BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,876,430	C→G	5.7%	E332Q (GAA→CAA) ‡	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,876,430	0	C	G	5.7%	515.6 / 13.2	157	E332Q (GAA→CAA) ‡	eno	enolase
Reads supporting (aligned to +/- strand): ref base C (89/58); new base G (7/2); total (97/60)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 173 total aligned reads displayed.

CAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTT  >  Exported/1876361‑1876493

cAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:989675/70‑1 (MQ=255)

     agagTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATa                                                             >  1:4930577/1‑69 (MQ=255)

       agTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCg                                                                <  1:1080757/64‑1 (MQ=255)

           tCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCttt                                                     >  1:947790/1‑71 (MQ=255)

             ggTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:480855/70‑1 (MQ=255)

             ggTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:1583553/70‑1 (MQ=255)

                 agagAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:880171/66‑1 (MQ=255)

                   agaACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAg                                                  >  1:4981690/1‑66 (MQ=255)

                   agaACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAg                                                  >  1:2001127/1‑66 (MQ=255)

                     aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:997366/49‑1 (MQ=255)

                     aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:91402/49‑1 (MQ=255)

                     aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:689319/49‑1 (MQ=255)

                     aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:5199143/49‑1 (MQ=255)

                     aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:285375/49‑1 (MQ=255)

                     aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:279795/49‑1 (MQ=255)

                     aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAg                                                  >  1:4603730/1‑64 (MQ=255)

                       ccGATCTGGTTGAATTTGATAAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                         >  1:2914062/1‑71 (MQ=255)

                        cGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgtg                                        >  1:558299/1‑71 (MQ=255)

                          aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCttt                                                     <  1:2629520/56‑1 (MQ=255)

                          aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAgg                                                 <  1:3222279/60‑1 (MQ=255)

                          aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAgg                                                 <  1:5117033/60‑1 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:1921805/42‑1 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:3532076/42‑1 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:4557170/42‑1 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:794516/42‑1 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:3775632/42‑1 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                                   >  1:3106115/1‑56 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                                   >  1:1106369/1‑56 (MQ=255)

                            cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACATTCTTTCa                                                   >  1:1520446/1‑56 (MQ=255)

                            cTGGTCGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                                   >  1:2612240/1‑56 (MQ=255)

                              ggTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:2894631/1‑42 (MQ=255)

                              ggTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:3456690/1‑42 (MQ=255)

                              ggTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:4481263/1‑42 (MQ=255)

                               gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCg                                                                >  1:2039598/1‑40 (MQ=255)

                               gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:3736611/1‑41 (MQ=255)

                               gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:2089969/1‑41 (MQ=255)

                               gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:1738719/1‑41 (MQ=255)

                               gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:1324299/1‑41 (MQ=255)

                               gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTa                                 >  1:3280688/1‑71 (MQ=255)

                               gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATAACTTCTTTCAGGATCTTg                                           >  1:4029378/1‑61 (MQ=255)

                                  gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:3045234/70‑1 (MQ=255)

                                  gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:3063040/70‑1 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:1867709/46‑1 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:3598872/67‑1 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:1409174/67‑1 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:3720977/1‑69 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:4687824/1‑69 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:4871698/1‑69 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:5027547/1‑69 (MQ=255)

                                     tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:2097176/1‑69 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTAc                                >  1:3862310/1‑64 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:1686253/1‑67 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:2695796/1‑67 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:3706650/1‑67 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:4491081/1‑67 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:1150991/1‑67 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:1075451/1‑67 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAgg                         >  1:4372961/1‑71 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAg                          <  1:1302349/70‑1 (MQ=255)

                                       tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAg                          <  1:1774524/70‑1 (MQ=255)

                                        gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              <  1:4012794/65‑1 (MQ=255)

                                          tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg                      <  1:353336/71‑1 (MQ=255)

                                          tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg                      <  1:840978/71‑1 (MQ=255)

                                          tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg                      <  1:4449395/71‑1 (MQ=255)

                                           cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                         >  1:3745068/1‑51 (MQ=255)

                                             ggATCGAGTTGGCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAGgtcgtc                    <  1:2755873/70‑1 (MQ=255)

                                              gATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAAAAGGTCGTCac                  <  1:4469081/71‑1 (MQ=255)

                                               aTGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:1798510/57‑1 (MQ=255)

                                                tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATc                                              >  1:422539/1‑41 (MQ=255)

                                                tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATc                                              >  1:648017/1‑41 (MQ=255)

                                                tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATc                                              >  1:4928165/1‑41 (MQ=255)

                                                tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCacca                <  1:4322975/71‑1 (MQ=255)

                                                tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCacca                <  1:4911037/71‑1 (MQ=255)

                                                 ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc                       <  1:1833948/63‑1 (MQ=255)

                                                 ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc                       <  1:3374327/63‑1 (MQ=255)

                                                   aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTg                                           >  1:1959907/1‑41 (MQ=255)

                                                   aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                     >  1:5025373/1‑63 (MQ=255)

                                                   aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                     >  1:3989041/1‑63 (MQ=255)

                                                   aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                     >  1:1147867/1‑63 (MQ=255)

                                                   aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                     >  1:1185690/1‑63 (MQ=255)

                                                   aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                     >  1:3847053/1‑63 (MQ=255)

                                                   aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg                      >  1:4222619/1‑62 (MQ=255)

                                                     ttAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAg           <  1:3896445/71‑1 (MQ=255)

                                                     ttAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAg           <  1:4148953/71‑1 (MQ=255)

                                                     ttAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAg           <  1:2013951/71‑1 (MQ=255)

                                                       ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                         >  1:18579/2‑55 (MQ=255)

                                                       ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                         >  1:3174283/2‑55 (MQ=255)

                                                       ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                         >  1:2686370/2‑55 (MQ=255)

                                                       aGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              <  1:381944/50‑1 (MQ=255)

                                                        gCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGGCACCAACCAg           <  1:1321393/68‑1 (MQ=255)

                                                              ccTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGAtttt  <  1:2857281/71‑1 (MQ=255)

                                                               cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                   >  1:1587478/1‑53 (MQ=255)

                                                               cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                   >  1:3494194/1‑53 (MQ=255)

                                                                  tttCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGAtttt  <  1:3780833/67‑1 (MQ=255)

                                                                    tCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:224862/1‑38 (MQ=255)

                                                                    tCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:794740/1‑38 (MQ=255)

                                                                    tCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:2585999/1‑38 (MQ=255)

                                                                    tCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:4809503/1‑38 (MQ=255)

                                                                    tCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:3701464/1‑38 (MQ=255)

                                                                    tCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              >  1:488313/1‑37 (MQ=255)

CAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTT  >  Exported/1876361‑1876493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`