Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,595,746 G→A 7.5% I284I (ATC→ATT arsB ← arsenite/antimonite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,595,7460GA 7.5% 368.2 / 14.0 120I284I (ATC→ATTarsBarsenite/antimonite transporter
Reads supporting (aligned to +/- strand):  ref base G (56/55);  new base A (6/3);  total (62/58)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 133 total aligned reads displayed.

CCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGC  >  Exported/2595678‑2595811
                                                                    |                                                                 
ccAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATGAACCACCAGATACATGCCGAGCGAGAAGAt                                                                 >  1:1517447/1‑71 (MQ=255)
     aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                                            <  1:423141/71‑1 (MQ=255)
     aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                                            <  1:4456247/71‑1 (MQ=255)
     aaGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACaa                                                            <  1:4489240/71‑1 (MQ=255)
      aGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                           <  1:1029546/71‑1 (MQ=255)
           ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                      <  1:1283160/71‑1 (MQ=255)
           ttCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                      <  1:470645/71‑1 (MQ=255)
            tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGa                                                               >  1:318921/1‑61 (MQ=255)
            tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                              >  1:279900/1‑62 (MQ=255)
            tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                              >  1:814021/1‑62 (MQ=255)
            tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                              >  1:2813726/1‑62 (MQ=255)
            tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                              >  1:219767/1‑62 (MQ=255)
            tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                              >  1:3699924/1‑62 (MQ=255)
            tCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATATGc                                                       >  1:2082750/1‑69 (MQ=255)
                ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                             <  1:2745119/59‑1 (MQ=255)
                ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                             <  1:4329728/59‑1 (MQ=255)
                ttAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                             <  1:657498/59‑1 (MQ=255)
                      cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                     >  1:3516885/1‑61 (MQ=255)
                      cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                     >  1:110463/1‑61 (MQ=255)
                      cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                     >  1:2252767/1‑61 (MQ=255)
                      cGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGAGAGAAGATGACAATCTGCCa                                                     >  1:70461/1‑61 (MQ=255)
                       ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc                                          <  1:2963143/71‑1 (MQ=255)
                       ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc                                          <  1:736425/71‑1 (MQ=255)
                       ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc                                          <  1:2506876/71‑1 (MQ=255)
                       ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc                                          <  1:4508988/71‑1 (MQ=255)
                       ggCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACcgc                                          <  1:4911483/71‑1 (MQ=255)
                        gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                           >  1:1684842/1‑53 (MQ=255)
                        gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                           >  1:2824700/1‑53 (MQ=255)
                        gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                           >  1:3807976/1‑53 (MQ=255)
                        gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                           >  1:4869153/1‑53 (MQ=255)
                        gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                           >  1:3783625/1‑53 (MQ=255)
                        gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                           >  1:165568/1‑53 (MQ=255)
                        gCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAAt                                                           >  1:3557356/1‑53 (MQ=255)
                             gcgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGa                                    >  1:4760159/1‑71 (MQ=255)
                              cgcAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGAc                                   >  1:3986230/1‑71 (MQ=255)
                                    ccATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                              >  1:3544914/1‑38 (MQ=255)
                                    ccATAAACCACCAGATACATGCCGAGCGAGAAGATGAc                                                              >  1:1058160/1‑38 (MQ=255)
                                     cATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                             <  1:2543655/38‑1 (MQ=255)
                                     cATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                             <  1:2160791/38‑1 (MQ=255)
                                     cATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                             <  1:4625424/38‑1 (MQ=255)
                                     cATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                             <  1:1837648/38‑1 (MQ=255)
                                     cATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                             <  1:1283598/38‑1 (MQ=255)
                                     cATAAACCACCAGATACATGCCGAGCGAGAAGATGACa                                                             <  1:1058710/38‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                      <  1:2710958/43‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                      <  1:2655549/43‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                      <  1:2643766/43‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGcc                                                      <  1:4440418/43‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                       <  1:474156/42‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                       <  1:4839466/42‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                       <  1:944278/42‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                       <  1:3356256/42‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                       <  1:3072604/42‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                       <  1:2562069/42‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                       <  1:2890437/42‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCTAGGGGGCACCGCGCAGGACTTTACCCg                           >  1:1994388/1‑70 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc                                             <  1:5053945/52‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc                                             <  1:1640059/52‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc                                             <  1:1278359/52‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc                                             <  1:1114187/52‑1 (MQ=255)
                                       tAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAc                                             <  1:3025739/52‑1 (MQ=255)
                                         aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGc                                                       <  1:3766233/40‑1 (MQ=255)
                                         aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCa                                                     >  1:1368904/1‑42 (MQ=255)
                                         aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                                   >  1:3203780/1‑44 (MQ=255)
                                         aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                                   >  1:728254/1‑44 (MQ=255)
                                         aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                                   >  1:1855670/1‑44 (MQ=255)
                                         aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                                   >  1:1564081/1‑44 (MQ=255)
                                         aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAgg                                                   >  1:4245624/1‑44 (MQ=255)
                                         aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTa                         <  1:489454/70‑1 (MQ=255)
                                         aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAt                        >  1:824228/1‑71 (MQ=255)
                                         aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAt                        >  1:3996750/1‑71 (MQ=255)
                                         aaCCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTAt                        >  1:1811029/1‑71 (MQ=255)
                                                aGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                  >  1:471589/1‑70 (MQ=255)
                                                 gATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCa                <  1:5047097/71‑1 (MQ=255)
                                                   tACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATg              <  1:4321383/71‑1 (MQ=255)
                                                   tACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATg              <  1:3476054/71‑1 (MQ=255)
                                                   tACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATg              <  1:3809251/71‑1 (MQ=255)
                                                   tACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGAAt               <  1:196274/70‑1 (MQ=255)
                                                    aCATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa             >  1:1059677/1‑71 (MQ=255)
                                                    aCATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGa             >  1:2745877/1‑71 (MQ=255)
                                                      aTGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                            >  1:4496596/1‑38 (MQ=255)
                                                      aTGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                            >  1:2969399/1‑38 (MQ=255)
                                                      aTGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                            >  1:2365532/1‑38 (MQ=255)
                                                      aTGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                            >  1:565496/1‑38 (MQ=255)
                                                      aTGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                            >  1:2449413/1‑38 (MQ=255)
                                                      aTGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCAcc                                            >  1:4860973/1‑38 (MQ=255)
                                                       tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTccg                            >  1:4369955/1‑52 (MQ=255)
                                                       tGCCCAGTGAGAAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGAcct          >  1:3760988/1‑70 (MQ=255)
                                                             gtgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCttttc    <  1:4383066/69‑2 (MQ=255)
                                                             gtgagaAAATAACGATCTGCCATGGCGCACCGCGCAGGACTTTTCCGGTGTTGATCGAATGACCTCttttc    <  1:3026550/69‑2 (MQ=255)
                                                               gagaAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCg                  <  1:1917654/55‑1 (MQ=255)
                                                               gagaAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGc  <  1:1119414/71‑1 (MQ=255)
                                                                agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                      <  1:786577/50‑1 (MQ=255)
                                                                agaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTa                      <  1:2224188/50‑1 (MQ=255)
                                                                 gaagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCa                >  1:2173623/1‑55 (MQ=255)
                                                                  aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg        <  1:3255475/62‑1 (MQ=255)
                                                                  aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg        <  1:3610010/62‑1 (MQ=255)
                                                                  aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg        <  1:2933304/62‑1 (MQ=255)
                                                                  aagaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACcgcg        <  1:414333/62‑1 (MQ=255)
                                                                   agaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTaa                     >  1:2548385/1‑48 (MQ=255)
                                                                    gaTGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGtt      >  1:4425836/1‑62 (MQ=255)
                                                                    |                                                                 
CCAGAAAGATATTCCGTTAATCCGGCATTGCGCAGGCCATAAACCACCAGATACATGCCGAGCGAGAAGATGACAATCTGCCAGGGGGCACCGCGCAGGACTTTACCCGTATTAATCGCATGACCGCGTTTAGC  >  Exported/2595678‑2595811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: