| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,755,953 | G→A | 10.6% | D181N (GAC→AAC) | pssA → | phosphatidylserine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,755,953 | 0 | G | A | 10.6% | 295.9 / 19.4 | 104 | D181N (GAC→AAC) | pssA | phosphatidylserine synthase |
| Reads supporting (aligned to +/- strand): ref base G (41/52); new base A (5/6); total (46/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 104 total aligned reads displayed.
TACCTGCATCAGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATCGTCTGGATGATGTTA > Exported/1755887‑1756022 | tACCTGCATCAGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACAt > 1:1692121/1‑71 (MQ=255) aTCAGCACGATAAATATCTCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGttt < 1:3990757/71‑1 (MQ=255) aTCAGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGttt < 1:876331/71‑1 (MQ=255) aTCAGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGttt < 1:5170496/71‑1 (MQ=255) aTCAGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGttt < 1:5145295/71‑1 (MQ=255) tCAGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTg < 1:1129656/71‑1 (MQ=255) tCAGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTg < 1:4773496/71‑1 (MQ=255) aGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGaa > 1:1870273/1‑71 (MQ=255) aGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGaa > 1:1647525/1‑71 (MQ=255) cGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGttt > 1:2047079/1‑64 (MQ=255) cGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGttt > 1:3446132/1‑64 (MQ=255) cGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGttt > 1:4776238/1‑64 (MQ=255) cGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGttt > 1:4968069/1‑64 (MQ=255) cGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGttt > 1:143086/1‑64 (MQ=255) cGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGttt > 1:856399/1‑64 (MQ=255) cGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGttt > 1:836342/1‑64 (MQ=255) cGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTg < 1:5067912/65‑1 (MQ=255) cGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTg < 1:3726004/65‑1 (MQ=255) cGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTg < 1:3991785/65‑1 (MQ=255) gATAAATATCTCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGt > 1:3794226/1‑71 (MQ=255) gATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGt > 1:2011955/1‑71 (MQ=255) gATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGt > 1:4511501/1‑71 (MQ=255) gATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGt > 1:2624236/1‑71 (MQ=255) aTAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGt > 1:3254276/1‑70 (MQ=255) aTAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGt > 1:4696851/1‑70 (MQ=255) aTAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGt > 1:3813178/1‑70 (MQ=255) aaaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGa > 1:2462701/1‑62 (MQ=255) aaaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGATTGGGTTa > 1:588492/1‑70 (MQ=255) aaaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTa > 1:3059342/1‑70 (MQ=255) aaaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTa > 1:461052/1‑70 (MQ=255) aaaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTa > 1:1881917/1‑70 (MQ=255) aaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTa < 1:4055876/69‑1 (MQ=255) aaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTa < 1:3933663/69‑1 (MQ=255) aaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTa < 1:4217053/69‑1 (MQ=255) aaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTa < 1:4697564/69‑1 (MQ=255) aaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTa < 1:4770206/69‑1 (MQ=255) aaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTa < 1:1232087/69‑1 (MQ=255) aaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTa < 1:5002017/69‑1 (MQ=255) aaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTa < 1:2173296/69‑1 (MQ=255) aaTATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTa < 1:2836870/69‑1 (MQ=255) atatCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTa < 1:2944132/68‑1 (MQ=255) cGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGttt < 1:806381/54‑1 (MQ=255) gCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGaa > 1:4096956/1‑56 (MQ=255) gCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGaa > 1:214140/1‑56 (MQ=255) gCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGaa > 1:2104237/1‑56 (MQ=255) aCGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAatat > 1:4489365/1‑70 (MQ=255) aCGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAatat > 1:2798351/1‑70 (MQ=255) aCGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAatat > 1:180393/1‑70 (MQ=255) aCGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAata > 1:1286693/1‑69 (MQ=255) cGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAatat < 1:4796360/69‑1 (MQ=255) cGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAatat < 1:4369536/69‑1 (MQ=255) cGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAatat < 1:2707369/69‑1 (MQ=255) cGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAatat < 1:3494135/69‑1 (MQ=255) cGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAatat < 1:1334944/69‑1 (MQ=255) gACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAAtattat < 1:833661/71‑1 (MQ=255) aCCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAAtatta > 1:4756495/1‑69 (MQ=255) aCCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAAtatt > 1:2889349/1‑68 (MQ=255) aCCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATg < 1:5041554/71‑1 (MQ=255) aCCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATg < 1:2128350/71‑1 (MQ=255) ccGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATg < 1:4307304/70‑1 (MQ=255) ccGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGa < 1:4678310/71‑1 (MQ=255) cGTTATCATCTGATCCGTAACCGTAAGATGTCAAACATAAt < 1:1269892/41‑1 (MQ=255) cGTTATCATCTGATCCGTAACCGTAAGATGTCAAACATAAt < 1:4749838/41‑1 (MQ=255) cGTTATCATCTGATCCGTAACCGTAAGATGTCAAACATAAt < 1:1484401/41‑1 (MQ=255) cGTTATCATCTGATCCGTAACCGTAAGATGTCAAACATAAt < 1:1371060/41‑1 (MQ=255) cGTTATCATCTGATCCGTAACCGTAAGATGTCAAACATAAt < 1:3788311/41‑1 (MQ=255) cGTTATCATCTGATCCGTAACCGTAAGATGTCAAACATAAt < 1:2849072/41‑1 (MQ=255) gTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATg < 1:1487996/49‑1 (MQ=255) gTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATg < 1:511633/49‑1 (MQ=255) gTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATg < 1:2955572/49‑1 (MQ=255) ttATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATg > 1:3015041/1‑71 (MQ=255) ttATCATCTGATCCGTAACCGTAAGATGTCAAACATAATg > 1:2533488/1‑40 (MQ=255) ttATCATCTGATCCGTAACCGTAAGATGTCAAACATAATGt > 1:3826145/1‑41 (MQ=255) ttATCATCTGATCCGTAACCGTAAGATGTCAAACATAATGt > 1:5081301/1‑41 (MQ=255) ttATCATCTGATCCGTAACCGTAAGATGTCAAACATAATGt > 1:4978925/1‑41 (MQ=255) tcTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGt < 1:257158/45‑1 (MQ=255) tcTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGt < 1:2587711/45‑1 (MQ=255) tcTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGt < 1:758011/45‑1 (MQ=255) tcTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGt < 1:4512541/45‑1 (MQ=255) tcTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGt < 1:4960625/45‑1 (MQ=255) tcTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGt < 1:522069/45‑1 (MQ=255) aTCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCgcgg < 1:468510/68‑1 (MQ=255) aTCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCgcgg < 1:2773594/68‑1 (MQ=255) aTCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCgcgg < 1:1785326/68‑1 (MQ=255) aTCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGt > 1:2753397/1‑71 (MQ=255) aTCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGt > 1:1327652/1‑71 (MQ=255) cgtaaccgtaaGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTa < 1:4443955/70‑1 (MQ=255) taaccgtaaGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATc > 1:2284309/1‑71 (MQ=255) accgtaaGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATCGt < 1:288605/71‑1 (MQ=255) accgtaaGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATCGt > 1:3113821/1‑71 (MQ=255) taaGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATg < 1:321489/46‑1 (MQ=255) taaGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATg < 1:63456/46‑1 (MQ=255) taaGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATg < 1:3658255/46‑1 (MQ=255) taaGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATg < 1:4546517/46‑1 (MQ=255) taaGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATg < 1:4296546/46‑1 (MQ=255) aGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATCGTCTGgat > 1:1415535/1‑71 (MQ=255) gTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATCGTCTGGatgatg > 1:2043826/1‑71 (MQ=255) gTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATCGTCTGGatgatg > 1:2608005/1‑71 (MQ=255) gTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATCGTCTGGatgatg > 1:2333438/1‑71 (MQ=255) aGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATCGTCTGGATGATGTTa < 1:3187023/71‑1 (MQ=255) | TACCTGCATCAGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCAGACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATCGTCTGGATGATGTTA > Exported/1755887‑1756022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |