BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,876,434	A→G	6.4%	G330G (GGT→GGC)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,876,434	0	A	G	6.4%	414.1 / 13.3	141	G330G (GGT→GGC)	eno	enolase
Reads supporting (aligned to +/- strand): ref base A (75/57); new base G (7/2); total (82/59)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 143 total aligned reads displayed.

CCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAG  >  Exported/1876364‑1876502

ccAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATa                                                                      >  1:1544066/1‑71 (MQ=255)

  agagTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATa                                                                      >  1:4930577/1‑69 (MQ=255)

        tCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCttt                                                              >  1:947790/1‑71 (MQ=255)

          ggTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                             <  1:480855/70‑1 (MQ=255)

              agagAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                             <  1:2877634/66‑1 (MQ=255)

              agagAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                             <  1:880171/66‑1 (MQ=255)

                agaACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAg                                                           >  1:5022301/1‑66 (MQ=255)

                agaACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAg                                                           >  1:4981690/1‑66 (MQ=255)

                agaACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCt                                                      >  1:2468298/1‑71 (MQ=255)

                  aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAg                                                           >  1:4603730/1‑64 (MQ=255)

                    ccGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                                  >  1:1092509/1‑71 (MQ=255)

                     cGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgtg                                                 >  1:558299/1‑71 (MQ=255)

                       aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCttt                                                              <  1:3816641/56‑1 (MQ=255)

                       aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAgg                                                          <  1:3222279/60‑1 (MQ=255)

                         cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                                            >  1:1802947/1‑56 (MQ=255)

                         cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                                            >  1:3106115/1‑56 (MQ=255)

                         cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                                            >  1:1106369/1‑56 (MQ=255)

                         cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACATTCTTTCa                                                            >  1:1520446/1‑56 (MQ=255)

                         cTGGTCGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                                            >  1:2612240/1‑56 (MQ=255)

                            gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATAACTTCTTTCAGGATCTTg                                                    >  1:4029378/1‑61 (MQ=255)

                               gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                        <  1:3555084/70‑1 (MQ=255)

                               gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                        <  1:3045234/70‑1 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                             <  1:1867709/46‑1 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                        <  1:40257/67‑1 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                        <  1:3414944/67‑1 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                        <  1:3598872/67‑1 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                        <  1:1727563/67‑1 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                        <  1:1615638/67‑1 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                        <  1:198063/67‑1 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                        <  1:1796853/67‑1 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                        <  1:1409174/67‑1 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:693836/1‑69 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:627520/1‑69 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:3720977/1‑69 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:5027547/1‑69 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:4871698/1‑69 (MQ=255)

                                  tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:4687824/1‑69 (MQ=255)

                                    tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTAc                                         >  1:3862310/1‑64 (MQ=255)

                                    tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:1150991/1‑67 (MQ=255)

                                    tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:3706650/1‑67 (MQ=255)

                                    tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:3665126/1‑67 (MQ=255)

                                    tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:1075451/1‑67 (MQ=255)

                                    tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:5054819/1‑67 (MQ=255)

                                    tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAgg                                  >  1:4372961/1‑71 (MQ=255)

                                    tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAg                                   <  1:1774524/70‑1 (MQ=255)

                                    tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAg                                   <  1:1302349/70‑1 (MQ=255)

                                     gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTTGTTACGa                                       <  1:3168249/65‑1 (MQ=255)

                                     gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                                       <  1:4529042/65‑1 (MQ=255)

                                     gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                                       <  1:3742546/65‑1 (MQ=255)

                                     gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                                       <  1:2690427/65‑1 (MQ=255)

                                     gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                                       <  1:492896/65‑1 (MQ=255)

                                     gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                                       <  1:4309621/65‑1 (MQ=255)

                                       tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg                               <  1:4449395/71‑1 (MQ=255)

                                       tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg                               <  1:840978/71‑1 (MQ=255)

                                        cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                                  >  1:2928097/1‑51 (MQ=255)

                                        cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                                  >  1:4845378/1‑51 (MQ=255)

                                        cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                                  >  1:3745068/1‑51 (MQ=255)

                                        cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                                  >  1:4472023/1‑51 (MQ=255)

                                        cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                                  >  1:3222409/1‑51 (MQ=255)

                                        cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                              >  1:2014484/1‑71 (MQ=255)

                                        cAGGATGGAGTTAGCGATACCTTTTTAGATACCTTCTTTCAGGATCTTGgt                                                  >  1:2605396/1‑51 (MQ=255)

                                         aGGATCGAGTTGGCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAGgtcgtc                             <  1:4901062/71‑1 (MQ=255)

                                          ggATCGAGTTGGCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAGgtcgtc                             <  1:2755873/70‑1 (MQ=255)

                                             tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATc                                                       >  1:2736122/1‑41 (MQ=255)

                                             tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATc                                                       >  1:4928165/1‑41 (MQ=255)

                                             tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCacca                         <  1:4322975/71‑1 (MQ=255)

                                              ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc                                <  1:1833948/63‑1 (MQ=255)

                                              ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc                                <  1:3374327/63‑1 (MQ=255)

                                              ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc                                <  1:4488300/63‑1 (MQ=255)

                                                aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTg                                                    >  1:1959907/1‑41 (MQ=255)

                                                aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                              >  1:3847053/1‑63 (MQ=255)

                                                aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                              >  1:3989041/1‑63 (MQ=255)

                                                aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                              >  1:1147867/1‑63 (MQ=255)

                                                aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                              >  1:1185690/1‑63 (MQ=255)

                                                aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg                               >  1:4222619/1‑62 (MQ=255)

                                                aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgacgt                              >  1:968472/1‑63 (MQ=255)

                                                  ttAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAg                    <  1:3896445/71‑1 (MQ=255)

                                                  ttAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAg                    <  1:2013951/71‑1 (MQ=255)

                                                  ttAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAg                    <  1:1934896/71‑1 (MQ=255)

                                                    ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                                  >  1:3215041/2‑55 (MQ=255)

                                                    ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                                  >  1:5188816/2‑55 (MQ=255)

                                                    ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                                  >  1:2686370/2‑55 (MQ=255)

                                                    ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                                  >  1:18579/2‑55 (MQ=255)

                                                    aGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                                       <  1:381944/50‑1 (MQ=255)

                                                     gCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGGCACCAACCAg                    <  1:1321393/68‑1 (MQ=255)

                                                           ccTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGAtttt           <  1:1329090/71‑1 (MQ=255)

                                                           ccTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGAtttt           <  1:2857281/71‑1 (MQ=255)

                                                            cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                            >  1:1587478/1‑53 (MQ=255)

                                                            cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                            >  1:650838/1‑53 (MQ=255)

                                                            cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                            >  1:473569/1‑53 (MQ=255)

                                                            cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                            >  1:4107189/1‑53 (MQ=255)

                                                            cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                            >  1:2857168/1‑53 (MQ=255)

                                                            cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                            >  1:3494194/1‑53 (MQ=255)

                                                            cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                            >  1:443854/1‑53 (MQ=255)

                                                               tttCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGAtttt           <  1:3780833/67‑1 (MQ=255)

                                                                 tCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:4168708/1‑38 (MQ=255)

                                                                 tCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:3521564/1‑38 (MQ=255)

                                                                 tCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:2585999/1‑38 (MQ=255)

                                                                 tCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                      >  1:224862/1‑38 (MQ=255)

                                                                 tCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                                       >  1:488313/1‑37 (MQ=255)

CCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAG  >  Exported/1876364‑1876502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`