| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 775,580 | A→G | 8.8% | K401K (AAA→AAG) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 775,580 | 0 | A | G | 8.8% | 226.3 / 10.5 | 79 | K401K (AAA→AAG) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base A (41/31); new base G (4/3); total (45/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
AAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATT > Exported/775510‑775647 | aaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa < 1:681110/71‑1 (MQ=255)aaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:2830966/1‑71 (MQ=255)aaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:4691561/1‑71 (MQ=255)aaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:3765108/1‑71 (MQ=255) tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAAc < 1:939983/66‑1 (MQ=255) tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAAc < 1:933888/66‑1 (MQ=255) tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAAc < 1:1772275/66‑1 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa > 1:989281/1‑70 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa > 1:4658591/1‑70 (MQ=255) aGGCGCAATCAACTCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa < 1:4160267/70‑1 (MQ=255) cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa > 1:2391016/1‑67 (MQ=255) cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa > 1:1779641/1‑67 (MQ=255) cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa > 1:1774478/1‑67 (MQ=255) cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa > 1:488470/1‑67 (MQ=255) gcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGtt > 1:2005204/1‑70 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgctg > 1:1296442/1‑59 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaa > 1:1932466/1‑61 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag > 1:1525222/1‑71 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag > 1:1519065/1‑71 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag > 1:1008689/1‑71 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag > 1:2974269/1‑71 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag > 1:2968105/1‑71 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:4067391/1‑52 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:3421183/1‑52 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:4112656/1‑52 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:4116269/1‑52 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:3518763/1‑52 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:1581657/1‑52 (MQ=255) cAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGt > 1:3671804/1‑71 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGtt < 1:4146556/71‑1 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGtt < 1:2771650/71‑1 (MQ=255) cgAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:1640628/71‑1 (MQ=255) cgAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:4369187/71‑1 (MQ=255) cgAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:1192956/71‑1 (MQ=255) gAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:175640/70‑1 (MQ=255) aaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa < 1:4495973/50‑1 (MQ=255) aaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa < 1:3804028/50‑1 (MQ=255) aaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa < 1:1923476/50‑1 (MQ=255) aaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa < 1:4542498/50‑1 (MQ=255) aaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa < 1:2277545/50‑1 (MQ=255) ccGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg < 1:2662816/71‑1 (MQ=255) cGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGa < 1:1492228/71‑1 (MQ=255) tAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCag < 1:1264457/54‑1 (MQ=255) tAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCag < 1:952432/54‑1 (MQ=255) tATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTg > 1:2575260/1‑58 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAc < 1:2220263/71‑1 (MQ=255) cTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:2091709/52‑1 (MQ=255) ttCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAc < 1:200760/69‑1 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:1036730/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:4307537/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:4670839/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:615486/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:920139/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:3838587/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:2324348/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTAAGAGTTTGGTGACGCGATCATCGaaaa > 1:702098/1‑66 (MQ=255) gAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:2675509/70‑1 (MQ=255) gAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:530199/70‑1 (MQ=255) gAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:234235/70‑1 (MQ=255) gAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGAGATCATCGAAAACATGt < 1:1174415/70‑1 (MQ=255) aGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:2489055/69‑1 (MQ=255) cTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTgg > 1:3645845/1‑41 (MQ=255) cTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa > 1:3653056/1‑44 (MQ=255) cTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa > 1:2289484/1‑44 (MQ=255) cTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa > 1:1048013/1‑44 (MQ=255) cTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa > 1:4473567/1‑44 (MQ=255) cTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa > 1:4747777/1‑44 (MQ=255) cTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa > 1:107080/1‑44 (MQ=255) cTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa > 1:4438996/1‑44 (MQ=255) atggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat > 1:2257935/1‑47 (MQ=255) atggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat > 1:1189648/1‑47 (MQ=255) atggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat > 1:3074056/1‑47 (MQ=255) ggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt > 1:4302522/1‑71 (MQ=255) ggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt > 1:4380685/1‑71 (MQ=255) ggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt > 1:2294506/1‑71 (MQ=255) ggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt > 1:1093829/1‑71 (MQ=255) ggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt > 1:3367555/1‑71 (MQ=255) ggaaggCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaaaa < 1:3802820/54‑1 (MQ=255) ggaaggCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaaaa < 1:3796755/54‑1 (MQ=255) gatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGcc < 1:5063702/64‑1 (MQ=255) gatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGcc < 1:4664684/64‑1 (MQ=255) atggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGcc < 1:4066564/63‑1 (MQ=255) aaggCGCTAAGCTTCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACa > 1:3348691/3‑54 (MQ=255) aaggCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACa > 1:812079/3‑54 (MQ=255) aaggCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACa > 1:4743693/3‑54 (MQ=255) aaggCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACa > 1:3927844/3‑54 (MQ=255) gcgcTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTa < 1:3009855/71‑1 (MQ=255) cgcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaaaa < 1:2074765/48‑1 (MQ=39) cTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAt > 1:4467167/1‑71 (MQ=255) aaCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt > 1:4449925/1‑69 (MQ=255) aaCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt < 1:4193328/69‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCCTCGaaa < 1:668113/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCCTCGaaa < 1:3557/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:5092064/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:4806127/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:3043059/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:1259462/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:3154665/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:3963061/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:1595957/41‑1 (MQ=255) | AAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATT > Exported/775510‑775647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |