| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,343,092 | C→T | 24.3% | H273H (CAC→CAT) | glnL → | sensory kinase in two‑component regulatory system with GlnG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,343,092 | 0 | C | T | 24.3% | 62.7 / 18.7 | 37 | H273H (CAC→CAT) | glnL | sensory kinase in two‑component regulatory system with GlnG |
| Reads supporting (aligned to +/- strand): ref base C (6/22); new base T (4/5); total (10/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
GGGGCCGGAAGGCGGTGAAATCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCATTCCGCCTCATT > Exported/2343032‑2343159 | ggggCCGGAAGGCGGTGAAATCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAgcgc > 1:410392/1‑70 (MQ=255)ggggCCGGAAGGCGGTGAAATCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAgcgc > 1:2692645/1‑70 (MQ=255) gCCGGAAGGCGGTGAAATCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTAcc < 1:4773836/71‑1 (MQ=255) gCCGGAAGGCGGTGAAATCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTAcc < 1:2132218/71‑1 (MQ=255) aGGCGGTGAAATCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTgg < 1:132895/71‑1 (MQ=255) aGGCGGTGAAATCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTgg < 1:1577981/71‑1 (MQ=255) aaaTCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGcgcg < 1:3771425/70‑1 (MQ=255) aaaTCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGcgcg > 1:3719056/1‑70 (MQ=255) aaTCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGa < 1:1269240/71‑1 (MQ=255) aaTCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGa < 1:4232620/71‑1 (MQ=255) aaTCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGa < 1:2868212/71‑1 (MQ=255) aTCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGAt > 1:966918/1‑71 (MQ=255) aTTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCTGATTGa < 1:736815/71‑1 (MQ=255) aTTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGa < 1:2858142/71‑1 (MQ=255) aTTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGa < 1:3297150/71‑1 (MQ=255) aTTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGa > 1:3760066/1‑71 (MQ=255) accGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGAt < 1:2119028/70‑1 (MQ=255) cgTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAAcgggccg > 1:696390/1‑71 (MQ=255) tttCAACTGACCTTACATGGCGAGCGCTACCGGCTgg < 1:1231109/37‑1 (MQ=255) tttCAACTGACCTTACATGGCGAGCGCTACCGGCTgg < 1:2869286/37‑1 (MQ=255) tttCAACTGACCTTACATGGCGAGCGCTACCGGCTgg < 1:4384648/37‑1 (MQ=255) tttCAACTGACCTTACATGGCGAGCGCTACCGGCTgg < 1:3480770/37‑1 (MQ=255) tttCAACTGACCTTACATGGCGAGCGCTACCGGCTgg < 1:4061425/37‑1 (MQ=255) ttCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGaa < 1:2709391/56‑1 (MQ=255) ttCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGaa < 1:4448649/56‑1 (MQ=255) tCAACTGACCTTACATGGCGAGCGCTACCGGCTggcg > 1:771764/1‑37 (MQ=255) tCAACTGACCTTACATGGCGAGCGCTACCGGCTggcg > 1:5076116/1‑37 (MQ=255) tCAACTGACCTTACATGGCGAGCGCTACCGGCTggcg > 1:2444355/1‑37 (MQ=255) tCAACTGACCTTACATGGCGAGCGCTACCGGCTggcg > 1:3635679/1‑37 (MQ=255) aCTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCATTc < 1:1850161/71‑1 (MQ=255) cTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCATTcc < 1:2385234/71‑1 (MQ=255) cTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCATTcc < 1:4020489/71‑1 (MQ=255) aCCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCATTCCGc < 1:1383981/70‑1 (MQ=255) aCCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCATTCCGc < 1:523933/70‑1 (MQ=255) aCCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCATTCCGc < 1:2519822/70‑1 (MQ=255) ttACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGa < 1:3310841/38‑1 (MQ=255) ttACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGa < 1:913331/38‑1 (MQ=255) acacGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAg > 1:3072936/1‑44 (MQ=255) acacGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCa > 1:3211142/1‑59 (MQ=255) acacGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCa > 1:628117/1‑59 (MQ=255) acacGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCa > 1:854362/1‑59 (MQ=255) acacGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCa > 1:2613749/1‑59 (MQ=255) acacGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCATTCCGCCTCAtt > 1:3720226/1‑71 (MQ=255) | GGGGCCGGAAGGCGGTGAAATCATTCTGCGTACCCGCACCGCGTTTCAACTGACCTTACACGGCGAGCGCTACCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCGGGCATTCCGCCTCATT > Exported/2343032‑2343159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |