Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,396,417 T→C 9.5% K257K (AAA→AAG corA ← magnesium/nickel/cobalt transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,396,4170TC 9.5% 238.7 / 12.8 84K257K (AAA→AAGcorAmagnesium/nickel/cobalt transporter
Reads supporting (aligned to +/- strand):  ref base T (30/46);  new base C (3/5);  total (33/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

AGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTGG  >  Exported/2396350‑2396486
                                                                   |                                                                     
aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                                     >  1:5139216/1‑70 (MQ=255)
aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                                     >  1:3837953/1‑70 (MQ=255)
aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                                     >  1:3351686/1‑70 (MQ=255)
aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                                     >  1:3221235/1‑70 (MQ=255)
aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                                     >  1:2932107/1‑70 (MQ=255)
aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                                     >  1:2085929/1‑70 (MQ=255)
  ttCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat                                                                  <  1:401522/71‑1 (MQ=255)
  ttCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat                                                                  <  1:1142886/71‑1 (MQ=255)
      tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACTGAGAAGATTTTgatgat                                                               >  1:3294223/1‑70 (MQ=255)
      tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTgatgat                                                               >  1:98226/1‑70 (MQ=255)
      tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGatgatg                                                              >  1:2458664/1‑71 (MQ=255)
             gCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCt                                                       >  1:2531082/1‑71 (MQ=255)
             gCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCt                                                       >  1:3331190/1‑71 (MQ=255)
                 gAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTc                                                   <  1:2526692/71‑1 (MQ=255)
                 gAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGAAGATGCGGTTCTGCTc                                                   <  1:4773092/71‑1 (MQ=255)
                  aaGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat                                                                  <  1:1574528/55‑1 (MQ=255)
                   aGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGa                                                 <  1:2167132/71‑1 (MQ=255)
                   aGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGCTCTGCTCGa                                                 <  1:3130823/71‑1 (MQ=255)
                    gCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGAt                                                <  1:3354515/71‑1 (MQ=255)
                             gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                                       <  1:549962/71‑1 (MQ=255)
                             gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                                       <  1:2447158/71‑1 (MQ=255)
                             gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                                       <  1:1813713/71‑1 (MQ=255)
                             gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                                       <  1:5057904/71‑1 (MQ=255)
                             gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                                       <  1:5072763/71‑1 (MQ=255)
                              tCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATaaaa                                      >  1:258502/1‑71 (MQ=255)
                                ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaagc                                     >  1:4217374/1‑68 (MQ=255)
                                ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATGAAGcc                                    >  1:1692191/1‑71 (MQ=255)
                                ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATGAAGcc                                    >  1:4184570/1‑71 (MQ=255)
                                    gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                                          <  1:3384193/61‑1 (MQ=255)
                                    gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                                          <  1:3557998/61‑1 (MQ=255)
                                    gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                                          <  1:4141456/61‑1 (MQ=255)
                                    gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                                          <  1:1228375/61‑1 (MQ=255)
                                    gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                                          <  1:1047516/61‑1 (MQ=255)
                                    gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                                          <  1:1777772/61‑1 (MQ=255)
                                    gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAtt                                <  1:2477986/71‑1 (MQ=255)
                                    gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAtt                                <  1:1087850/71‑1 (MQ=255)
                                     cAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt                                 >  1:3800745/1‑69 (MQ=255)
                                     cAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt                                 >  1:812618/1‑69 (MQ=255)
                                      aGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATaa                                        >  1:251984/1‑61 (MQ=255)
                                        gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt                                 >  1:2162886/1‑66 (MQ=255)
                                        gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt                                 >  1:1675692/1‑66 (MQ=255)
                                        gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt                                 >  1:1695809/1‑66 (MQ=255)
                                          ataccacggataccacggAGAAGATTTTGATGCTGCGGTTCTGCTCGATGTTGATAAAAccc                                   >  1:2891766/1‑62 (MQ=255)
                                          aTACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATa                                         >  1:4469958/1‑56 (MQ=255)
                                          aTACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAAccc                                   >  1:4330856/1‑62 (MQ=255)
                                           tACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCt                         >  1:1174252/1‑71 (MQ=255)
                                            aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc                                                     <  1:3525462/42‑1 (MQ=255)
                                            aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc                                                     <  1:699964/42‑1 (MQ=255)
                                            aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc                                                     <  1:1383812/42‑1 (MQ=255)
                                            aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc                                                     <  1:1085906/42‑1 (MQ=255)
                                             ccACGGATACCACCGAGAAGATTTTTATGATGCTGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc                       <  1:2257654/71‑1 (MQ=255)
                                             ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCTATGTTGATAAAACCCATTGCCGCCTGc                       <  1:4529187/71‑1 (MQ=255)
                                             ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc                       <  1:4311684/71‑1 (MQ=255)
                                             ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc                       <  1:824486/71‑1 (MQ=255)
                                              cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg                                               <  1:1138150/46‑1 (MQ=255)
                                              cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg                                               <  1:2826970/46‑1 (MQ=255)
                                              cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg                                               <  1:1131899/46‑1 (MQ=255)
                                               aCGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTc                                                   <  1:2991302/41‑1 (MQ=255)
                                               aCGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCAt                     >  1:4366902/1‑71 (MQ=255)
                                                 ggATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCAt                     >  1:2249070/1‑69 (MQ=255)
                                                 ggATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCAt                     >  1:3429463/1‑69 (MQ=255)
                                                   aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATaaaa                                      >  1:773147/1‑50 (MQ=255)
                                                   aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt                                 >  1:3814045/1‑55 (MQ=255)
                                                   aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt                                 >  1:2268030/1‑55 (MQ=255)
                                                   aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt                                 >  1:1645515/1‑55 (MQ=255)
                                                   aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt                                 >  1:1741983/1‑55 (MQ=255)
                                                   aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt                                 >  1:4984013/1‑55 (MQ=255)
                                                   aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt                                 >  1:3312330/1‑55 (MQ=255)
                                                   aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAgg                 <  1:2766635/71‑1 (MQ=255)
                                                   aTACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAgg                 <  1:642244/71‑1 (MQ=255)
                                                   aTACCACCGAGAAGATTTAGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAgg                 <  1:698915/71‑1 (MQ=255)
                                                    tACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGa                <  1:4877332/71‑1 (MQ=255)
                                                    tACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGa                <  1:4751950/71‑1 (MQ=255)
                                                      ccaccGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAg              <  1:3926750/71‑1 (MQ=255)
                                                      ccaccGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAg              <  1:1530667/71‑1 (MQ=255)
                                                      ccaccGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAACCCATTGCCGCCTGCATCAGGAAg              <  1:3034435/70‑1 (MQ=255)
                                                           gagaAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGa                <  1:1948/64‑1 (MQ=255)
                                                             gaagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAg              >  1:3563058/1‑64 (MQ=255)
                                                             gaagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAg              >  1:5023836/1‑64 (MQ=255)
                                                             gaagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCa          <  1:822/68‑1 (MQ=255)
                                                             gaagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCa          <  1:1360881/68‑1 (MQ=255)
                                                              aagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCtt      <  1:577200/71‑1 (MQ=255)
                                                              aagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCtt      <  1:1391931/71‑1 (MQ=255)
                                                              aagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCtt      <  1:742254/71‑1 (MQ=255)
                                                              aagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCtt      <  1:3319705/71‑1 (MQ=255)
                                                              aagaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCtt      <  1:1644396/71‑1 (MQ=255)
                                                               agaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCtt      <  1:4397177/70‑1 (MQ=255)
                                                                gaTTTTGATGATGCGGTTCTGCTCGATGTTGATaaaa                                      >  1:2043189/1‑37 (MQ=255)
                                                                gaTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGAATCAGGAAGTTCACCTTCt    >  1:4182288/1‑71 (MQ=255)
                                                                 aTTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTg   <  1:3919714/71‑1 (MQ=255)
                                                                  ttttGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTgg  <  1:1596639/71‑1 (MQ=255)
                                                                  ttttGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTgg  <  1:1821522/71‑1 (MQ=255)
                                                                  ttttGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTgg  <  1:2935005/71‑1 (MQ=255)
                                                                  ttttGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTgg  <  1:169153/71‑1 (MQ=255)
                                                                  ttttGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTg   <  1:2144787/70‑1 (MQ=255)
                                                                  ttttGATGATGCGGTGCTGCGCGATGGTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTgg  <  1:3284914/71‑1 (MQ=255)
                                                                   tttGATGATGCGGTTCTGCTCGATGTTGATAAAAccc                                   >  1:654501/1‑37 (MQ=255)
                                                                   |                                                                     
AGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCATCAGGAAGTTCACCTTCTGG  >  Exported/2396350‑2396486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: