BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,876,428	T→C	5.5%	E332E (GAA→GAG) ‡	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,876,428	0	T	C	5.5%	497.8 / 13.1	164	E332E (GAA→GAG) ‡	eno	enolase
Reads supporting (aligned to +/- strand): ref base T (82/73); new base C (7/2); total (89/75)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

Only 100 of 165 total aligned reads displayed.

CAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTT  >  Exported/1876361‑1876493

cAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:989675/70‑1 (MQ=255)

   ccAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATa                                                             >  1:1544066/1‑71 (MQ=255)

     agagTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATa                                                             >  1:4930577/1‑69 (MQ=255)

       agTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCg                                                                <  1:1080757/64‑1 (MQ=255)

       agTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCg                                                                <  1:3957510/64‑1 (MQ=255)

             ggTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:480855/70‑1 (MQ=255)

             ggTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:1583553/70‑1 (MQ=255)

                 agagAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:880171/66‑1 (MQ=255)

                 agagAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:4624269/66‑1 (MQ=255)

                 agagAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:2877634/66‑1 (MQ=255)

                   agaACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAg                                                  >  1:4981690/1‑66 (MQ=255)

                   agaACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCt                                             >  1:2468298/1‑71 (MQ=255)

                     aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:689319/49‑1 (MQ=255)

                     aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:5199143/49‑1 (MQ=255)

                     aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:91402/49‑1 (MQ=255)

                     aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:997366/49‑1 (MQ=255)

                     aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:279795/49‑1 (MQ=255)

                        cGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgtg                                        >  1:558299/1‑71 (MQ=255)

                          aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCttt                                                     <  1:3816641/56‑1 (MQ=255)

                          aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCttt                                                     <  1:2629520/56‑1 (MQ=255)

                          aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAgg                                                 <  1:3222279/60‑1 (MQ=255)

                          aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAgg                                                 <  1:5117033/60‑1 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:3775632/42‑1 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:3532076/42‑1 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:1921805/42‑1 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:4557170/42‑1 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:800735/42‑1 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:3057032/42‑1 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                                   >  1:1802947/1‑56 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                                   >  1:1106369/1‑56 (MQ=255)

                              ggTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:2894631/1‑42 (MQ=255)

                              ggTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:849114/1‑42 (MQ=255)

                              ggTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:3456690/1‑42 (MQ=255)

                               gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCg                                                                >  1:2039598/1‑40 (MQ=255)

                               gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:2072748/1‑41 (MQ=255)

                               gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:1738719/1‑41 (MQ=255)

                               gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:1324299/1‑41 (MQ=255)

                               gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:3736611/1‑41 (MQ=255)

                               gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTa                                 >  1:3280688/1‑71 (MQ=255)

                                  gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:2848929/70‑1 (MQ=255)

                                  gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:3555084/70‑1 (MQ=255)

                                  gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:3063040/70‑1 (MQ=255)

                                  gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:4326913/70‑1 (MQ=255)

                                   aaTTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:1195910/35‑1 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:1615638/67‑1 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:1796853/67‑1 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:40257/67‑1 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:198063/67‑1 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:1727563/67‑1 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:4871698/1‑69 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:5027547/1‑69 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:3720977/1‑69 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:2097176/1‑69 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:693836/1‑69 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTAc                                >  1:3862310/1‑64 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:1150991/1‑67 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:1075451/1‑67 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:3706650/1‑67 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:3665126/1‑67 (MQ=255)

                                        gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTTGTTACGa                              <  1:3168249/65‑1 (MQ=255)

                                        gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              <  1:3742546/65‑1 (MQ=255)

                                        gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              <  1:492896/65‑1 (MQ=255)

                                        gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              <  1:4529042/65‑1 (MQ=255)

                                        gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              <  1:1320598/65‑1 (MQ=255)

                                        gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              <  1:2690427/65‑1 (MQ=255)

                                          tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg                      <  1:3831546/71‑1 (MQ=255)

                                           cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                         >  1:4472023/1‑51 (MQ=255)

                                           cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                         >  1:2928097/1‑51 (MQ=255)

                                           cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                         >  1:3877099/1‑51 (MQ=255)

                                           cAGGATGGAGTTAGCGATACCTTTTTAGATACCTTCTTTCAGGATCTTGgt                                         >  1:2605396/1‑51 (MQ=255)

                                            aGGATCGAGTTGGCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAGgtcgtc                    <  1:4901062/71‑1 (MQ=255)

                                              gATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAAAAGGTCGTCac                  <  1:4469081/71‑1 (MQ=255)

                                                tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATc                                              >  1:648017/1‑41 (MQ=255)

                                                tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATc                                              >  1:422539/1‑41 (MQ=255)

                                                tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATc                                              >  1:2736122/1‑41 (MQ=255)

                                                tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATc                                              >  1:1876612/1‑41 (MQ=255)

                                                tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCacca                <  1:4322975/71‑1 (MQ=255)

                                                tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCacca                <  1:4911037/71‑1 (MQ=255)

                                                 ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc                       <  1:1833948/63‑1 (MQ=255)

                                                 ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc                       <  1:4488300/63‑1 (MQ=255)

                                                 ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc                       <  1:3374327/63‑1 (MQ=255)

                                                   aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTg                                           >  1:1959907/1‑41 (MQ=255)

                                                   aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                     >  1:5025373/1‑63 (MQ=255)

                                                   aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                     >  1:1185690/1‑63 (MQ=255)

                                                   aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                     >  1:3989041/1‑63 (MQ=255)

                                                   aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgacgt                     >  1:968472/1‑63 (MQ=255)

                                                    gTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCaccaac              >  1:1411472/1‑69 (MQ=255)

                                                     ttAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAg           <  1:4148953/71‑1 (MQ=255)

                                                     ttAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAg           <  1:2013951/71‑1 (MQ=255)

                                                       ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                         >  1:3174283/2‑55 (MQ=255)

                                                       ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                         >  1:18579/2‑55 (MQ=255)

                                                       ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                         >  1:2686370/2‑55 (MQ=255)

                                                       aGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:1145920/49‑1 (MQ=255)

                                                              ccTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGAtttt  <  1:1329090/71‑1 (MQ=255)

                                                               cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                   >  1:473569/1‑53 (MQ=255)

                                                               cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                   >  1:3494194/1‑53 (MQ=255)

                                                               cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                   >  1:4107189/1‑53 (MQ=255)

                                                               cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                   >  1:2857168/1‑53 (MQ=255)

                                                               cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                   >  1:443854/1‑53 (MQ=255)

                                                                  tttCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGAtttt  <  1:3780833/67‑1 (MQ=255)

CAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTT  >  Exported/1876361‑1876493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`