Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,614,480 C→T 24.2% intergenic (‑105/+112) eutH ← / ← eutG predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,614,4800CT24.2% 67.1 / 16.0 33intergenic (‑105/+112)eutH/eutGpredicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base C (12/13);  new base T (3/5);  total (15/18)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCA  >  Exported/1614414‑1614547
                                                                  |                                                                   
gcggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCAt                                                                 >  1:413232/1‑71 (MQ=39)
 cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc                                                                >  1:1548697/1‑71 (MQ=39)
 cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc                                                                >  1:503915/1‑71 (MQ=39)
 cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc                                                                >  1:5023755/1‑71 (MQ=39)
 cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc                                                                >  1:4821866/1‑71 (MQ=39)
     gTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc                                                            <  1:1030584/71‑1 (MQ=39)
     gTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc                                                            <  1:495362/71‑1 (MQ=39)
        aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt                                                         >  1:3753546/1‑71 (MQ=38)
        aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt                                                         >  1:270339/1‑71 (MQ=38)
           gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc                                                      <  1:400448/71‑1 (MQ=255)
           gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc                                                      <  1:3494483/71‑1 (MQ=255)
           gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc                                                      <  1:4344863/71‑1 (MQ=255)
           gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc                                                      <  1:638910/71‑1 (MQ=255)
             cTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGc                                                      <  1:284218/69‑1 (MQ=11)
             cTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGc                                                      <  1:645112/69‑1 (MQ=11)
             cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc                                                    <  1:3337926/71‑1 (MQ=255)
             cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc                                                    >  1:677866/1‑71 (MQ=255)
             cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc                                                    <  1:3801480/71‑1 (MQ=255)
             cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc                                                    <  1:1768806/71‑1 (MQ=255)
                aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc                                                 >  1:1454422/1‑71 (MQ=255)
                aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcg                                                  >  1:4521302/1‑70 (MQ=255)
                   cGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCgcgc                                               <  1:1875160/70‑1 (MQ=255)
                         aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc                                        >  1:3971104/1‑71 (MQ=255)
                          cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg                                       >  1:3481713/1‑71 (MQ=14)
                          cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg                                       >  1:2517284/1‑71 (MQ=14)
                          cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg                                       >  1:711761/1‑71 (MQ=14)
                          cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc                                        <  1:2539557/70‑1 (MQ=255)
                                         aGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAAc                         <  1:322710/70‑1 (MQ=255)
                                              ggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa                   <  1:4770001/71‑1 (MQ=18)
                                              ggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa                   <  1:2912512/71‑1 (MQ=18)
                                              ggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa                   <  1:863468/71‑1 (MQ=18)
                                                       gcgGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTa          >  1:1899153/1‑71 (MQ=255)
                                                               cGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCa  <  1:2609667/71‑1 (MQ=255)
                                                                  |                                                                   
GCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTATCCGCCCTACATGTGCA  >  Exported/1614414‑1614547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: