Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,359,876 C→T 27.6% noncoding (1219/2905 nt) rrlA ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,359,8760CT27.6% 287.7 / 111.7 156noncoding (1219/2905 nt)rrlA23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (39/74);  new base T (31/12);  total (70/86)
Fisher's exact test for biased strand distribution p-value = 2.97e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 156 total aligned reads displayed.

TTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTG  >  Exported/2359821‑2359945
                                                       |                                                                     
ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                                        >  1:2972836/1‑71 (MQ=34)
     gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                                      >  1:3754368/1‑68 (MQ=35)
     gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACa                                                    <  1:172415/70‑1 (MQ=35)
     gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                                   <  1:1532954/71‑1 (MQ=34)
       tACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGa                                                  >  1:355594/1‑70 (MQ=255)
        aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcac                                                             <  1:3671486/58‑1 (MQ=33)
        aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcac                                                             <  1:5185380/58‑1 (MQ=33)
        aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcac                                                             <  1:4116475/58‑1 (MQ=33)
        aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa                                                 <  1:3074979/70‑1 (MQ=35)
        aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa                                                 <  1:2224595/70‑1 (MQ=35)
           tATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                                      <  1:4223250/62‑1 (MQ=35)
           tATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                                      <  1:3633800/62‑1 (MQ=35)
             tGTCAGGATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                                           >  1:669856/1‑71 (MQ=21)
             tGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                                           >  1:2942111/1‑71 (MQ=34)
             tGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcc                                           >  1:2695897/1‑71 (MQ=34)
                 aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCt                                        <  1:3206644/70‑1 (MQ=255)
                   cATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAcc                                     >  1:967328/1‑71 (MQ=255)
                           cTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                             <  1:661449/71‑1 (MQ=35)
                           cTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg                             >  1:2943447/1‑70 (MQ=255)
                           cTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg                             <  1:2063386/71‑2 (MQ=255)
                           cTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg                             <  1:8550/71‑2 (MQ=255)
                            ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCtt                                                       <  1:400776/44‑1 (MQ=11)
                              cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                           >  1:743714/1‑70 (MQ=40)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:277183/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:2875357/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:295439/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:1129106/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:3614209/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:3930489/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:4423917/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:5020869/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:549057/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:88289/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:117889/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:1632501/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:126803/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:2601325/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTca                                                              >  1:1814784/1‑35 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa                                       >  1:3180386/1‑58 (MQ=11)
                              cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa                                       >  1:2324336/1‑58 (MQ=11)
                                  tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATa                         <  1:3050231/68‑1 (MQ=40)
                                      tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                                   <  1:1215444/38‑1 (MQ=40)
                                      tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                                   <  1:525227/38‑1 (MQ=40)
                                      tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                                   <  1:2448095/38‑1 (MQ=40)
                                      tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                                   <  1:3843980/38‑1 (MQ=40)
                                      tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                                   <  1:1649474/38‑1 (MQ=40)
                                      tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                                   <  1:3850218/38‑1 (MQ=40)
                                      tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGa                                                  <  1:4220735/39‑1 (MQ=40)
                                      tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGa                                                  <  1:3372018/39‑1 (MQ=40)
                                      tCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGa                                                  <  1:2368094/39‑1 (MQ=40)
                                      tCCAGCATGACTCACAGCACACCTTCACAGGCTTACAGa                                                  <  1:3443849/39‑1 (MQ=255)
                                       ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                               >  1:52195/1‑57 (MQ=35)
                                        cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa                                                 >  1:4032354/1‑38 (MQ=40)
                                        cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa                                                 >  1:2036929/1‑38 (MQ=40)
                                        cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa                                                 >  1:3492344/1‑38 (MQ=40)
                                        cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa                                                 >  1:4988248/1‑38 (MQ=40)
                                        cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGa                                                  >  1:2959700/1‑37 (MQ=40)
                                        cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGc                  <  1:2456418/69‑1 (MQ=255)
                                          gCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGcc              >  1:3661244/1‑71 (MQ=255)
                                              gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt                 <  1:214763/64‑1 (MQ=255)
                                              gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt                 <  1:4429718/64‑1 (MQ=255)
                                              gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt                 <  1:3976395/64‑1 (MQ=255)
                                              gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt                 <  1:4536685/64‑1 (MQ=255)
                                              gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt                 <  1:2001148/64‑1 (MQ=255)
                                              gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt                 <  1:3410515/64‑1 (MQ=255)
                                              gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt                 <  1:4096692/64‑1 (MQ=255)
                                              gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt                 <  1:3344076/64‑1 (MQ=255)
                                              gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt                 <  1:3277032/64‑1 (MQ=255)
                                              gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt                 <  1:4918407/64‑1 (MQ=255)
                                              gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt                 <  1:2479820/64‑1 (MQ=255)
                                              gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCa           >  1:1667763/1‑70 (MQ=255)
                                               ccTCACAGCACACCTTCACAGGCTTACAGAACGCTcccc                                         >  1:122959/1‑39 (MQ=40)
                                               ccTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGc         >  1:4754254/1‑71 (MQ=255)
                                               ccTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGc         >  1:5029986/1‑71 (MQ=255)
                                               ccTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGc         >  1:1404075/1‑71 (MQ=255)
                                                cTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCt        <  1:1010785/71‑1 (MQ=255)
                                                cTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCt        <  1:2878941/71‑1 (MQ=255)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                             <  1:4291835/49‑1 (MQ=40)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                             <  1:2750521/49‑1 (MQ=40)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                             <  1:2717933/49‑1 (MQ=40)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                             <  1:1483256/49‑1 (MQ=40)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                             <  1:3798890/49‑1 (MQ=40)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                             <  1:1816783/49‑1 (MQ=40)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                             <  1:4149547/49‑1 (MQ=40)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                             <  1:4925513/49‑1 (MQ=40)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                             <  1:4158218/49‑1 (MQ=40)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                             <  1:4606992/49‑1 (MQ=40)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca                             <  1:4406559/49‑1 (MQ=40)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                           <  1:3989978/51‑1 (MQ=255)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                           <  1:4583073/51‑1 (MQ=255)
                                                 tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                           <  1:4552950/51‑1 (MQ=255)
                                                  cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac                                 <  1:4670076/44‑1 (MQ=38)
                                                  cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac                                 <  1:2231432/44‑1 (MQ=38)
                                                  cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac                                 <  1:2654295/44‑1 (MQ=38)
                                                  cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac                                 <  1:756965/44‑1 (MQ=38)
                                                  cacaGCACACCTTCAAAGGCTTACAGAACGCTCCCCTACCcaac                                 <  1:4263152/44‑1 (MQ=255)
                                                   acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca                           >  1:2119151/1‑49 (MQ=255)
                                                   acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCg     >  1:4796816/1‑71 (MQ=255)
                                                   acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCg     >  1:3753824/1‑71 (MQ=255)
                                                   acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCg     >  1:2598557/1‑71 (MQ=255)
                                                       |                                                                     
TTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTG  >  Exported/2359821‑2359945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: