| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,393,077 | T→C | 6.1% | K241E (AAA→GAA) | fruK ← | fructose‑1‑phosphate kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,393,077 | 0 | T | C | 6.1% | 432.7 / 13.5 | 146 | K241E (AAA→GAA) | fruK | fructose‑1‑phosphate kinase |
| Reads supporting (aligned to +/- strand): ref base T (56/81); new base C (7/2); total (63/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.96e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
Only 100 of 154 total aligned reads displayed.
CCATAAATCAGGCCACCAACCATAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGC > Exported/1393007‑1393146 | ccATAAATCAGGCCACCAACCATAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGttt > 1:2797416/1‑71 (MQ=255)ccATAAATCAGGCCACCAACCATAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGttt > 1:3525508/1‑71 (MQ=255)ccATAAATCAGGCCACCAACCATAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGttt > 1:3631226/1‑71 (MQ=255)ccATAAATCAGGCCACCAACCATAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGttt > 1:2347833/1‑71 (MQ=255) aTAAATCAGGCCACCAACCATAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTgg > 1:4596485/1‑71 (MQ=255) tAAATCAGGCCACCAACCATAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGTTTTGGc > 1:3101409/1‑71 (MQ=255) tAAATCAGGCCACCAACCATAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTgg > 1:2109352/1‑70 (MQ=255) tAAATCAGGCCACCAACCATAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGc > 1:4654118/1‑71 (MQ=255) ccaccaACCATAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTc < 1:4210631/71‑1 (MQ=255) accaaccaTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGc < 1:603644/71‑1 (MQ=255) ccaaccaTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGc < 1:4635071/70‑1 (MQ=255) ccaTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATc < 1:589176/59‑1 (MQ=255) ccaTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATc < 1:314657/59‑1 (MQ=255) ccaTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATc < 1:3666499/59‑1 (MQ=255) ccaTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATc < 1:3783427/59‑1 (MQ=255) ccaTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATc < 1:592686/59‑1 (MQ=255) ccaTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATc < 1:2646270/59‑1 (MQ=255) ccaTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATc < 1:1958279/59‑1 (MQ=255) ccaTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATc < 1:1506720/59‑1 (MQ=255) caTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCga < 1:3694710/65‑2 (MQ=255) caTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCga < 1:345212/65‑2 (MQ=255) caTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAgg > 1:5030355/1‑71 (MQ=255) aTAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTc < 1:2439363/62‑1 (MQ=255) tAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCa > 1:4245057/1‑71 (MQ=255) gAATCCCCTGCGCCAACGGGGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCAtt < 1:5154479/71‑1 (MQ=255) aTCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGATGCATTa < 1:3916/70‑1 (MQ=255) aTCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTa < 1:194781/70‑1 (MQ=255) aTCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTa < 1:2859749/70‑1 (MQ=255) aTCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTa < 1:1223198/70‑1 (MQ=255) tCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAAc > 1:1699899/1‑71 (MQ=255) cccTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAAcc < 1:2154469/70‑1 (MQ=255) ccTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATc < 1:40305/48‑1 (MQ=255) cTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCaa < 1:857177/71‑1 (MQ=255) cTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCaa < 1:1612668/71‑1 (MQ=255) cTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCaa < 1:3230493/71‑1 (MQ=255) cTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCaa < 1:4073867/71‑1 (MQ=255) tGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCa > 1:920153/1‑69 (MQ=255) tGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCa > 1:5170120/1‑69 (MQ=255) tGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCa > 1:894387/1‑69 (MQ=255) tGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCa > 1:226197/1‑69 (MQ=255) cgcCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCaaat < 1:25073/70‑2 (MQ=255) cgcCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAg < 1:1737278/70‑1 (MQ=255) aaCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAgcgc > 1:1430703/1‑69 (MQ=255) aaCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAgcgc > 1:3698878/1‑69 (MQ=255) aaCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAgcgc > 1:3708149/1‑69 (MQ=255) aCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGc > 1:4968649/1‑46 (MQ=255) aCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGc > 1:2589344/1‑46 (MQ=255) aCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGc > 1:3519563/1‑46 (MQ=255) aCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGc > 1:2394383/1‑46 (MQ=255) aCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGc > 1:1543513/1‑46 (MQ=255) aCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGc > 1:2611945/1‑46 (MQ=255) aCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGc > 1:3994337/1‑46 (MQ=255) aCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGc > 1:3122097/1‑46 (MQ=255) aCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGc > 1:1302379/1‑46 (MQ=255) aCGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGcc > 1:81710/1‑71 (MQ=255) ggTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCg > 1:5021315/1‑46 (MQ=255) ggTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCg > 1:1008004/1‑46 (MQ=255) ggTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCg > 1:4460478/1‑46 (MQ=255) ggTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCg > 1:3464439/1‑46 (MQ=255) ggTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCg > 1:3332937/1‑46 (MQ=255) ggTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCg > 1:1321674/1‑46 (MQ=255) ggTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCg > 1:300009/1‑46 (MQ=255) ggTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCtt < 1:530517/71‑1 (MQ=255) ggTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCtt < 1:4481692/71‑1 (MQ=255) ggTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCAATCGCCg > 1:1750595/1‑46 (MQ=255) ggTGCTTACGACATAGACTGACGGTGGTTTGGCGATCCATTCGACg > 1:2308233/1‑46 (MQ=255) gTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCaa < 1:3591700/60‑1 (MQ=255) gTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCaa < 1:2601774/60‑1 (MQ=255) gTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCaa < 1:1206976/60‑1 (MQ=255) gTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCaa < 1:1866810/60‑1 (MQ=255) gTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTc < 1:2573257/71‑1 (MQ=255) ttACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCAtt < 1:281149/49‑1 (MQ=255) ttACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCaa < 1:4964860/56‑1 (MQ=255) tACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCAc > 1:3788900/1‑71 (MQ=255) aCGACATCGACTGACGGTGGTTTGGCGATCCATTc > 1:2511495/1‑35 (MQ=255) cGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCAcc > 1:199385/1‑70 (MQ=255) cGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCAcc > 1:4785876/1‑70 (MQ=255) tCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCAt < 1:2869420/40‑1 (MQ=255) tCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTg > 1:2695013/1‑70 (MQ=255) tCGACTGACGGTGGTTCGGCGATCCATTCGCCGGAGGCATTAACCCaa < 1:2692032/48‑1 (MQ=255) gACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGaa < 1:2096149/70‑1 (MQ=255) gACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGaa < 1:686198/70‑1 (MQ=255) gACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCAATAACCCAAAGCGCGCCTTCGGCACCCAGTGaaa > 1:4705781/1‑71 (MQ=255) gACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCAATAACCCAAAGCGCGCCTTCGGCACCCAGTGaa > 1:1029073/1‑70 (MQ=255) gACTGACGGTGGTTCGGCGATCCATTCGCCGGAGGCATTAACCCAAAg > 1:4696845/1‑48 (MQ=255) gACTGACGGTGGTTCGGCGATCCATTCGCCGGAGGCATTAACCCAAAg > 1:39351/1‑48 (MQ=255) gACTGACGGTGGTTCGGCGATCCATTCGCCGGAGGCATTAACCCAAAg > 1:1399416/1‑48 (MQ=255) aCTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAAt < 1:1782409/71‑1 (MQ=255) aCTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAAt < 1:3233970/71‑1 (MQ=255) aCGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATaaca < 1:651475/71‑1 (MQ=255) aCGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATaaca < 1:3955297/71‑1 (MQ=255) aCGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATaaca < 1:4927808/71‑1 (MQ=255) aCGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATaaca < 1:4227836/71‑1 (MQ=255) aCGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATaaca < 1:2747963/71‑1 (MQ=255) aCGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATaac > 1:5023751/1‑70 (MQ=255) cGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATaacaa < 1:5059162/71‑1 (MQ=255) cGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATaacaa < 1:886545/71‑1 (MQ=255) gtTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATg < 1:666084/70‑1 (MQ=255) tGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATgcgc < 1:2635612/70‑1 (MQ=255) tGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATgcgc > 1:1637217/1‑70 (MQ=255) | CCATAAATCAGGCCACCAACCATAGAATCCCCTGCGCCAACGGTGCTTACGACATCGACTGACGGTGGTTTGGCGATCCATTCGCCGGAGGCATTAACCCAAAGCGCGCCTTCGGCACCCAGTGAAATAACAACATGCGC > Exported/1393007‑1393146 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |