Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,876,461 T→G 6.0% V321V (GTA→GTC)  eno ← enolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,876,4610TG 6.0% 415.0 / 18.4 150V321V (GTA→GTC) enoenolase
Reads supporting (aligned to +/- strand):  ref base T (67/74);  new base G (7/2);  total (74/76)
Fisher's exact test for biased strand distribution p-value = 9.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 151 total aligned reads displayed.

GTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTGGTATGCGAAACCGTC  >  Exported/1876392‑1876529
                                                                     |                                                                    
   gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                                                   <  1:2848929/70‑1 (MQ=255)
   gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                                                   <  1:3045234/70‑1 (MQ=255)
   gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                                                   <  1:3555084/70‑1 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                                                   <  1:40257/67‑1 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                                                   <  1:198063/67‑1 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                                                   <  1:1727563/67‑1 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                                                   <  1:3414944/67‑1 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                                                   <  1:1615638/67‑1 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                                                   <  1:1409174/67‑1 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                                                   <  1:3598872/67‑1 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:4687824/1‑69 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:4871698/1‑69 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:3720977/1‑69 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:693836/1‑69 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:627520/1‑69 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:4881225/1‑69 (MQ=255)
      tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:2097176/1‑69 (MQ=255)
        tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTAc                                                                    >  1:3862310/1‑64 (MQ=255)
        tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:3665126/1‑67 (MQ=255)
        tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:3706650/1‑67 (MQ=255)
        tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:4491081/1‑67 (MQ=255)
        tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:2695796/1‑67 (MQ=255)
        tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:1075451/1‑67 (MQ=255)
        tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:5054819/1‑67 (MQ=255)
        tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAgg                                                             >  1:4372961/1‑71 (MQ=255)
        tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAg                                                              <  1:1774524/70‑1 (MQ=255)
         gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                                                                  <  1:1320598/65‑1 (MQ=255)
         gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                                                                  <  1:4529042/65‑1 (MQ=255)
         gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                                                                  <  1:4309621/65‑1 (MQ=255)
         gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                                                                  <  1:2690427/65‑1 (MQ=255)
           tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg                                                          <  1:840978/71‑1 (MQ=255)
            cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                                                         >  1:2014484/1‑71 (MQ=255)
             aGGATCGAGTTGGCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAGgtcgtc                                                        <  1:4901062/71‑1 (MQ=255)
               gATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAAAAGGTCGTCac                                                      <  1:4469081/71‑1 (MQ=255)
                aTGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                                                   <  1:1798510/57‑1 (MQ=255)
                 tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCacca                                                    <  1:4322975/71‑1 (MQ=255)
                 tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCacca                                                    <  1:4911037/71‑1 (MQ=255)
                  ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc                                                           <  1:3374327/63‑1 (MQ=255)
                    aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                                                         >  1:1185690/1‑63 (MQ=255)
                    aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                                                         >  1:3847053/1‑63 (MQ=255)
                    aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                                                         >  1:3989041/1‑63 (MQ=255)
                    aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                                                         >  1:1147867/1‑63 (MQ=255)
                    aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                                                         >  1:5025373/1‑63 (MQ=255)
                      ttAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAg                                               <  1:4148953/71‑1 (MQ=255)
                      ttAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAg                                               <  1:2013951/71‑1 (MQ=255)
                      ttAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAg                                               <  1:1934896/71‑1 (MQ=255)
                        ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                                                             >  1:3215041/2‑55 (MQ=255)
                        ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                                                             >  1:3174283/2‑55 (MQ=255)
                        ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                                                             >  1:900608/2‑55 (MQ=255)
                        ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                                                             >  1:18579/2‑55 (MQ=255)
                        ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                                                             >  1:5188816/2‑55 (MQ=255)
                        aGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                                                                   <  1:1145920/49‑1 (MQ=255)
                        aGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                                                                  <  1:381944/50‑1 (MQ=255)
                               ccTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGAtttt                                      <  1:1329090/71‑1 (MQ=255)
                                cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                                                       >  1:2857168/1‑53 (MQ=255)
                                cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                                                       >  1:3494194/1‑53 (MQ=255)
                                cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                                                       >  1:1587478/1‑53 (MQ=255)
                                cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                                                       >  1:443854/1‑53 (MQ=255)
                                   tttCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGAtttt                                      <  1:3780833/67‑1 (MQ=255)
                                     tCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:224862/1‑38 (MQ=255)
                                     tCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                                                                 >  1:4168708/1‑38 (MQ=255)
                                        aTACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAg                             <  1:2556778/71‑1 (MQ=255)
                                           ccTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGaa                           <  1:1303250/70‑1 (MQ=255)
                                            cttcttTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                                                         <  1:3460659/39‑1 (MQ=255)
                                            cttcttTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                                                         <  1:3104870/39‑1 (MQ=255)
                                               cttTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCaccaac                                                  >  1:931023/1‑43 (MQ=255)
                                               cttTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCaccaac                                                  >  1:348236/1‑43 (MQ=255)
                                               cttTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCaccaac                                                  >  1:3109298/1‑43 (MQ=255)
                                               cttTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCaccaac                                                  >  1:286959/1‑43 (MQ=255)
                                               cttTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCaccaac                                                  >  1:544641/1‑43 (MQ=255)
                                               cgatCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTg                      <  1:3526718/68‑1 (MQ=255)
                                               cgatCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTg                      <  1:3950905/68‑1 (MQ=255)
                                                  tCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCacc                                                     <  1:4239525/37‑1 (MQ=255)
                                                  tCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCacc                                                     <  1:1412653/37‑1 (MQ=255)
                                                  tCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCacc                                                     <  1:1627464/37‑1 (MQ=255)
                                                  tCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCacc                                                     <  1:2621632/37‑1 (MQ=255)
                                                  tCAGGATCTTGGTGTTGGTTACGAACAGGACGTCacc                                                     <  1:4790979/37‑1 (MQ=255)
                                                     ggATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTgg                <  1:2140501/71‑1 (MQ=255)
                                                     ggATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTgg                <  1:5072466/71‑1 (MQ=255)
                                                     ggATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTgg                <  1:2410560/71‑1 (MQ=255)
                                                      gATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTgg                <  1:647816/70‑1 (MQ=255)
                                                            ggTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCg                                  >  1:4929880/1‑46 (MQ=255)
                                                            ggTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCg                                  >  1:4023660/1‑46 (MQ=255)
                                                            ggTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCg                                  >  1:4756279/1‑46 (MQ=255)
                                                            ggTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCg                                  >  1:106101/1‑46 (MQ=255)
                                                            ggTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCg                                  >  1:2624552/1‑46 (MQ=255)
                                                            ggTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAg                             >  1:1802731/1‑51 (MQ=255)
                                                            ggTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAg                             >  1:468750/1‑51 (MQ=255)
                                                            ggTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTGGTATg            <  1:3458039/68‑1 (MQ=255)
                                                             gtgtTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTGGTATGCg          >  1:2230574/1‑69 (MQ=255)
                                                             gtgtTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTGGTATGCGa         <  1:1915180/70‑1 (MQ=255)
                                                             gtgtTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTGGTATGCGa         <  1:930187/70‑1 (MQ=255)
                                                             gtgtTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTGGTATGCGa         <  1:3592468/70‑1 (MQ=255)
                                                             gtgtTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTGGTATGCGa         <  1:4271571/70‑1 (MQ=255)
                                                             gtgtTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTGGTATGCGa         <  1:2554026/70‑1 (MQ=255)
                                                                ttGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTGGTATGCGAAAcc     >  1:724803/1‑71 (MQ=255)
                                                                  ggTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTGGTATGCGAACCCGt   >  1:1281860/1‑71 (MQ=255)
                                                                  ggTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTGGTATGCGAAACCGt   >  1:2362090/1‑71 (MQ=255)
                                                                   gTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTGGTATGCGAAACCGTc  >  1:4463348/1‑71 (MQ=255)
                                                                    ttACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTc                   >  1:985756/1‑53 (MQ=255)
                                                                     |                                                                    
GTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTTGTCGCCCAGAACTTTGGTCTGGTATGCGAAACCGTC  >  Exported/1876392‑1876529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑