Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,266,204 T→A 13.9% V80V (GTA→GTT tufB ← protein chain elongation factor EF‑Tu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,266,2040TA13.9% 89.3 / 8.1 36V80V (GTA→GTTtufBprotein chain elongation factor EF‑Tu
Reads supporting (aligned to +/- strand):  ref base T (9/22);  new base A (3/2);  total (12/24)
Fisher's exact test for biased strand distribution p-value = 3.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01

TCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGG  >  Exported/2266137‑2266222
                                                                   |                  
tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc                  >  1:1514593/1‑70 (MQ=35)
tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACg                 >  1:3369030/1‑71 (MQ=34)
tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACg                 >  1:4584516/1‑71 (MQ=34)
tCGCGCCGTCCATCTGCGCAGCACAGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACg                 >  1:2615751/1‑71 (MQ=21)
     ccGTCCATCTGCGCAGCACCGGTGATCATGTTTTCAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGc            >  1:2021762/1‑71 (MQ=21)
      cggccATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg           <  1:1234672/68‑1 (MQ=21)
      cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg           <  1:1024165/71‑1 (MQ=34)
      cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg           <  1:2576480/71‑1 (MQ=34)
      cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg           <  1:3536838/71‑1 (MQ=34)
      cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCg           <  1:5083395/71‑1 (MQ=34)
       gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTTCCCCGGGCAGTCTACGTGTGCGt          <  1:116911/71‑1 (MQ=21)
       gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt          <  1:1005779/71‑1 (MQ=34)
       gTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGt          <  1:758690/71‑1 (MQ=34)
        tCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTa         <  1:4593377/71‑1 (MQ=34)
         ccATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTCAACGTGCGCGTa         >  1:2323996/1‑70 (MQ=2)
         ccATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTCAACGTGCGCGTa         >  1:2374542/1‑70 (MQ=2)
         ccATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTCAACGTGCGCGTAg        >  1:4104371/1‑71 (MQ=2)
          cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAg        <  1:1112507/70‑1 (MQ=35)
          cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAg        <  1:2274659/70‑1 (MQ=35)
          cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt       >  1:4327106/1‑71 (MQ=34)
          cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt       >  1:2603834/1‑71 (MQ=34)
          cATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTCAACGTGAGCGTAGt       <  1:1730082/71‑1 (MQ=2)
          cATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTCAACGTGAGCGTAGt       <  1:4400135/71‑1 (MQ=2)
            tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt       <  1:2374937/69‑1 (MQ=35)
            tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt       <  1:4568808/69‑1 (MQ=35)
            tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt       <  1:1465794/69‑1 (MQ=35)
            tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt       <  1:1075632/69‑1 (MQ=35)
            tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt       <  1:770768/69‑1 (MQ=35)
            tCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt       <  1:88499/69‑1 (MQ=35)
            tCTGCGCAGCACCGGTGATCATGTGTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt       <  1:738858/69‑1 (MQ=25)
             cTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt       <  1:3748934/68‑1 (MQ=35)
              tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTGTACGTGTGCGTAGTGACg   >  1:2382090/1‑71 (MQ=21)
              tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACg   >  1:2796724/1‑71 (MQ=34)
                cgcAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACgg  <  1:3849002/70‑1 (MQ=31)
                cgcAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACgg  <  1:1102974/70‑1 (MQ=31)
                cgcAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACgg  <  1:1077606/70‑1 (MQ=31)
                                                                   |                  
TCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACGG  >  Exported/2266137‑2266222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: