Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 834,515 A→G 47.8% E991E (GAA→GAG narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported834,5150AG47.8% 2.1 / 58.0 46E991E (GAA→GAGnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (15/9);  new base G (14/8);  total (29/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCT  >  Exported/834450‑834584
                                                                 |                                                                     
aaaGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAagaaga                                                                     >  1:5674681/1‑68 (MQ=255)
aaaGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGAc                                                                    >  1:2851789/1‑69 (MQ=255)
aaaGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGAc                                                                    >  1:1786923/1‑69 (MQ=255)
 aaGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACgag                                                                 <  1:5475302/71‑1 (MQ=255)
          cTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCAGGCGCTGAATAAAGAAGACGAGAAGATCCGc                                                        >  1:4356105/1‑71 (MQ=255)
               cTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCcg                                                   >  1:2001859/1‑71 (MQ=255)
                 gAGCGAATTTACCGGTCGTGACCATACGCATCTGGGGCTGAATAAAGAAGACGAGAAGATCCGCTTCcgc                                                  >  1:3195853/1‑70 (MQ=255)
                  aGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGa                                                <  1:2626879/71‑1 (MQ=255)
                  aGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGa                                                <  1:1801173/71‑1 (MQ=255)
                  aGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGa                                                >  1:1934889/1‑71 (MQ=255)
                      aaTTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGatat                                             >  1:3871637/1‑70 (MQ=255)
                      aaTTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATAtt                                            <  1:3332867/71‑1 (MQ=255)
                       aTTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATAtt                                            >  1:3796856/1‑70 (MQ=255)
                          taacGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCa                                          >  1:4472993/4‑69 (MQ=255)
                          tACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACgaga                                                                >  1:2373374/1‑47 (MQ=255)
                          tACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCa                                          >  1:1068834/1‑69 (MQ=255)
                             cGGGCGCGAACATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGata                                              <  1:266052/62‑1 (MQ=255)
                             cGGGCGCGAACATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGcg                                      >  1:5275372/1‑69 (MQ=255)
                             cGGGCGCGAACATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGcg                                      >  1:571306/1‑69 (MQ=255)
                              ggTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGcaca                                    <  1:1678312/71‑1 (MQ=255)
                                 cGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGata                                              >  1:2690224/1‑58 (MQ=255)
                                     aacATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcg                              >  1:5178234/3‑70 (MQ=255)
                                      acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGc                                  >  1:650790/2‑65 (MQ=255)
                                      acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGc                                  >  1:2943682/2‑65 (MQ=255)
                                      acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcgt                             >  1:4818750/2‑69 (MQ=255)
                                      acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcgt                             >  1:1830055/2‑69 (MQ=255)
                                      acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcgt                             >  1:363581/2‑69 (MQ=255)
                                       cATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCaa                           <  1:5646884/71‑1 (MQ=255)
                                       cATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTaa                           <  1:4841285/71‑1 (MQ=255)
                                       cATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTaa                           <  1:4857332/71‑1 (MQ=255)
                                        aTACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCg                                                         <  1:2931192/40‑1 (MQ=255)
                                         tACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTaaaa                         >  1:4450702/1‑71 (MQ=255)
                                           cccATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAAtt                       <  1:1796395/69‑1 (MQ=255)
                                           cGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCcgc                               >  1:1926493/1‑63 (MQ=255)
                                            ccATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTa                      <  1:1309694/70‑1 (MQ=255)
                                               tCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAg                                         <  1:4613337/49‑1 (MQ=255)
                                                 tGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGatat                                             >  1:2538345/1‑43 (MQ=19)
                                                 tGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGat                                               >  1:4701327/1‑41 (MQ=19)
                                                 tGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGat                                               >  1:576717/1‑41 (MQ=19)
                                                    cgcTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAAtt                       <  1:4708290/62‑1 (MQ=255)
                                                        gAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGa          >  1:2512401/1‑71 (MQ=255)
                                                         cacaaaGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCAc         >  1:2355412/4‑71 (MQ=255)
                                                         aaTAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCg           <  1:1233782/69‑1 (MQ=255)
                                                         aaTAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGa          <  1:4425568/70‑1 (MQ=255)
                                                          acaaaGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCAcc        <  1:5475291/69‑1 (MQ=255)
                                                           tAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCcgc                               >  1:632745/1‑47 (MQ=255)
                                                            aaaGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTAtc                    >  1:3525364/1‑57 (MQ=255)
                                                             aagaagACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCaaaa                         >  1:2592900/1‑51 (MQ=255)
                                                             aagaagACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCaaaa                         >  1:3180791/1‑51 (MQ=255)
                                                              agaggaCGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACctggt    >  1:1276809/1‑71 (MQ=255)
                                                               gaagaCGAGAAGATCCGCTTCCGCGATATTCAGGc                                       <  1:4392049/35‑1 (MQ=255)
                                                               gaagaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCaaaa                         <  1:2206360/49‑1 (MQ=255)
                                                               gaagaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctg      >  1:1353679/1‑68 (MQ=255)
                                                               gaagaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACct       >  1:5152866/1‑67 (MQ=255)
                                                                aagaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtct  >  1:3823411/1‑71 (MQ=255)
                                                                 |                                                                     
AAAGCCTGGGCTGCCCTGAGCGAATTTACCGGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCT  >  Exported/834450‑834584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: