Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,319,256 G→A 11.2% N212N (AAC→AAT ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,319,2560GA11.2% 301.4 / 21.7 107N212N (AAC→AATugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (34/61);  new base A (5/7);  total (39/68)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

GTCGAGACAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTG  >  Exported/1319187‑1319322
                                                                     |                                                                  
gTCGAGACAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGt                                                                   >  1:1162301/1‑71 (MQ=255)
   gagaCAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTa                                                                 <  1:4216947/70‑1 (MQ=255)
   gagaCAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTa                                                                 <  1:2511821/70‑1 (MQ=255)
   gagaCAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTa                                                                 <  1:3609961/70‑1 (MQ=255)
     gaCAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAg                                                              >  1:527932/1‑71 (MQ=255)
     gaCAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAg                                                              <  1:643484/71‑1 (MQ=255)
     gaCAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAg                                                              <  1:839762/71‑1 (MQ=255)
        aaaCGCCTTCGTTTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTAc                                                           <  1:368695/71‑1 (MQ=255)
        aaaCGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTAc                                                           <  1:848508/71‑1 (MQ=255)
         aaCGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTAc                                                           <  1:1222991/70‑1 (MQ=255)
         aaCGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACg                                                          >  1:3739270/1‑71 (MQ=255)
                tCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCAcgcg                                                   >  1:5151672/1‑71 (MQ=255)
                tCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCAcgcg                                                   >  1:1831661/1‑71 (MQ=255)
                 cGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCAcg                                                     <  1:688905/68‑1 (MQ=255)
                 cGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCAcg                                                     <  1:2207435/68‑1 (MQ=255)
                 cGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCAcg                                                     <  1:4319808/68‑1 (MQ=255)
                   attattTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTa                                                                 <  1:4570215/54‑1 (MQ=255)
                   attattTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTa                                                                 <  1:792123/54‑1 (MQ=255)
                   attattTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTa                                                                 <  1:4746644/54‑1 (MQ=255)
                      attTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTaa                                                                <  1:374708/52‑1 (MQ=255)
                      attTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTaa                                                                <  1:519662/52‑1 (MQ=255)
                       tttGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGcc                                            <  1:3682266/71‑1 (MQ=255)
                       tttGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGcc                                            <  1:3291584/71‑1 (MQ=255)
                       tttGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGcc                                            <  1:3166188/71‑1 (MQ=255)
                       tttGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGcc                                            <  1:2953184/71‑1 (MQ=255)
                       tttGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGcc                                            <  1:2695921/71‑1 (MQ=255)
                        ttGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGc                                             >  1:3710757/1‑69 (MQ=255)
                               gAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt                                    >  1:4439288/1‑71 (MQ=255)
                               gAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt                                    >  1:2953928/1‑71 (MQ=255)
                                  ttCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGtt                                 <  1:3818423/71‑1 (MQ=255)
                                   tCAGACCAAAACTTTCTGCGTAACTATCCAATTCATTAAAAAATGCAACTCGCATAGCCaa                                          >  1:4607805/1‑60 (MQ=255)
                                   tCAGACCAAAACTTTCTGAGTAACTATCCAATTCATTAAAAAATGCAACTCGCATAGCCaa                                          >  1:4370253/1‑60 (MQ=255)
                                   tCAGACCAAAACTTTCTGAGTAACTATCCAATTCATTAAAAAATGCAACTCGCATAGCCaa                                          >  1:2217858/1‑60 (MQ=255)
                                   tCAGACCAAAACTTTCTGAGTAACTATCCAATTCATTAAAAAATGCAACTCGCATAGCCaa                                          >  1:4681044/1‑60 (MQ=255)
                                        ccTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCaaa                           <  1:690645/71‑1 (MQ=255)
                                        ccTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCaaa                           <  1:5170976/71‑1 (MQ=255)
                                           aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCAc                                                      >  1:443891/1‑41 (MQ=255)
                                           aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCAt                                                >  1:1986865/1‑47 (MQ=255)
                                           aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCAt                                                >  1:2010709/1‑47 (MQ=255)
                                           aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCAt                                                >  1:1373500/1‑47 (MQ=255)
                                           aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCAt                                                >  1:3581539/1‑47 (MQ=255)
                                           aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGttt                                >  1:1921521/1‑63 (MQ=255)
                                           aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGttt                                >  1:3589920/1‑63 (MQ=255)
                                           aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGttt                                >  1:2684495/1‑63 (MQ=255)
                                           aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGttt                                >  1:610546/1‑63 (MQ=255)
                                           aaaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGACAGGTAGGTGTTTGCaa                            >  1:2159600/1‑67 (MQ=255)
                                            aaCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGt                       >  1:4960402/1‑71 (MQ=255)
                                             aCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGtt                      >  1:2494079/1‑71 (MQ=255)
                                             aCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGtt                      >  1:4884383/1‑71 (MQ=255)
                                             aCTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGtt                      >  1:1482459/1‑71 (MQ=255)
                                              cTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGtt                      >  1:2909430/1‑70 (MQ=255)
                                               tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt                                    <  1:3768706/55‑1 (MQ=255)
                                               tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt                                    <  1:1501587/55‑1 (MQ=255)
                                               tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt                                    <  1:1103894/55‑1 (MQ=255)
                                               tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt                                    <  1:4812659/55‑1 (MQ=255)
                                               tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt                                    <  1:1840174/55‑1 (MQ=255)
                                               tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt                                    <  1:582410/55‑1 (MQ=255)
                                               tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt                                    <  1:1932202/55‑1 (MQ=255)
                                               tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt                                    <  1:1234297/55‑1 (MQ=255)
                                               tttCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggtaggt                                    <  1:3383016/55‑1 (MQ=255)
                                                 tCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa                    <  1:649933/69‑1 (MQ=255)
                                                 tCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa                    <  1:1005338/69‑1 (MQ=255)
                                                 tCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa                    <  1:3315514/69‑1 (MQ=255)
                                                 tCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa                    <  1:2043141/69‑1 (MQ=255)
                                                 tCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa                    <  1:3252656/69‑1 (MQ=255)
                                                 tCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa                    <  1:3203773/69‑1 (MQ=255)
                                                  cTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTa                    <  1:5011502/68‑1 (MQ=255)
                                                     cATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGc               <  1:4969544/70‑1 (MQ=255)
                                                         gCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTg          <  1:1661509/71‑1 (MQ=255)
                                                         acTATCCAATTCATTAAAAAATGCAACTCGCATAGCCAAATAAGTATTTGAAAATAACTTAATCGCTTccg          <  1:615495/70‑3 (MQ=255)
                                                         acTATCCAATTCATTAAAAAATGCAACTCGCATAGCCAAATAAGTATTTGAAAATAACTTAATCGCTTccg          <  1:4152400/70‑3 (MQ=255)
                                                         acTATCCAATTCATTAAAAAATGCAACTCGCATAGCCAAATAAGTATTTGAAAATAACTTAATCGCTTccg          <  1:4724149/70‑3 (MQ=255)
                                                         acTATCCAATTCATTAAAAAATGCAACTCGCATAGCCAAATAAGTATTTGAAAATAACTTAATCGCTTccg          <  1:1720232/70‑3 (MQ=255)
                                                         acTATCCAATTCATTAAAAAATGCAACTCGCATAGCCAAATAAGTATTTGAAAATAACTTAATCGCTTccg          <  1:3078896/70‑3 (MQ=255)
                                                         acTATCCAATTCATTAAAAAATGCAACTCGCATAGCCAAATAAGTATTTGAAAATAACTTAATCGCTTccg          <  1:755333/70‑3 (MQ=255)
                                                         acTATCCAATTCATTAAAAAATGCAACTCGCATAGCCAAATAAGTATTTGAAAATAACTTAATCGCTTccg          <  1:2299047/70‑3 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:1215767/38‑1 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:3055388/38‑1 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:3388996/38‑1 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:3452786/38‑1 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:4966096/38‑1 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:899588/38‑1 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:4947300/38‑1 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:4846742/38‑1 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:399514/38‑1 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:146121/38‑1 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:4213133/38‑1 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:4600452/38‑1 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:4576582/38‑1 (MQ=255)
                                                             tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCaggta                                       <  1:4524427/38‑1 (MQ=255)
                                                                   tCGTTAGAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTg  >  1:3431117/1‑69 (MQ=255)
                                                                   tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTg                               >  1:62049/1‑40 (MQ=255)
                                                                   tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAAt                  >  1:902440/1‑53 (MQ=255)
                                                                   tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAAt                  >  1:4666664/1‑53 (MQ=255)
                                                                   tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAAt                  >  1:4340832/1‑53 (MQ=255)
                                                                   tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAAt                  >  1:370886/1‑53 (MQ=255)
                                                                   tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAAt                  >  1:1623029/1‑53 (MQ=255)
                                                                   tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTg          >  1:4897004/1‑61 (MQ=255)
                                                                   tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGt   >  1:5005039/1‑68 (MQ=255)
                                                                   tCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTg  >  1:1084515/1‑69 (MQ=255)
                                                                     |                                                                  
GTCGAGACAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTG  >  Exported/1319187‑1319322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: