Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 2,359,876 | C→T | 27.6% | noncoding (1219/2905 nt) | rrlA ← | 23S ribosomal RNA |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 2,359,876 | 0 | C | T | 27.6% | 287.7 / 111.7 | 156 | noncoding (1219/2905 nt) | rrlA | 23S ribosomal RNA |
Reads supporting (aligned to +/- strand): ref base C (39/74); new base T (31/12); total (70/86) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.97e-05 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 |
Only 100 of 156 total aligned reads displayed.
TTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTG > Exported/2359821‑2359945 | ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt > 1:2972836/1‑71 (MQ=34) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa > 1:3754368/1‑68 (MQ=35) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACa < 1:172415/70‑1 (MQ=35) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:1532954/71‑1 (MQ=34) tACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGa > 1:355594/1‑70 (MQ=255) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcac < 1:3671486/58‑1 (MQ=33) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcac < 1:5185380/58‑1 (MQ=33) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcac < 1:4116475/58‑1 (MQ=33) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa < 1:3074979/70‑1 (MQ=35) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa < 1:2224595/70‑1 (MQ=35) tATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa < 1:4223250/62‑1 (MQ=35) tATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa < 1:3633800/62‑1 (MQ=35) tGTCAGGATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc > 1:669856/1‑71 (MQ=21) tGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc > 1:2942111/1‑71 (MQ=34) tGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc > 1:2695897/1‑71 (MQ=34) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCt < 1:3206644/70‑1 (MQ=255) cATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc > 1:967328/1‑71 (MQ=255) cTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca < 1:661449/71‑1 (MQ=35) cTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg > 1:2943447/1‑70 (MQ=255) cTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg < 1:2063386/71‑2 (MQ=255) cTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg < 1:8550/71‑2 (MQ=255) ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCtt < 1:400776/44‑1 (MQ=11) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca > 1:743714/1‑70 (MQ=40) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:277183/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:2875357/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:295439/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:1129106/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:3614209/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:3930489/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:4423917/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:5020869/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:549057/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:88289/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:117889/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:1632501/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:126803/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:2601325/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:1814784/1‑35 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa > 1:3180386/1‑58 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa > 1:2324336/1‑58 (MQ=11) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATa < 1:3050231/68‑1 (MQ=40) tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:1215444/38‑1 (MQ=40) tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:525227/38‑1 (MQ=40) tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:2448095/38‑1 (MQ=40) tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:3843980/38‑1 (MQ=40) tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:1649474/38‑1 (MQ=40) tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:3850218/38‑1 (MQ=40) tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGa < 1:4220735/39‑1 (MQ=40) tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGa < 1:3372018/39‑1 (MQ=40) tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGa < 1:2368094/39‑1 (MQ=40) tCCAGCATGACTCACAGCACACCTTCACAGGCTTACAGa < 1:3443849/39‑1 (MQ=255) ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa > 1:52195/1‑57 (MQ=35) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa > 1:4032354/1‑38 (MQ=40) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa > 1:2036929/1‑38 (MQ=40) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa > 1:3492344/1‑38 (MQ=40) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa > 1:4988248/1‑38 (MQ=40) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGa > 1:2959700/1‑37 (MQ=40) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGc < 1:2456418/69‑1 (MQ=255) gCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGcc > 1:3661244/1‑71 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt < 1:214763/64‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt < 1:4429718/64‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt < 1:3976395/64‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt < 1:4536685/64‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt < 1:2001148/64‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt < 1:3410515/64‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt < 1:4096692/64‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt < 1:3344076/64‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt < 1:3277032/64‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt < 1:4918407/64‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt < 1:2479820/64‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCa > 1:1667763/1‑70 (MQ=255) ccTCACAGCACACCTTCACAGGCTTACAGAACGCTcccc > 1:122959/1‑39 (MQ=40) ccTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGc > 1:4754254/1‑71 (MQ=255) ccTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGc > 1:5029986/1‑71 (MQ=255) ccTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGc > 1:1404075/1‑71 (MQ=255) cTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCt < 1:1010785/71‑1 (MQ=255) cTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCt < 1:2878941/71‑1 (MQ=255) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca < 1:4291835/49‑1 (MQ=40) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca < 1:2750521/49‑1 (MQ=40) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca < 1:2717933/49‑1 (MQ=40) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca < 1:1483256/49‑1 (MQ=40) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca < 1:3798890/49‑1 (MQ=40) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca < 1:1816783/49‑1 (MQ=40) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca < 1:4149547/49‑1 (MQ=40) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca < 1:4925513/49‑1 (MQ=40) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca < 1:4158218/49‑1 (MQ=40) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca < 1:4606992/49‑1 (MQ=40) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca < 1:4406559/49‑1 (MQ=40) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca < 1:3989978/51‑1 (MQ=255) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca < 1:4583073/51‑1 (MQ=255) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca < 1:4552950/51‑1 (MQ=255) cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac < 1:4670076/44‑1 (MQ=38) cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac < 1:2231432/44‑1 (MQ=38) cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac < 1:2654295/44‑1 (MQ=38) cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac < 1:756965/44‑1 (MQ=38) cacaGCACACCTTCAAAGGCTTACAGAACGCTCCCCTACCcaac < 1:4263152/44‑1 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca > 1:2119151/1‑49 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCg > 1:4796816/1‑71 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCg > 1:3753824/1‑71 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCg > 1:2598557/1‑71 (MQ=255) | TTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTG > Exported/2359821‑2359945 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |