| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 28,911 | T→C | 6.5% | I139I (ATT→ATC) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 28,911 | 0 | T | C | 6.5% | 272.5 / 7.4 | 92 | I139I (ATT→ATC) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base T (27/59); new base C (2/4); total (29/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTGACGTGG > Exported/28852‑28981 | gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTgg > 1:4129867/1‑62 (MQ=255)gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTgg > 1:3165114/1‑62 (MQ=255)gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTgg > 1:65555/1‑62 (MQ=255)gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAAc > 1:5090228/1‑71 (MQ=255)gCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAAc > 1:5096366/1‑71 (MQ=255) cGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACcg < 1:3218512/71‑1 (MQ=255) cGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACcg < 1:2562713/71‑1 (MQ=255) cGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACcg < 1:276823/71‑1 (MQ=255) gATTGATAAAGCCGAAGACCGCCGCCGTTTCGACGTAGCGATGAAGAAAATCGGTCTGGAAACTGCGCGtt < 1:4608453/71‑1 (MQ=255) gATTGATAAAGCCGAAGACCGCCGCCGTTTCGACGTAGCGATGAAGAAAATCGGTCTGGAAACTGCGCGtt < 1:2994552/71‑1 (MQ=255) gATTGATAAAGCCGAAGACCGCCGCCGTTTCGACGTAGCGATGAAGAAAATCGGTCTGGAAACTGCGCGtt < 1:1636454/71‑1 (MQ=255) gATTGATAAAGCCGAAGACCGCCGCCGTTTCGACGTAGCGATGAAGAAAATCGGTCTGGAAACTGCGCGtt < 1:1182086/71‑1 (MQ=255) gATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGa < 1:4730732/60‑1 (MQ=255) gATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGa < 1:4204599/60‑1 (MQ=255) gATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGa < 1:524396/60‑1 (MQ=255) gATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGa < 1:1436414/60‑1 (MQ=255) tGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTcc < 1:1750081/70‑1 (MQ=255) tGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCg > 1:146173/1‑71 (MQ=255) tGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCg > 1:2555469/1‑71 (MQ=255) tGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCg > 1:2551023/1‑71 (MQ=255) tGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCg > 1:2089491/1‑71 (MQ=255) tGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCg > 1:677694/1‑71 (MQ=255) tGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCg > 1:1008019/1‑71 (MQ=255) gaCCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGcacaca > 1:1217099/1‑70 (MQ=255) gaCCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac < 1:3464702/71‑1 (MQ=255) gaCCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac < 1:2272895/71‑1 (MQ=255) gaCCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac > 1:2676646/1‑71 (MQ=255) gaCCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac < 1:3622444/71‑1 (MQ=255) gaCCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac > 1:1140989/1‑71 (MQ=255) gccgTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATg < 1:4803010/71‑1 (MQ=255) cgtcgtTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGga < 1:1928291/71‑1 (MQ=255) tttCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTcc < 1:3267615/46‑1 (MQ=255) tttCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTcc < 1:5124889/46‑1 (MQ=255) tttCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTcc < 1:3289423/46‑1 (MQ=255) tttCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTcc < 1:1964796/46‑1 (MQ=255) tttCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTcc < 1:3993887/46‑1 (MQ=255) tttCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTcc < 1:2621692/46‑1 (MQ=255) tttCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTcc < 1:2996953/46‑1 (MQ=255) tttCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTcc < 1:134618/46‑1 (MQ=255) tttCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGaagaag < 1:3676140/71‑1 (MQ=255) tCGACGTAGCGATGAAGAAAATTGGTCTGGAAACcgcgcg > 1:2294053/1‑40 (MQ=255) tCGACGTAGCGATGAAGAAAATTGGTCTGGAAACcgcgcg > 1:878201/1‑40 (MQ=255) tCGACGTAGCGATGAAGAAAATTGGTCTGGAAACcgcgcg > 1:2794611/1‑40 (MQ=255) tCGACGTAGCGATGAAGAAAATTGGTCTGGAAACcgcgcg > 1:270674/1‑40 (MQ=255) tCGACGTAGCGATGAAGAAAATTGGTCTGGAAACcgcgcg > 1:4189232/1‑40 (MQ=255) tCGACGTAGCGATGAAGAAAATTGGTCTGGAAACcgcgc > 1:1674075/1‑39 (MQ=255) tCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCgg > 1:3483509/1‑46 (MQ=255) tCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCgg > 1:1996645/1‑46 (MQ=255) gACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTcc < 1:89374/42‑1 (MQ=255) gATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAgcgc > 1:1317467/1‑62 (MQ=255) aTGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACg < 1:992900/48‑1 (MQ=255) aTGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACg < 1:3133309/48‑1 (MQ=255) aTGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACg < 1:316144/48‑1 (MQ=255) aTGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACg < 1:1025970/48‑1 (MQ=255) aTGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACg < 1:1136480/48‑1 (MQ=255) aTGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACg < 1:1636423/48‑1 (MQ=255) aTGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACg < 1:2442502/48‑1 (MQ=255) aTGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACg < 1:2537555/48‑1 (MQ=255) tGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCg < 1:4483405/39‑1 (MQ=255) tGAAGAAAATTGGTCTGGAAACCGCGCGGTCCGGTATCg < 1:4060593/39‑1 (MQ=255) gaagaaAATTGGTCTGGAAACCGCGCGTTCCGGTATCg < 1:3078472/38‑1 (MQ=255) gaagaaAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGAt > 1:2974155/1‑48 (MQ=255) agaaAATTGGTCTGGAGACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAgc < 1:4129231/55‑1 (MQ=255) agaaAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAgc < 1:4121973/55‑1 (MQ=255) agaaAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAgc < 1:2239594/55‑1 (MQ=255) agaaAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAgc < 1:2226142/55‑1 (MQ=255) agaaAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAgc < 1:631210/55‑1 (MQ=255) agaaAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAgc < 1:739575/55‑1 (MQ=255) agaaAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAgc < 1:4115996/55‑1 (MQ=255) agaaAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAgc < 1:852311/55‑1 (MQ=255) agaaAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCt > 1:5057065/1‑71 (MQ=255) gaaAATTGGTCTGGAAACCGCGCGTTCCGGTATTGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCt > 1:2194469/1‑70 (MQ=255) gaaAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAgc < 1:267098/54‑1 (MQ=255) aaaaTTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTGa < 1:1134232/71‑1 (MQ=255) aaaaTTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTGa < 1:3637903/71‑1 (MQ=255) aaaTTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTGa < 1:2608899/70‑1 (MQ=255) aaaTTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTGa < 1:1903524/70‑1 (MQ=255) aaaTTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTGa < 1:3180745/70‑1 (MQ=255) aaaTTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGCTGCCGCTGa < 1:357904/70‑1 (MQ=255) aaaTCGGTCTGGAAACTGCGCGTTCCGGTATCGCTCATTCAATGGAAGAAGCGCTGGCGGTTGCCGCTg > 1:726927/1‑69 (MQ=255) aaaTCGGTCTGGAAACTGCGCGTTCCGGTATCGCTCATTCAATGGAAGAAGCGCTGGCGGTTGCCGCTg > 1:4187457/1‑69 (MQ=255) aaTTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGaagaag > 1:2279819/1‑50 (MQ=255) aTTGGTCTGGAAACCGCGCGTTCCGGTATCgcacgcac < 1:2012957/38‑1 (MQ=255) aTTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac < 1:3411583/38‑1 (MQ=255) aTTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac < 1:1027191/38‑1 (MQ=255) aTTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac < 1:4045895/38‑1 (MQ=255) aTTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac < 1:4052016/38‑1 (MQ=255) aTTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac < 1:3040248/38‑1 (MQ=255) aTTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac < 1:2249016/38‑1 (MQ=255) aTTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac < 1:3077716/38‑1 (MQ=255) aTTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac < 1:5060162/38‑1 (MQ=255) aTTGGTCTGGAAACCGCGCGTTCCGGTATCGCacacac < 1:4524333/38‑1 (MQ=255) ttGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGcag > 1:2537532/1‑64 (MQ=255) ttGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTg > 1:734893/1‑66 (MQ=255) ttGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTg > 1:350961/1‑66 (MQ=255) tGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTGACGTgg > 1:4295924/1‑71 (MQ=255) tGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTGACGTgg > 1:4414702/1‑71 (MQ=255) | GCCGATGCGATTGATAAAGCAGAAGACCGCCGTCGTTTCGACGTAGCGATGAAGAAAATTGGTCTGGAAACCGCGCGTTCCGGTATCGCACACACGATGGAAGAAGCGCTGGCGGTTGCCGCTGACGTGG > Exported/28852‑28981 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |