (MQ=255)
                                              cTGATCCACATTAAAGGCGATTATGACGAAGAGGCCTTTAACGAAGCCTTTatgat                                  <  1:224533/56‑1 (MQ=255)
                                              atGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc                   <  1:4488805/70‑1 (MQ=9)
                                              atGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc                   <  1:4482478/70‑1 (MQ=9)
                                              atGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc                   >  1:3772016/2‑71 (MQ=9)
                                                  tCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGccc               <  1:5266652/71‑1 (MQ=255)
                                                  tCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGccc               <  1:3725837/71‑1 (MQ=255)
                                                  tCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGcc                <  1:1177215/70‑1 (MQ=255)
                                                   ccACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatga                                   >  1:4814425/1‑50 (MQ=255)
                                                        ttAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGc                                           <  1:5376660/37‑1 (MQ=255)
                                                          aaaGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATc        >  1:5594564/1‑70 (MQ=255)
                                                              gCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAtt   >  1:3416724/1‑71 (MQ=255)
                                                               cGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAttg  <  1:3962792/71‑1 (MQ=255)
                                                               cGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAtt   <  1:3617773/70‑1 (MQ=255)
                                                                gAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccacca                       >  1:3986262/1‑49 (MQ=255)
                                                                       |                                                               
AACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTG  >  Exported/189586‑189719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: