Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 834,405 A→G 18.7% M955V (ATG→GTG)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported834,4050AG18.7% 134.3 / 22.9 64M955V (ATG→GTG) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (34/18);  new base G (7/5);  total (41/23)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAA  >  Exported/834336‑834473
                                                                     |                                                                    
aaCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAgaga                                                                      <  1:4914177/70‑1 (MQ=255)
 aCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatg                                                                    <  1:961108/71‑1 (MQ=255)
 aCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatg                                                                    <  1:2228065/71‑1 (MQ=255)
    acacCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATc                                                                 >  1:5476158/1‑71 (MQ=255)
     cacCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATcc                                                                <  1:5451545/71‑1 (MQ=255)
      acCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCt                                                               >  1:3485436/1‑71 (MQ=255)
        cAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATc                                                                 >  1:4108804/1‑67 (MQ=255)
        cAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTg                                                              >  1:983200/1‑70 (MQ=255)
        cAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGa                                                             >  1:2804152/1‑71 (MQ=255)
           aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTg                                                              <  1:3280953/67‑1 (MQ=255)
           aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTg                                                              <  1:3049245/67‑1 (MQ=255)
           aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac                                                          >  1:3238812/1‑71 (MQ=255)
           aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac                                                          >  1:3474654/1‑71 (MQ=255)
           aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac                                                          >  1:117271/1‑71 (MQ=255)
           aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac                                                          <  1:799572/71‑1 (MQ=255)
           aGCGGAAGGTCCGGCGAAAGGACAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac                                                          >  1:2200624/1‑71 (MQ=255)
             cGGAAGGTCCGGGGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGac                                                            >  1:4328888/1‑67 (MQ=255)
             cGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATTATCCTGACACTg                                                        <  1:5524038/71‑1 (MQ=255)
             cGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGac                                                            >  1:4090681/1‑67 (MQ=255)
               gAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGc                                                      <  1:3128083/71‑1 (MQ=255)
                aaGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGaca                                                           >  1:4045272/1‑65 (MQ=255)
                aaGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGac                                                            >  1:5615062/1‑64 (MQ=255)
                  ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc                                                   >  1:279286/1‑71 (MQ=255)
                  ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc                                                   >  1:2593234/1‑71 (MQ=255)
                  ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc                                                   >  1:2905575/1‑71 (MQ=255)
                  ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc                                                   >  1:1892218/1‑71 (MQ=255)
                  ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc                                                   >  1:5262326/1‑71 (MQ=255)
                      cGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa                                                >  1:2987085/1‑70 (MQ=255)
                            aaGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatgat                                                                  >  1:2462898/1‑46 (MQ=255)
                            aaGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatgat                                                                  >  1:2982068/1‑46 (MQ=255)
                            aaGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatgat                                                                  >  1:5111066/1‑46 (MQ=255)
                             aGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACg                                        >  1:1376679/1‑71 (MQ=255)
                                ccAGCCGATGCTGAACACCGCAATTGATGC‑‑CAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAg                                   <  1:482382/71‑1 (MQ=255)
                                  aGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAg                                   <  1:2406312/71‑1 (MQ=255)
                                    ccGATGATTGACACCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGaaa                                              >  1:246601/1‑58 (MQ=255)
                                        tGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTTAGGTAGcc                             >  1:3078595/1‑71 (MQ=255)
                                         gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa                                                <  1:3195754/51‑1 (MQ=255)
                                         gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa                                                <  1:4031152/51‑1 (MQ=255)
                                         gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa                                                <  1:1163626/51‑1 (MQ=255)
                                         gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa                                                <  1:2659106/51‑1 (MQ=255)
                                         gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa                                                <  1:2746630/51‑1 (MQ=255)
                                         gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACg                                        >  1:5672233/1‑59 (MQ=255)
                                         gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCg                            >  1:524524/1‑71 (MQ=255)
                                          cTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCAGAAACCAACGGTCAGGTAGCCGt                           >  1:2728700/1‑71 (MQ=255)
                                           ttgacacCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGt                           >  1:3927219/4‑70 (MQ=255)
                                             gacacCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGtca                         >  1:2513408/2‑68 (MQ=255)
                                             aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAg                               >  1:2180490/1‑64 (MQ=255)
                                             aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTg                          >  1:1513117/1‑69 (MQ=255)
                                             aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGa                         >  1:1088208/1‑70 (MQ=255)
                                             aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGa                         >  1:1921058/1‑70 (MQ=255)
                                              acacCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAc                                                    >  1:3256172/1‑42 (MQ=255)
                                                acCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGc                     <  1:5314949/71‑1 (MQ=255)
                                                acCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCACCGGTCAGGTAGCCGTGAAAGc                     <  1:5325265/71‑1 (MQ=255)
                                                acCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGc                     <  1:2065873/71‑1 (MQ=255)
                                                 ccGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGcg                    <  1:2907865/71‑2 (MQ=255)
                                                 ccGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGcg                    <  1:4829589/71‑2 (MQ=255)
                                                 ccGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGcc                    <  1:2940260/71‑1 (MQ=255)
                                                  cGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGcg                    <  1:2075062/70‑2 (MQ=255)
                                                    ccaTTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGCGTgg                 >  1:1319085/3‑71 (MQ=255)
                                                    ccaTTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGCGTgg                 >  1:4249939/3‑71 (MQ=255)
                                                     caTTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCaa                                          >  1:2852855/2‑45 (MQ=12)
                                                     caTTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCaa                                          >  1:3723102/2‑45 (MQ=12)
                                                     caTTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGCGTggc                <  1:2837042/70‑2 (MQ=255)
                                                       ttGATGCGGCAGAGATGATCCTGACACTGGCACCGGaaa                                              <  1:2889438/39‑1 (MQ=255)
                                                        tGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTg                  >  1:5306018/1‑66 (MQ=255)
                                                         gATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTg                  >  1:5443950/1‑65 (MQ=255)
                                                         gATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGc            >  1:3230775/1‑71 (MQ=255)
                                                         gATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGc            >  1:4143126/1‑71 (MQ=255)
                                                                 agagATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGc     >  1:1048035/1‑70 (MQ=255)
                                                                 agagATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCg    <  1:5142443/71‑1 (MQ=255)
                                                                     atgatCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGaa  <  1:5637538/69‑1 (MQ=255)
                                                                     |                                                                    
AACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAA  >  Exported/834336‑834473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: