Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,935,934 A→G 6.4% intergenic (‑144/‑73) idnD ← / → idnK L‑idonate 5‑dehydrogenase, NAD‑binding/D‑gluconate kinase, thermosensitive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,935,9340AG 6.4% 379.3 / 11.0 125intergenic (‑144/‑73)idnD/idnKL‑idonate 5‑dehydrogenase, NAD‑binding/D‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand):  ref base A (42/75);  new base G (5/3);  total (47/78)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

GTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGG  >  Exported/2935868‑2935997
                                                                  |                                                               
gTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAACTaa                                                             <  1:2558896/71‑1 (MQ=255)
gTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaa                                                             <  1:3575580/71‑1 (MQ=255)
gTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCATATCAGTAGAAATaa                                                             <  1:1247257/71‑1 (MQ=255)
    gAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaa                                                         <  1:2907910/71‑1 (MQ=255)
    gAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaa                                                         <  1:2048694/71‑1 (MQ=255)
    gAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaa                                                         <  1:1998987/71‑1 (MQ=255)
    gAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaa                                                         <  1:4151531/71‑1 (MQ=255)
     aaaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaaa                                                        >  1:2424979/1‑71 (MQ=255)
     aaaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaaa                                                        >  1:1154622/1‑71 (MQ=255)
     aaaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaaa                                                        >  1:1232203/1‑71 (MQ=255)
     aaaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaaaa                                                        >  1:467614/1‑71 (MQ=255)
      aaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAAc                                                       >  1:1013219/1‑71 (MQ=255)
      aaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAAc                                                       >  1:2755272/1‑71 (MQ=255)
      aaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAAc                                                       >  1:4321911/1‑71 (MQ=255)
      aaaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAAc                                                       >  1:5120419/1‑71 (MQ=255)
       aaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACg                                                      >  1:1521886/1‑71 (MQ=255)
       aaTTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACg                                                      <  1:2166439/71‑1 (MQ=255)
           gTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGa                                                   >  1:3140759/1‑70 (MQ=255)
           gTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGa                                                   >  1:2454559/1‑70 (MQ=255)
           gTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGa                                                   >  1:1385122/1‑70 (MQ=255)
           gTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGa                                                   <  1:1212506/70‑1 (MQ=255)
           gTTACAGTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGa                                                   >  1:671443/1‑70 (MQ=255)
            ttACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAGATAAAAAAACGTGaaa                                                 >  1:3381903/1‑71 (MQ=255)
            ttACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAGATAAAAAAACGTGaaa                                                 >  1:2775186/1‑71 (MQ=255)
            ttACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAGATAAAAAAACGTGaaa                                                 >  1:2826770/1‑71 (MQ=255)
                aTTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat                                             <  1:4458163/71‑1 (MQ=255)
                 ttACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattatt                                            <  1:147104/71‑1 (MQ=255)
                    cTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATtattat                                          <  1:2746770/70‑1 (MQ=255)
                    cTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATtattat                                          <  1:2918105/70‑1 (MQ=255)
                    cTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATtattat                                          <  1:3591724/70‑1 (MQ=255)
                    cTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATtattat                                          <  1:2170862/70‑1 (MQ=255)
                    cTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATg                                         <  1:1050659/71‑1 (MQ=255)
                       tGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATgccg                                      >  1:3761154/1‑71 (MQ=255)
                        gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatta                                              <  1:3372482/62‑1 (MQ=255)
                        gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatta                                              <  1:1517914/62‑1 (MQ=255)
                        gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:604743/60‑1 (MQ=255)
                        gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:4243612/60‑1 (MQ=255)
                        gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:4065974/60‑1 (MQ=255)
                        gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:3181459/60‑1 (MQ=255)
                        gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:4249105/60‑1 (MQ=255)
                        gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:5145098/60‑1 (MQ=255)
                        gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:1016608/60‑1 (MQ=255)
                        gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:5126352/60‑1 (MQ=255)
                        gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:2687033/60‑1 (MQ=255)
                        gTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:1972309/60‑1 (MQ=255)
                         ttACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaa                                                          >  1:4008616/1‑49 (MQ=255)
                         ttACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaaa                                                          >  1:4206381/1‑49 (MQ=255)
                         ttACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat                                             <  1:2845615/62‑1 (MQ=255)
                               aTAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgt                              <  1:3294772/71‑1 (MQ=255)
                                tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAGATAAAAAAACGTGAAATTATTATGCCGCCAGGCgta                             <  1:1707430/71‑1 (MQ=255)
                                tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAGATAAAAAAACGTGAAATTATTATGCCGCCAGGCgta                             <  1:138827/71‑1 (MQ=255)
                                tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaa                                                           <  1:1033291/41‑1 (MQ=255)
                                tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaa                                                           <  1:3755698/41‑1 (MQ=255)
                                tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATaaaa                                                           <  1:5077843/41‑1 (MQ=255)
                                tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatt                                               <  1:4806820/53‑1 (MQ=255)
                                tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgt                              <  1:1348487/70‑1 (MQ=255)
                                tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgt                              <  1:4717199/70‑1 (MQ=255)
                                tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgt                              <  1:4737265/70‑1 (MQ=255)
                                tAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgt                              <  1:4239181/70‑1 (MQ=255)
                                 aaCGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatt                                               <  1:4540138/52‑1 (MQ=255)
                                 aaCGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatt                                               <  1:434041/52‑1 (MQ=255)
                                 aaCGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatt                                               <  1:3682453/52‑1 (MQ=255)
                                 aaCGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAatt                                               <  1:402908/52‑1 (MQ=255)
                                  aCGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtag                            <  1:2716918/70‑1 (MQ=255)
                                  aCGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtag                            >  1:2675614/1‑70 (MQ=255)
                                   cGTGATGTGCCTTGTAATTTTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta                          >  1:4991813/1‑71 (MQ=255)
                                   cGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta                          >  1:4753611/1‑71 (MQ=255)
                                   cGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta                          >  1:2979143/1‑71 (MQ=255)
                                   cGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta                          >  1:265368/1‑71 (MQ=255)
                                   cGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta                          >  1:4941890/1‑71 (MQ=255)
                                   cGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta                          >  1:1080344/1‑71 (MQ=255)
                                   cGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCgtagta                          >  1:1279491/1‑71 (MQ=255)
                                         gtgCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCa                                   >  1:2907536/1‑56 (MQ=255)
                                         gtgCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCa                                   >  1:4529668/1‑56 (MQ=255)
                                            ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGaaa                                                 >  1:4584632/1‑39 (MQ=255)
                                            ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:4354548/40‑1 (MQ=255)
                                            ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:1366429/40‑1 (MQ=255)
                                            ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:1163407/40‑1 (MQ=255)
                                            ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:1119017/40‑1 (MQ=255)
                                            ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:678225/40‑1 (MQ=255)
                                            ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAat                                                <  1:1779378/40‑1 (MQ=255)
                                            ccTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGcagcag                 >  1:4064281/1‑71 (MQ=255)
                                              ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat                                             <  1:1862693/41‑1 (MQ=255)
                                              ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat                                             <  1:2780580/41‑1 (MQ=255)
                                              ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat                                             <  1:87057/41‑1 (MQ=255)
                                              ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat                                             <  1:2157384/41‑1 (MQ=255)
                                              ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat                                             <  1:3673219/41‑1 (MQ=255)
                                              ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat                                             <  1:1762335/41‑1 (MQ=255)
                                              ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAattat                                             <  1:391511/41‑1 (MQ=255)
                                              ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATgc                                        <  1:2569547/46‑1 (MQ=255)
                                              ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATgc                                        <  1:214146/46‑1 (MQ=255)
                                              ttGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATgc                                        <  1:647330/46‑1 (MQ=255)
                                                gTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATgccg                                      >  1:1971149/1‑46 (MQ=255)
                                                      cTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAgatga        <  1:37523/70‑1 (MQ=255)
                                                       ttATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTAt                         >  1:1571980/1‑52 (MQ=255)
                                                       ttATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTAt                         >  1:928593/1‑52 (MQ=255)
                                                          tCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAg   <  1:969334/71‑1 (MQ=255)
                                                           cAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCg                               <  1:352244/42‑1 (MQ=255)
                                                            aGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAgg  <  1:4852988/70‑1 (MQ=255)
                                                            aGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAgg  <  1:804493/70‑1 (MQ=255)
                                                                  |                                                               
GTCAGAAAATTGTTACATTACTATGTTACGCATAACGTGATGTGCCTTGTAATTCTTATCAGTAGAAATAAAAAAACGTGAAATTATTATGCCGCCAGGCGTAGTATCGCAGCAGGTAAGATGATTCAGG  >  Exported/2935868‑2935997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: