Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,539,542 A→G 5.0% N23D (AAT→GAT)  rfaJ → UDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,539,5420AG 5.0% 540.4 / 12.7 178N23D (AAT→GAT) rfaJUDP‑D‑glucose:(galactosyl)lipopolysaccharide glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (105/64);  new base G (3/6);  total (108/70)
Fisher's exact test for biased strand distribution p-value = 1.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 190 total aligned reads displayed.

CATTGTGGACTCATTTCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGGTGTAGGTGTTTCCAT  >  Exported/2539472‑2539612
                                                                      |                                                                      
cATTGTGGACTCATTTCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATaa                                                                        <  1:271841/71‑1 (MQ=255)
    gtgGACTCATTTCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATaaa                                                                       >  1:894027/1‑68 (MQ=255)
    gtgGACTCATTTCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATaaa                                                                       >  1:479711/1‑68 (MQ=255)
    gtgGACTCATTTCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATAc                                                                    >  1:1544091/1‑71 (MQ=255)
     tgGACTCATTTCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACt                                                                   <  1:5053456/71‑1 (MQ=255)
     tgGACTCATTTCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACt                                                                   <  1:1556911/71‑1 (MQ=255)
       gACTCATTTCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTc                                                                 >  1:1041968/1‑71 (MQ=255)
       gACTCATTTCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTc                                                                 >  1:5093193/1‑71 (MQ=255)
       gACTCATTTCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTc                                                                 >  1:3562133/1‑71 (MQ=255)
            aTTTCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGaa                                                             >  1:3354258/1‑70 (MQ=255)
            aTTTCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAAt                                                            >  1:4716965/1‑71 (MQ=255)
             tttCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAAt                                                                      <  1:951515/60‑1 (MQ=255)
             tttCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAAt                                                                      <  1:719592/60‑1 (MQ=255)
               tCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGtt                                                         >  1:1159016/1‑71 (MQ=255)
                    ccATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATaaa                                                                       >  1:4554615/1‑52 (MQ=255)
                         gagaTAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGc                                               >  1:3186317/1‑71 (MQ=255)
                            atagataAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTc                                                                 >  1:1735659/1‑50 (MQ=255)
                            atagataAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGttt                                                        <  1:2997468/59‑1 (MQ=255)
                            atagataAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGttt                                                        <  1:3992081/59‑1 (MQ=255)
                              agataAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAAt                                                                      >  1:2030178/1‑43 (MQ=255)
                              agataAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTaa                                                      >  1:102041/1‑59 (MQ=255)
                                ataAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTAt                                           <  1:5124941/68‑1 (MQ=255)
                                  aaaGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATg                                                   >  1:341004/1‑58 (MQ=255)
                                  aaaGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATg                                                   >  1:1820096/1‑58 (MQ=255)
                                  aaaGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATg                                                   >  1:1979686/1‑58 (MQ=255)
                                  aaaGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATg                                                   >  1:2394370/1‑58 (MQ=255)
                                  aaaGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATg                                                   >  1:3495212/1‑58 (MQ=255)
                                  aaaGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATg                                                   >  1:2082868/1‑58 (MQ=255)
                                  aaaGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATg                                                   >  1:698487/1‑58 (MQ=255)
                                  aaaGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGgtgt                                      >  1:1176411/1‑71 (MQ=255)
                                  aaaGTTAAAGCATGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATg                                                   >  1:587970/1‑58 (MQ=255)
                                   aaGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTc                                     >  1:3651595/1‑71 (MQ=255)
                                     cttAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCg                                    <  1:1434364/69‑1 (MQ=255)
                                      ttAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTc                                     <  1:2204843/68‑1 (MQ=255)
                                      ttAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTc                                     <  1:3973043/68‑1 (MQ=255)
                                         aaGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTa                                            >  1:460064/1‑58 (MQ=255)
                                         aaGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTa                                            >  1:4080080/1‑58 (MQ=255)
                                         aaGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTa                                            >  1:4127425/1‑58 (MQ=255)
                                         aaGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCt                               <  1:1587479/71‑1 (MQ=255)
                                         aaGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCt                               <  1:3480520/71‑1 (MQ=255)
                                         aaGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCt                               <  1:138713/71‑1 (MQ=255)
                                          aGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATgg                                         >  1:2614107/1‑60 (MQ=255)
                                          aGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATgg                                         >  1:1084885/1‑60 (MQ=255)
                                          aGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATgg                                         >  1:1618106/1‑60 (MQ=255)
                                          aGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCt                               >  1:3024428/1‑70 (MQ=255)
                                             cTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAAtt                           <  1:4226841/71‑1 (MQ=255)
                                              tGGGAGTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGc                                >  1:1297588/1‑65 (MQ=255)
                                               gggATTTTCGGCTAGCTAATATAGATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCt                               <  1:1809886/65‑1 (MQ=255)
                                               gggATTTTCGGCTAGCTAATATAGATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCt                               <  1:1909863/65‑1 (MQ=255)
                                               gggATTTTCGGCTAGCTAATATAGATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCt                               <  1:2267171/65‑1 (MQ=255)
                                               gggATTTTCGGCTAGCTAATATAGATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCt                               <  1:3199887/65‑1 (MQ=255)
                                                  aTTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCtt                      >  1:2141948/1‑71 (MQ=255)
                                                   ttttCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATg                                                   >  1:4696368/1‑41 (MQ=255)
                                                   ttttCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGt                                                  >  1:4836896/1‑42 (MQ=255)
                                                    tttCGGCTAGCTAATATAAATACTTCTGAATGTTTaaa                                                     >  1:2968016/1‑38 (MQ=255)
                                                    tttCGGCTAGCTAATATAAATACTTCTGAATGTTTaaa                                                     >  1:433811/1‑38 (MQ=255)
                                                    tttCGGCTAGCTAATATAAATACTTCTGAATGTTTaaa                                                     >  1:2545416/1‑38 (MQ=255)
                                                    tttCGGCTAGCTAATATAAATACTTCTGAATGTTTaaa                                                     >  1:618028/1‑38 (MQ=255)
                                                    tttCGGCTAGCTAATATAAATACTTCTGAATGTTTaaa                                                     >  1:4625293/1‑38 (MQ=255)
                                                     ttCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCg                                    >  1:1568003/1‑54 (MQ=255)
                                                     ttCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGc                                >  1:839120/1‑58 (MQ=255)
                                                     ttCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGAt                   >  1:2046809/1‑71 (MQ=255)
                                                      tCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTg                                                >  1:2445290/1‑41 (MQ=255)
                                                      tCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATg                  >  1:3254331/1‑71 (MQ=255)
                                                       cGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCt                                             <  1:4159294/43‑1 (MQ=255)
                                                       cGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCt                                             <  1:1206250/43‑1 (MQ=255)
                                                       cGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCt                                             <  1:4815415/43‑1 (MQ=255)
                                                       cGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCt                                             <  1:4911677/43‑1 (MQ=255)
                                                       cGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCt                                             <  1:1684257/43‑1 (MQ=255)
                                                         gctagctaATATAAATACTTCTGAATGTTTAAATGTTGCCTa                                            <  1:3319440/42‑1 (MQ=255)
                                                         gctagctaATATAAATACTTCTGAATGTTTAAATGTTGCCTa                                            <  1:2860599/42‑1 (MQ=255)
                                                         gctagctaATATAAATACTTCTGAATGTTTAAATGTTGCCTa                                            <  1:2825533/42‑1 (MQ=255)
                                                         gctagctaATATAAATACTTCTGAATGTTTAAATGTTGCCTa                                            <  1:2751519/42‑1 (MQ=255)
                                                         gctagctaATATAAATACTTCTGAATGTTTAAATGTTGCCTa                                            <  1:2223867/42‑1 (MQ=255)
                                                         gctagctaATATAAATACTTCTGAATGTTTAAATGTTGCCTa                                            <  1:1097984/42‑1 (MQ=255)
                                                          ctagctaATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGgtg               >  1:4943923/1‑70 (MQ=255)
                                                           tagctaATATAAATACTTCTGAATGTTTAAATGTTGCCTATg                                          >  1:1166131/1‑42 (MQ=255)
                                                           tagctaATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGgtg               >  1:4385634/1‑69 (MQ=255)
                                                           tagctaATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGATAATTATCt                       >  1:1700398/1‑61 (MQ=255)
                                                             gctaATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAAtt                           >  1:550361/1‑55 (MQ=255)
                                                             gctaATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAAtt                           >  1:3349544/1‑55 (MQ=255)
                                                             gctaATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAAtt                           >  1:3442592/1‑55 (MQ=255)
                                                             gctaATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAAtt                           >  1:2158457/1‑55 (MQ=255)
                                                             gctaATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATTGTGTa             >  1:3827535/1‑69 (MQ=255)
                                                             gctaATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGGTGTAgg           >  1:1258882/1‑71 (MQ=255)
                                                              ctaATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGgtgt              <  1:2538156/67‑1 (MQ=255)
                                                                aaTATAAATACTTCTGAATGTTTAAATGTTGCCTATGgt                                        <  1:770033/39‑1 (MQ=255)
                                                                 atatAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGAt                                  <  1:5074635/44‑1 (MQ=255)
                                                                 atatAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGGTGTAGGTGtt       >  1:441279/1‑71 (MQ=255)
                                                                  tataAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGGTGTAGgt          <  1:1582754/67‑1 (MQ=255)
                                                                  tataAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGGTGTAGgt          <  1:4975940/67‑1 (MQ=255)
                                                                  tataAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGGTGTAGgt          <  1:2702266/67‑1 (MQ=255)
                                                                    taAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCg                                    <  1:1448825/39‑1 (MQ=255)
                                                                    taAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCg                                    <  1:1626451/39‑1 (MQ=255)
                                                                    taAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCg                                    <  1:2338985/39‑1 (MQ=255)
                                                                    taAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCg                                    <  1:3781795/39‑1 (MQ=255)
                                                                      aaTACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGGTGTAGGTGTTTCcat  <  1:2091411/71‑1 (MQ=255)
                                                                      aaTACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGGTGTAGGTGTTTCcat  >  1:3281232/1‑71 (MQ=255)
                                                                      aaTACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGGTGTAGGTGTTTCcat  <  1:1514310/71‑1 (MQ=255)
                                                                      aaTACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGGTGTAGGTGTTTCcat  <  1:3685833/71‑1 (MQ=255)
                                                                      |                                                                      
CATTGTGGACTCATTTCCTGCCATAGAGATAGATAAAGTTAAAGCCTGGGATTTTCGGCTAGCTAATATAAATACTTCTGAATGTTTAAATGTTGCCTATGGTGTCGATGCTAATTATCTTGATGGTGTAGGTGTTTCCAT  >  Exported/2539472‑2539612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: