| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 834,494 | G→C | 28.6% | T984T (ACG→ACC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 834,494 | 0 | G | C | 28.6% | 65.2 / 33.6 | 49 | T984T (ACG→ACC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (23/12); new base C (11/3); total (34/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
GGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAA > Exported/834425‑834561 | ggAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACg > 1:1766373/1‑70 (MQ=255) gAAACCAACGGTCATGTTGCAGTCAAAGCGTGGCAGGCGCTGGGCGAGATCACCGGGCGCGAACATACCCa > 1:1453460/1‑71 (MQ=255) gAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGc > 1:976663/1‑70 (MQ=255) gAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCa > 1:139084/1‑71 (MQ=255) gAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCa < 1:1737948/71‑1 (MQ=255) aCCAACGGTCATGTTGCAGTCAAAGCGTGGCAGGCGCTGGGCGAGATCACCGGGCGCGAACATACCCATCt > 1:836391/1‑71 (MQ=255) ccAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTg < 1:4765093/71‑1 (MQ=255) cAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTg < 1:4758660/70‑1 (MQ=255) aCGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGgcg < 1:1617288/71‑1 (MQ=255) cGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGgcg > 1:1671944/1‑70 (MQ=255) cGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGgcg > 1:1951297/1‑70 (MQ=255) gTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTg < 1:1907621/71‑1 (MQ=255) gTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGCCCATACGCATCTGGCGCTGAATa > 1:2461451/1‑70 (MQ=255) aGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTg > 1:331218/1‑64 (MQ=255) aGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATa > 1:5537256/1‑68 (MQ=255) gCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAaag > 1:3476574/1‑70 (MQ=255) aaaGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATaa > 1:3609774/1‑62 (MQ=255) aaaGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAagaaga > 1:5674681/1‑68 (MQ=255) aaaGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGAc > 1:1786923/1‑69 (MQ=255) aaaGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGAc > 1:2851789/1‑69 (MQ=255) aaGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACgag < 1:5475302/71‑1 (MQ=255) cTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCAGGCGCTGAATAAAGAAGACGAGAAGATCCGc > 1:4356105/1‑71 (MQ=255) cTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCcg > 1:2001859/1‑71 (MQ=255) gAGCGAATTTACCGGTCGTGACCATACGCATCTGGGGCTGAATAAAGAAGACGAGAAGATCCGCTTCcgc > 1:3195853/1‑70 (MQ=255) aGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGa < 1:1801173/71‑1 (MQ=255) aGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGa > 1:1934889/1‑71 (MQ=255) aGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGa < 1:2626879/71‑1 (MQ=255) aaTTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGatat > 1:3871637/1‑70 (MQ=255) aaTTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATAtt < 1:3332867/71‑1 (MQ=255) aTTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATAtt > 1:3796856/1‑70 (MQ=255) taacGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCa > 1:4472993/4‑69 (MQ=255) tACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACgaga > 1:2373374/1‑47 (MQ=255) tACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCa > 1:1068834/1‑69 (MQ=255) cGGGCGCGAACATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGata < 1:266052/62‑1 (MQ=255) cGGGCGCGAACATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGcg > 1:5275372/1‑69 (MQ=255) cGGGCGCGAACATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGcg > 1:571306/1‑69 (MQ=255) ggTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGcaca < 1:1678312/71‑1 (MQ=255) cGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGata > 1:2690224/1‑58 (MQ=255) aacATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcg > 1:5178234/3‑70 (MQ=255) acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGc > 1:2943682/2‑65 (MQ=255) acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGc > 1:650790/2‑65 (MQ=255) acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcgt > 1:1830055/2‑69 (MQ=255) acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcgt > 1:4818750/2‑69 (MQ=255) acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcgt > 1:363581/2‑69 (MQ=255) cATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCaa < 1:5646884/71‑1 (MQ=255) cATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTaa < 1:4841285/71‑1 (MQ=255) cATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTaa < 1:4857332/71‑1 (MQ=255) aTACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCg < 1:2931192/40‑1 (MQ=255) tACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTaaaa > 1:4450702/1‑71 (MQ=255) cGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCcgc > 1:1926493/1‑63 (MQ=255) | GGAAACCAACGGTCAGGTAGCCGTGAAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAA > Exported/834425‑834561 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |