Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 834,608 T→C 17.2% S1022S (TCT→TCC narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported834,6080TC17.2% 188.7 / 30.4 87S1022S (TCT→TCCnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (43/29);  new base C (8/7);  total (51/36)
Fisher's exact test for biased strand distribution p-value = 7.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

ATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACT  >  Exported/834538‑834676
                                                                      |                                                                    
atatTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCt                                                                      <  1:4720274/71‑1 (MQ=255)
   ttCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTa                                                                    >  1:3980592/1‑70 (MQ=255)
     cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACaa                                                                 >  1:913106/1‑71 (MQ=255)
     cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACa                                                                  <  1:3276936/70‑1 (MQ=255)
     cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACa                                                                  <  1:19916/70‑1 (MQ=255)
         cacaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGcc                                                             >  1:5485810/1‑71 (MQ=255)
          gcaGCCGCGTAAAATTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGcgg                                                            <  1:1426495/70‑3 (MQ=255)
          acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg                                                            >  1:775879/1‑71 (MQ=255)
          acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg                                                            >  1:2810182/1‑71 (MQ=255)
          acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg                                                            >  1:3431265/1‑71 (MQ=255)
            aGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGt                                                          <  1:3199465/71‑1 (MQ=255)
            aGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGt                                                          <  1:4438733/71‑1 (MQ=255)
                  gcAAAATTATCTCCAGCCCGCCCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTacac                                                     >  1:1316838/1‑70 (MQ=255)
                  gcAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTacac                                                     >  1:4136311/1‑70 (MQ=255)
                   taaaaTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACAcc                                                    >  1:2529333/2‑70 (MQ=255)
                   cAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACa                                                                  >  1:1576026/1‑56 (MQ=255)
                    aaaaTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCggg                                                           >  1:2979045/1‑62 (MQ=255)
                     aaaTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAAc                                                 >  1:692219/1‑71 (MQ=255)
                      aaTTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACg                                                >  1:5147618/1‑71 (MQ=255)
                        ttATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGtt                                              <  1:4566101/71‑1 (MQ=255)
                           tctcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCAc                                           >  1:422689/1‑71 (MQ=255)
                           tctcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCAc                                           >  1:3109015/1‑71 (MQ=255)
                               cAGCCCGACCTGGTCTGGTCTGGAAGATGAACATGTTTCTTACAACGCCGGTTACACCAACGTTCAc                                           <  1:3874263/67‑1 (MQ=255)
                               cAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAga                                       <  1:3325402/71‑2 (MQ=255)
                               cAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGc                                       >  1:2279472/1‑71 (MQ=255)
                               cAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGc                                       >  1:3771642/1‑71 (MQ=255)
                               cAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAgt                                       >  1:3245122/1‑70 (MQ=255)
                                aGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAgtt                                      <  1:3154329/71‑3 (MQ=255)
                                    cGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGa                                         >  1:5564691/1‑64 (MQ=255)
                                      aCCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc                                 >  1:3053275/1‑70 (MQ=255)
                                       ccTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                               <  1:2859978/71‑1 (MQ=255)
                                        ctggtccggtCTGGAAAGCTATCACGTCTCCTATAACGCGGGATACACCAACGtt                                              <  1:1773061/55‑1 (MQ=255)
                                        ctggtccggtCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGtt                                              <  1:4670662/55‑1 (MQ=255)
                                         tggtctggtcTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATg                             >  1:4345503/1‑71 (MQ=255)
                                           gtctggtctgGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTg                                     <  1:4221275/61‑1 (MQ=255)
                                           gtctggtctgGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGc                           >  1:1666562/1‑71 (MQ=255)
                                           gtccggtctgGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTgg                            <  1:4443603/70‑1 (MQ=255)
                                             ctggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg                          >  1:1296049/1‑70 (MQ=255)
                                             ccggtctggAAAGAGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGc                           >  1:2991480/3‑69 (MQ=255)
                                              tggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                               <  1:997608/64‑1 (MQ=255)
                                              tggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                               <  1:1082990/64‑1 (MQ=255)
                                              tggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                               <  1:1249383/64‑1 (MQ=255)
                                              tggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                               <  1:1256267/64‑1 (MQ=255)
                                               ggtctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCAc                       <  1:4005560/71‑1 (MQ=255)
                                                gtctggAAGATGAACACGTTTCTTACAACGCCGGTTaca                                                      >  1:4015644/1‑39 (MQ=255)
                                                gtctggAAGATGAACACGTTTCTTACAACGCCGGTTaca                                                      >  1:3717928/1‑39 (MQ=255)
                                                gtctggAAGATGAACACGTTTCTTACAACGCCGGTTa                                                        >  1:1806213/1‑37 (MQ=255)
                                                gtctggAAGATGAACACGTTTCTTACAACGCCGGTTa                                                        >  1:4715456/1‑37 (MQ=255)
                                                gtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGt                         <  1:191628/68‑1 (MQ=255)
                                                gtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTAc                       <  1:4367756/70‑1 (MQ=255)
                                                gtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTAc                       <  1:3967120/70‑1 (MQ=255)
                                                gtctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACg                      >  1:4241321/1‑71 (MQ=255)
                                                 tctggATGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTg                                     <  1:3257601/55‑1 (MQ=255)
                                                  ctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGct                    >  1:2064287/1‑71 (MQ=255)
                                                   tggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGc                     <  1:4808438/69‑1 (MQ=255)
                                                     gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc                 >  1:5173350/1‑71 (MQ=255)
                                                     gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc                 <  1:1157887/71‑1 (MQ=255)
                                                     gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc                 <  1:1764458/71‑1 (MQ=255)
                                                       aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg               <  1:1013436/71‑1 (MQ=255)
                                                        gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc                                 <  1:5523829/52‑1 (MQ=255)
                                                          tGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctcac                 >  1:223304/1‑64 (MQ=255)
                                                           gAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc                                 <  1:4209999/49‑1 (MQ=255)
                                                           gAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcg           >  1:1469327/1‑71 (MQ=255)
                                                            aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt          <  1:2843237/71‑1 (MQ=255)
                                                             acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                               <  1:2962516/49‑1 (MQ=255)
                                                             acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                               <  1:2550197/49‑1 (MQ=255)
                                                             acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt          >  1:4333227/1‑70 (MQ=255)
                                                               acGTTTCTTACAACGCCGGTTACACCAACGTTCACGAg                                        >  1:4555694/1‑38 (MQ=255)
                                                               acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAg       >  1:5009384/1‑71 (MQ=255)
                                                               acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAg       <  1:266260/71‑1 (MQ=255)
                                                               acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAg       >  1:1235246/1‑71 (MQ=255)
                                                                cGTTTCTTACAACGCCGGTTACACCAACGTTCACGAg                                        >  1:178022/1‑37 (MQ=255)
                                                                cGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAg       >  1:1435995/1‑70 (MQ=255)
                                                                cGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc      <  1:2199704/71‑1 (MQ=255)
                                                                  tttCTTACAACGCCGGTTACACCAACTTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc      <  1:135404/69‑1 (MQ=255)
                                                                  tttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATgg                            <  1:3958168/47‑1 (MQ=255)
                                                                  tttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa    >  1:538620/1‑71 (MQ=255)
                                                                   ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATc                                  >  1:1707135/1‑40 (MQ=255)
                                                                   ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc      >  1:814090/1‑68 (MQ=255)
                                                                   ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc      >  1:4392780/1‑68 (MQ=255)
                                                                   ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc      >  1:2614700/1‑68 (MQ=255)
                                                                   ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc      >  1:2621404/1‑68 (MQ=255)
                                                                    tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                               >  1:3060372/1‑42 (MQ=255)
                                                                    tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                               >  1:174100/1‑42 (MQ=255)
                                                                    tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGc                     >  1:5035308/1‑52 (MQ=255)
                                                                    tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt  >  1:1481969/1‑71 (MQ=255)
                                                                     cTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgt             >  1:2537173/1‑59 (MQ=255)
                                                                     cTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgt             >  1:3420004/1‑59 (MQ=255)
                                                                      ttACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg               >  1:1369880/1‑56 (MQ=255)
                                                                      ttACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg               >  1:863518/1‑56 (MQ=255)
                                                                      ttACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg               >  1:1440838/1‑56 (MQ=255)
                                                                      ttACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg               >  1:2963754/1‑56 (MQ=255)
                                                                      |                                                                    
ATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACT  >  Exported/834538‑834676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: