BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,876,422	A→G	5.5%	G334G (GGT→GGC)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,876,422	0	A	G	5.5%	491.5 / 12.8	163	G334G (GGT→GGC)	eno	enolase
Reads supporting (aligned to +/- strand): ref base A (75/79); new base G (7/2); total (82/81)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 163 total aligned reads displayed.

ATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAG  >  Exported/1876357‑1876484

aTTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCtt                                                            <  1:507784/70‑1 (MQ=255)

    cAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                        <  1:989675/70‑1 (MQ=255)

           agTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCg                                                       <  1:1080757/64‑1 (MQ=255)

           agTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCg                                                       <  1:3957510/64‑1 (MQ=255)

             tttCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCttt                                                           <  1:2262260/58‑1 (MQ=255)

             tttCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCttt                                                           <  1:2865356/58‑1 (MQ=255)

             tttCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCttt                                                           <  1:4757376/58‑1 (MQ=255)

             tttCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCttt                                                           <  1:3024429/58‑1 (MQ=255)

                 ggTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                           <  1:1583553/70‑1 (MQ=255)

                     agagAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                           <  1:4624269/66‑1 (MQ=255)

                     agagAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                           <  1:2877634/66‑1 (MQ=255)

                     agagAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                           <  1:880171/66‑1 (MQ=255)

                       agaACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAg                                         >  1:4981690/1‑66 (MQ=255)

                       agaACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAg                                         >  1:2001127/1‑66 (MQ=255)

                       agaACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCt                                    >  1:2468298/1‑71 (MQ=255)

                         aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                        <  1:997366/49‑1 (MQ=255)

                         aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                        <  1:689319/49‑1 (MQ=255)

                         aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                        <  1:279795/49‑1 (MQ=255)

                         aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                        <  1:91402/49‑1 (MQ=255)

                         aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAg                                         >  1:4603730/1‑64 (MQ=255)

                           ccGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                >  1:4944100/1‑71 (MQ=255)

                           ccGATCTGGTTGAATTTGATAAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                >  1:2914062/1‑71 (MQ=255)

                            cGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgtg                               >  1:558299/1‑71 (MQ=255)

                              aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCttt                                            <  1:3816641/56‑1 (MQ=255)

                              aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCttt                                            <  1:2629520/56‑1 (MQ=255)

                              aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAgg                                        <  1:3222279/60‑1 (MQ=255)

                               tCTGGTTTAATTTGATCAGTATGGAGTTAGCGATACCttt                                                           <  1:4259852/40‑1 (MQ=38)

                                cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                        <  1:800735/42‑1 (MQ=255)

                                cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                        <  1:3775632/42‑1 (MQ=255)

                                cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                          >  1:1802947/1‑56 (MQ=255)

                                cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                          >  1:185443/1‑56 (MQ=255)

                                cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                          >  1:3728913/1‑56 (MQ=255)

                                cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                          >  1:1106369/1‑56 (MQ=255)

                                cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACATTCTTTCa                                          >  1:1520446/1‑56 (MQ=255)

                                  ggTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                      >  1:2199071/1‑42 (MQ=255)

                                  ggTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                      >  1:3456690/1‑42 (MQ=255)

                                  ggTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                      >  1:849114/1‑42 (MQ=255)

                                  ggTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                      >  1:4481263/1‑42 (MQ=255)

                                   gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCg                                                       >  1:2039598/1‑40 (MQ=255)

                                   gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                      >  1:3710495/1‑41 (MQ=255)

                                   gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                      >  1:1324299/1‑41 (MQ=255)

                                   gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                      >  1:3736611/1‑41 (MQ=255)

                                   gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                      >  1:2089969/1‑41 (MQ=255)

                                   gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATAACTTCTTTCAGGATCTTg                                  >  1:4029378/1‑61 (MQ=255)

                                      gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                      <  1:3063040/70‑1 (MQ=255)

                                      gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                      <  1:3045234/70‑1 (MQ=255)

                                      gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                      <  1:2848929/70‑1 (MQ=255)

                                      gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                      <  1:4326913/70‑1 (MQ=255)

                                       aaTTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                        <  1:1195910/35‑1 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                      <  1:1727563/67‑1 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                      <  1:40257/67‑1 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                      <  1:3598872/67‑1 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                    >  1:627520/1‑69 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                    >  1:693836/1‑69 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                    >  1:4871698/1‑69 (MQ=255)

                                           tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                    >  1:1075451/1‑67 (MQ=255)

                                           tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                    >  1:3665126/1‑67 (MQ=255)

                                           tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                    >  1:5054819/1‑67 (MQ=255)

                                           tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                    >  1:1686253/1‑67 (MQ=255)

                                           tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                    >  1:1150991/1‑67 (MQ=255)

                                           tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAg                 <  1:1302349/70‑1 (MQ=255)

                                           tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAg                 <  1:1774524/70‑1 (MQ=255)

                                            gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTTGTTACGa                     <  1:3168249/65‑1 (MQ=255)

                                            gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                     <  1:492896/65‑1 (MQ=255)

                                            gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                     <  1:4529042/65‑1 (MQ=255)

                                            gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                     <  1:2690427/65‑1 (MQ=255)

                                            gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                     <  1:4309621/65‑1 (MQ=255)

                                              tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg             <  1:4449395/71‑1 (MQ=255)

                                              tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg             <  1:3831546/71‑1 (MQ=255)

                                              tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg             <  1:353336/71‑1 (MQ=255)

                                              tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg             <  1:3478484/71‑1 (MQ=255)

                                               cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                >  1:3877099/1‑51 (MQ=255)

                                               cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                >  1:3222409/1‑51 (MQ=255)

                                               cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                >  1:4845378/1‑51 (MQ=255)

                                               cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                >  1:3745068/1‑51 (MQ=255)

                                               cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt            >  1:2014484/1‑71 (MQ=255)

                                               cAGGATGGAGTTAGCGATACCTTTTTAGATACCTTCTTTCAGGATCTTGgt                                >  1:2605396/1‑51 (MQ=255)

                                                aGGATCGAGTTGGCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAGgtcgtc           <  1:4901062/71‑1 (MQ=255)

                                                  gATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAAAAGGTCGTCac         <  1:4469081/71‑1 (MQ=255)

                                                   aTGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                      <  1:1798510/57‑1 (MQ=255)

                                                    tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATc                                     >  1:1876612/1‑41 (MQ=255)

                                                    tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATc                                     >  1:2736122/1‑41 (MQ=255)

                                                    tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATc                                     >  1:4928165/1‑41 (MQ=255)

                                                     ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc              <  1:3374327/63‑1 (MQ=255)

                                                     ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc              <  1:4488300/63‑1 (MQ=255)

                                                       aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTg                                  >  1:1959907/1‑41 (MQ=255)

                                                       aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt            >  1:5025373/1‑63 (MQ=255)

                                                       aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt            >  1:1185690/1‑63 (MQ=255)

                                                       aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt            >  1:3847053/1‑63 (MQ=255)

                                                       aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt            >  1:3989041/1‑63 (MQ=255)

                                                       aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg             >  1:4222619/1‑62 (MQ=255)

                                                       aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgacgt            >  1:968472/1‑63 (MQ=255)

                                                        gTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCaccaac     >  1:1411472/1‑69 (MQ=255)

                                                         ttAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAg  <  1:1934896/71‑1 (MQ=255)

                                                           ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                >  1:3174283/2‑55 (MQ=255)

                                                           ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                >  1:5188816/2‑55 (MQ=255)

                                                           ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                >  1:18579/2‑55 (MQ=255)

                                                           ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                >  1:2686370/2‑55 (MQ=255)

                                                           aGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                      <  1:1145920/49‑1 (MQ=255)

                                                            gCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGGCACCAACCAg  <  1:1321393/68‑1 (MQ=255)

ATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAG  >  Exported/1876357‑1876484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`