Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 710,033 G→C 18.7% intergenic (‑159/+102) yceJ ← / ← yceO predicted cytochrome b561/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported710,0330GC18.7% 109.9 / 17.8 48intergenic (‑159/+102)yceJ/yceOpredicted cytochrome b561/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (16/23);  new base C (4/5);  total (20/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGA  >  Exported/709972‑710098
                                                             |                                                                 
ttCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCt                                                             <  1:2006252/68‑1 (MQ=255)
 tCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCt                                                             <  1:1880417/67‑1 (MQ=255)
   aataatTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGa                                                        >  1:2126073/1‑70 (MQ=255)
   aataatTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAg                                                       >  1:2796381/1‑71 (MQ=255)
       atTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGc                                                    <  1:5112651/70‑1 (MQ=255)
       atTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCt                                                   <  1:4283841/71‑1 (MQ=255)
               ttAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGt                                                 <  1:3374947/65‑1 (MQ=255)
               ttAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGt                                                 <  1:2782867/65‑1 (MQ=255)
                  ataatGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg                                        <  1:3901896/71‑1 (MQ=255)
                  ataatGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg                                        <  1:2925708/71‑1 (MQ=255)
                  ataatGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg                                        <  1:2612838/71‑1 (MQ=255)
                       gAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGaataa                                   >  1:1986808/1‑71 (MQ=255)
                        aaaGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGaata                                    >  1:4771741/1‑69 (MQ=255)
                          aGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGt                                                          <  1:3233958/45‑1 (MQ=255)
                          aGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGt                                                          <  1:4497772/45‑1 (MQ=255)
                            aaTCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGaa                                      <  1:1031990/63‑1 (MQ=255)
                                ttCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGt                                                 <  1:212990/48‑1 (MQ=255)
                                   aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtt                       <  1:2517586/71‑1 (MQ=255)
                                   aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtt                       >  1:3057822/1‑71 (MQ=255)
                                   aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtt                       >  1:1948864/1‑71 (MQ=255)
                                     aaGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg                                        <  1:3654907/52‑1 (MQ=255)
                                     aaGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtttt                     >  1:4267481/1‑71 (MQ=255)
                                      aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAttttt                    >  1:3919562/1‑71 (MQ=255)
                                      aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAttttt                    >  1:2999409/1‑71 (MQ=255)
                                      aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAttttt                    <  1:1171786/71‑1 (MQ=255)
                                      aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtttt                     >  1:4025461/1‑70 (MQ=255)
                                        tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa                  <  1:4179995/71‑1 (MQ=255)
                                        tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa                  <  1:2357741/71‑1 (MQ=255)
                                        tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa                  <  1:1497317/71‑1 (MQ=255)
                                        tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa                  <  1:1035620/71‑1 (MQ=255)
                                                  tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt                        >  1:3048406/1‑55 (MQ=255)
                                                  tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt                        >  1:3478854/1‑55 (MQ=255)
                                                  tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt                        >  1:3607620/1‑55 (MQ=255)
                                                  tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt                        >  1:5026125/1‑55 (MQ=255)
                                                    aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTACATGaaa      <  1:672418/71‑1 (MQ=255)
                                                    aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGaaa      <  1:4633094/71‑1 (MQ=255)
                                                    aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGaaa      <  1:999210/71‑1 (MQ=255)
                                                      cATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg    >  1:4060223/1‑71 (MQ=255)
                                                       accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg    >  1:3190029/3‑70 (MQ=255)
                                                       accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg    >  1:3009245/3‑70 (MQ=255)
                                                       accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg    >  1:2914268/3‑70 (MQ=255)
                                                       accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAg     >  1:2008248/3‑69 (MQ=255)
                                                        tCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg    >  1:4128032/1‑69 (MQ=255)
                                                        ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga  <  1:4337496/70‑1 (MQ=255)
                                                        ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga  <  1:4623873/70‑1 (MQ=255)
                                                        ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga  <  1:1123524/70‑1 (MQ=255)
                                                        ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTACACGCTAAATGAAAGGga  <  1:4253674/70‑1 (MQ=255)
                                                        ccAGACATCTCTGATCAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga  <  1:4721577/70‑1 (MQ=255)
                                                          agagATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg    >  1:1713701/1‑67 (MQ=255)
                                                          agagATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg    >  1:17136/1‑67 (MQ=255)
                                                             gaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACg                >  1:519359/1‑52 (MQ=255)
                                                             gaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACg                >  1:1345833/1‑52 (MQ=255)
                                                             |                                                                 
TTCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGA  >  Exported/709972‑710098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: