| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 189,664 | G→A | 21.6% | E387E (GAG→GAA) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 189,664 | 0 | G | A | 21.6% | 79.1 / 14.6 | 37 | E387E (GAG→GAA) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (13/16); new base A (3/5); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGT > Exported/189595‑189729 | gACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAgg > 1:969445/1‑71 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt < 1:1393737/71‑1 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt < 1:5283223/71‑1 (MQ=255) acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCtt > 1:165013/1‑70 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTa < 1:5347006/71‑1 (MQ=255) aactGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAAc < 1:3267072/68‑1 (MQ=255) actGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAAc > 1:2664385/3‑70 (MQ=255) aTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGa < 1:1993556/71‑1 (MQ=255) tGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGcc > 1:5052938/1‑71 (MQ=9) gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCta < 1:1625746/71‑2 (MQ=9) gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCta < 1:3187422/71‑2 (MQ=9) cggcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCttt < 1:3467769/71‑1 (MQ=255) gTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatga < 1:1687361/71‑1 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:200255/1‑70 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg > 1:5344576/1‑70 (MQ=255) gCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTat > 1:2311735/1‑63 (MQ=255) aGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAagag > 1:810037/1‑41 (MQ=255) ggCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACg > 1:4697611/1‑50 (MQ=255) ggCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGa > 1:3355102/1‑51 (MQ=255) ggCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATacca > 1:5404280/1‑71 (MQ=255) ttCGCTGATCCACATTAAAGGCGA‑GTATGACGAagag > 1:260674/1‑37 (MQ=255) ttCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATac > 1:1271987/1‑66 (MQ=255) gCTGATCCACAATAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccaccacc < 1:4619047/70‑1 (MQ=255) cTGATCCACATTAAAGGCGA‑TTATGACGAAGAGGCCTTTAACGAAGCCTTTatgat < 1:224533/56‑1 (MQ=255) atGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc < 1:4482478/70‑1 (MQ=9) atGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc < 1:4488805/70‑1 (MQ=9) atGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc > 1:3772016/2‑71 (MQ=9) tCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGccc < 1:5266652/71‑1 (MQ=255) tCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGccc < 1:3725837/71‑1 (MQ=255) tCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGcc < 1:1177215/70‑1 (MQ=255) ccACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatga > 1:4814425/1‑50 (MQ=255) ttAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGc < 1:5376660/37‑1 (MQ=255) aaaGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATc > 1:5594564/1‑70 (MQ=255) gCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAtt > 1:3416724/1‑71 (MQ=255) cGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAttg < 1:3962792/71‑1 (MQ=255) cGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAtt < 1:3617773/70‑1 (MQ=255) gA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccacca > 1:3986262/1‑49 (MQ=255) aCGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAt > 1:2169557/1‑61 (MQ=255) aCGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTGTTGCTTc < 1:4718442/70‑1 (MQ=255) gAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGt < 1:2283506/71‑1 (MQ=255) | GACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTGTTGCTTCGGT > Exported/189595‑189729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |