| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,829,982 | A→T | 9.8% | G186G (GGA→GGT) | pphB → | serine/threonine‑specific protein phosphatase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,829,982 | 0 | A | T | 9.8% | 138.9 / 7.2 | 51 | G186G (GGA→GGT) | pphB | serine/threonine‑specific protein phosphatase 2 |
| Reads supporting (aligned to +/- strand): ref base A (23/23); new base T (2/3); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
TGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAG > Exported/1829921‑1830050 | tgCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATatgat < 1:1881617/70‑1 (MQ=255) cAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATatga > 1:2737515/1‑67 (MQ=255) aaaTCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGa < 1:694746/57‑1 (MQ=255) aaaTCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGa < 1:1745054/57‑1 (MQ=255) aaaTCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGa < 1:4776085/57‑1 (MQ=255) aaaTCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATATTATATTTGGa < 1:4887738/57‑1 (MQ=255) aaTCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGAt < 1:3629464/71‑1 (MQ=255) aaTCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGAt < 1:4960159/71‑1 (MQ=255) aTCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACa > 1:3836270/1‑57 (MQ=255) aTCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGAt > 1:1162457/1‑70 (MQ=255) aTCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGAt > 1:541791/1‑70 (MQ=255) aTCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGAt > 1:2911345/1‑70 (MQ=255) aTCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGAt > 1:4116313/1‑70 (MQ=255) gCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACa < 1:4830914/71‑1 (MQ=255) gCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACa > 1:4645883/1‑71 (MQ=255) aTGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAg < 1:5144927/71‑1 (MQ=255) tgCGAATTACAAAAAATAAACGGCGCCGACTTCTTTATATTTGGTCATATGATGt < 1:3375736/54‑1 (MQ=255) agCGAATTACAAAAAATAAACGGCGCCGACTTCTTTATATTTGGTCATATGATGt < 1:3675546/54‑1 (MQ=255) agCGAATTACAAAAAATAAACGGCGCCGACTTCTTTATATTTGGTCATATGATGt < 1:3669608/54‑1 (MQ=255) ttACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGAt < 1:3184544/54‑1 (MQ=255) acaacaAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGc > 1:4251514/1‑69 (MQ=255) acaacaAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCt > 1:3577362/1‑70 (MQ=255) acaacaAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCt < 1:5162578/70‑1 (MQ=255) caaATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATaa > 1:4294411/1‑50 (MQ=255) caaATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATaa > 1:1471539/1‑50 (MQ=255) caaATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCa < 1:488673/71‑1 (MQ=255) tAAACGGCGCTGATTATTTTATATTTGGACATTTGATGTTTGATAACATTCAGACGTTCGCTAACCAGAtt < 1:3359698/71‑1 (MQ=255) tAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGAtt < 1:2213249/71‑1 (MQ=255) aaaCGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGAtt > 1:3589054/1‑70 (MQ=255) aaaCGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGAtt > 1:4654079/1‑70 (MQ=255) gATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATAc > 1:4235594/1‑71 (MQ=255) ttattTTATATTTGGACATATGATGTTTGATAACa < 1:104626/35‑1 (MQ=255) ttattTTATATTTGGACATATGATGTTTGATAACa < 1:68396/35‑1 (MQ=255) ttattTTATATTTGGACATATGATGTTTGATAACa < 1:3650677/35‑1 (MQ=255) ttattTTATATTTGGACATATGATGTTTGATAACa < 1:2915724/35‑1 (MQ=255) ttattTTATATTTGGACATATGATGTTTGATAACa < 1:4259567/35‑1 (MQ=255) ttattTTATATTTGGACATATGATGTTTGATAACa < 1:350455/35‑1 (MQ=255) ttattTTATATTTGGACATATGATGTTTGATAACATTCa > 1:2755519/1‑39 (MQ=255) tattTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTc > 1:1966735/1‑45 (MQ=255) tattTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTc > 1:2067526/1‑45 (MQ=255) tattTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTc > 1:97850/1‑45 (MQ=255) attTTATATTTGGACATATGATGTTTGATAACATTCa > 1:2368410/1‑37 (MQ=255) attTTATATTTGGACATATGATGTTTGATAACATTCa > 1:2831512/1‑37 (MQ=255) attTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCgg < 1:9147/70‑1 (MQ=255) ttATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATc < 1:4831225/70‑1 (MQ=255) ttATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATc < 1:518242/70‑1 (MQ=255) tataTTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGAttt < 1:1720555/53‑1 (MQ=255) tataTTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATc < 1:4898284/69‑1 (MQ=255) tatTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCg < 1:1842496/71‑1 (MQ=255) tGGTCATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATAGATACCGGATCGCCGaa > 1:2290604/1‑69 (MQ=255) tGGTCATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATAGATACCGGATCGCCGaa > 1:3768535/1‑69 (MQ=255) ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACa < 1:4281803/70‑1 (MQ=255) ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACa > 1:2696899/1‑70 (MQ=255) ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAg > 1:4865658/1‑71 (MQ=255) ggACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAg > 1:3661351/1‑71 (MQ=255) | TGCAGAAATCGCTTAATGGCGAGTTACAACAAATAAACGGCGCTGATTATTTTATATTTGGACATATGATGTTTGATAACATTCAGACGTTCGCTAACCAGATTTATATTGATACCGGATCGCCGAACAG > Exported/1829921‑1830050 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |