Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,965,095 G→T 6.7% A193S (GCG→TCG)  deoA → thymidine phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,965,0950GT 6.7% 343.7 / 5.6 101A193S (GCG→TCG) deoAthymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (37/57);  new base T (4/3);  total (41/60)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGA  >  Exported/2965036‑2965162
                                                           |                                                                   
ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                          <  1:2187430/71‑1 (MQ=255)
ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                          <  1:3981556/71‑1 (MQ=255)
ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                          <  1:4275318/71‑1 (MQ=255)
ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                          <  1:4556448/71‑1 (MQ=255)
ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                          <  1:172204/71‑1 (MQ=255)
  aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                                         <  1:5040416/70‑1 (MQ=255)
  aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                                         <  1:3606100/70‑1 (MQ=255)
  aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                                         <  1:346309/70‑1 (MQ=255)
   ccGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAc                                                       >  1:4813304/1‑71 (MQ=255)
      cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                                    >  1:248198/1‑71 (MQ=255)
      cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                                    >  1:3720867/1‑71 (MQ=255)
      cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                                    >  1:4779475/1‑71 (MQ=255)
      cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgc                                                     >  1:3699205/1‑70 (MQ=255)
      cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcg                                                      >  1:5087612/1‑69 (MQ=255)
        aCCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCt                                                  <  1:2012054/71‑1 (MQ=255)
            tgtaCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgc                                                     <  1:4562786/61‑1 (MQ=255)
            tGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGtt                                              <  1:2211737/71‑2 (MQ=255)
            tGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTg                                              <  1:1541262/71‑1 (MQ=255)
               aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                           <  1:1747669/71‑1 (MQ=255)
               aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                           <  1:4317285/71‑1 (MQ=255)
               aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                           <  1:2133124/71‑1 (MQ=255)
               aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                           <  1:3661095/71‑1 (MQ=255)
               aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                           <  1:3819659/71‑1 (MQ=255)
                   cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGAc                                        >  1:726518/1‑70 (MQ=255)
                   cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACg                                       >  1:2423087/1‑71 (MQ=255)
                   cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGCAAGGTCTGGa                                                        >  1:4767651/1‑54 (MQ=255)
                       ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                                    <  1:2422454/70‑1 (MQ=255)
                       ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                                    <  1:933279/70‑1 (MQ=255)
                       ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                                    <  1:748009/70‑1 (MQ=255)
                       ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                                    <  1:749094/70‑1 (MQ=255)
                           tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                               >  1:5139499/1‑70 (MQ=255)
                           tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                               >  1:3765659/1‑70 (MQ=255)
                           tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                               >  1:627910/1‑70 (MQ=255)
                           tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                               >  1:4654633/1‑70 (MQ=255)
                            gATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTgg                              >  1:2511946/1‑71 (MQ=255)
                               cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                                >  1:5088838/1‑50 (MQ=255)
                               cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                                >  1:509576/1‑50 (MQ=255)
                               cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                                >  1:542705/1‑50 (MQ=255)
                                 ccGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgc                         <  1:1564036/71‑1 (MQ=255)
                                 ccGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgc                         <  1:1572932/71‑1 (MQ=255)
                                  cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg                        <  1:5096278/68‑1 (MQ=255)
                                  cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg                        <  1:5072468/68‑1 (MQ=255)
                                  cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg                        <  1:4421560/68‑1 (MQ=255)
                                    ccTCTATTCTGGCGAAGAAACTAGCGGAAGGACTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgcggc                      >  1:1677587/1‑71 (MQ=255)
                                      tctATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:5198074/1‑57 (MQ=255)
                                        tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                               <  1:1698747/42‑1 (MQ=255)
                                        tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                               <  1:3360109/42‑1 (MQ=255)
                                        tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                               <  1:3353852/42‑1 (MQ=255)
                                        tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                               <  1:1788533/42‑1 (MQ=255)
                                        tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGt                  >  1:3394182/1‑71 (MQ=255)
                                        tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGt                  >  1:3431812/1‑71 (MQ=255)
                                          ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                                         <  1:3111659/46‑1 (MQ=255)
                                          ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                                         <  1:4703971/46‑1 (MQ=255)
                                          ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                                         <  1:2363989/46‑1 (MQ=255)
                                          ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGtt                 <  1:4676491/70‑1 (MQ=255)
                                           tCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                                >  1:752776/1‑38 (MQ=255)
                                           tCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                                >  1:3567664/1‑38 (MQ=255)
                                            cTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               >  1:3790149/1‑70 (MQ=255)
                                               gCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTAt              <  1:288183/68‑1 (MQ=255)
                                               gCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATg             <  1:3369350/69‑1 (MQ=255)
                                                cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               >  1:1118185/1‑66 (MQ=255)
                                                cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               >  1:1580525/1‑66 (MQ=255)
                                                cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               >  1:2190962/1‑66 (MQ=255)
                                                cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               >  1:4314104/1‑66 (MQ=255)
                                                cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGAGCGTTTa               >  1:1894959/1‑66 (MQ=255)
                                                 gaagaaACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               <  1:3469375/65‑1 (MQ=255)
                                                 gaagaaACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               <  1:3338671/65‑1 (MQ=255)
                                                     aaaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGtt                 <  1:414006/59‑1 (MQ=255)
                                                     aaaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGtt                 <  1:1211473/59‑1 (MQ=255)
                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:3793051/1‑41 (MQ=255)
                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:2364113/1‑41 (MQ=255)
                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:1840212/1‑41 (MQ=255)
                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:1756997/1‑41 (MQ=255)
                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:3816621/1‑41 (MQ=255)
                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:1272662/1‑41 (MQ=255)
                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:443967/1‑41 (MQ=255)
                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:342745/1‑41 (MQ=255)
                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:4293125/1‑41 (MQ=255)
                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTAc    >  1:2660731/1‑71 (MQ=255)
                                                       aCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTAc    >  1:1433811/1‑70 (MQ=255)
                                                       aCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTAc    >  1:4251476/1‑70 (MQ=255)
                                                        cTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGttt                <  1:1124774/57‑1 (MQ=255)
                                                        cTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGttt                <  1:428748/57‑1 (MQ=255)
                                                        cTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGttt                <  1:2411933/57‑1 (MQ=255)
                                                        cTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGa  <  1:3479313/71‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTTGTGATGGACGTg                                     <  1:4682206/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:2613051/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:50677/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:5025086/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:497479/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:3934055/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:2117419/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:2817060/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:477423/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:1115289/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:2994071/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:1561257/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:4688227/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:3183666/35‑1 (MQ=255)
                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:3367068/35‑1 (MQ=255)
                                                           |                                                                   
TTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGA  >  Exported/2965036‑2965162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: