Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,403,006 G→A 11.1% P283P (CCG→CCA rtn → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,403,0060GA11.1% 201.4 / 12.1 72P283P (CCG→CCArtnconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (26/38);  new base A (3/5);  total (29/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTATGCGCCCCGGCAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCTGTCGC  >  Exported/1402938‑1403074
                                                                    |                                                                    
cgtaTGCGCCCCGGCAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCggt                                                                    <  1:3627315/71‑1 (MQ=255)
cgtaTGCGCCCCGGCAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCggt                                                                    <  1:3317174/71‑1 (MQ=255)
cgtaTGCGCCCCGGCAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCggt                                                                    <  1:1096032/71‑1 (MQ=255)
cgtaTGCGCCCCGGCAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCgg                                                                     <  1:4977704/70‑1 (MQ=255)
 gtaTGCGCCCCGGCAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCggtg                                                                   >  1:4174843/1‑71 (MQ=255)
 gtaTGCGCCCCGGCAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCggt                                                                    <  1:4774477/70‑1 (MQ=255)
  taTGCGCCCCGGCAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCggt                                                                    <  1:2191499/69‑1 (MQ=255)
          ccGGCAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGgtggtg                                                                <  1:574574/65‑1 (MQ=255)
              cAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAg                                                      <  1:4253409/71‑1 (MQ=255)
              cAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAg                                                      <  1:2204794/71‑1 (MQ=255)
              cAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAg                                                      <  1:1534895/71‑1 (MQ=255)
                 agaAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCtt                                                   <  1:3889773/71‑1 (MQ=255)
                 agaAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCtt                                                   <  1:1054480/71‑1 (MQ=255)
                   aaaTCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCttt                                                  >  1:495854/1‑70 (MQ=255)
                     aTCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCg                                               <  1:2995897/71‑1 (MQ=255)
                        aTGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATa                                                            >  1:379091/1‑55 (MQ=255)
                        aTGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATa                                                            >  1:2196589/1‑55 (MQ=255)
                        aTGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATa                                                            >  1:4771263/1‑55 (MQ=255)
                        aTGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATa                                                            >  1:4065317/1‑55 (MQ=255)
                        aTGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATa                                                            >  1:1544569/1‑55 (MQ=255)
                        aTGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATa                                                            >  1:5003617/1‑55 (MQ=255)
                        aTGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATa                                                            >  1:1804350/1‑55 (MQ=255)
                        aTGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGt                                            <  1:3198088/71‑1 (MQ=255)
                         tGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTa                                           <  1:2791129/71‑1 (MQ=255)
                               ccATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGt                                            <  1:4314844/64‑1 (MQ=255)
                                   cAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTg                                  >  1:4562942/1‑70 (MQ=255)
                                   cAAGCGCGAACAATTTTACGTGGCGTATCAACCGGAGGTGGATACACAAGCTTTGCGAGTAACGGGCCTg                                  >  1:752035/1‑70 (MQ=255)
                                    aaGCGCGAACAATTTTACGTGGCGTATCAACCggt                                                                    >  1:3430590/1‑35 (MQ=255)
                                    aaGCGCGAACAATTTTACGTGGCGTATCAACCggt                                                                    >  1:3125379/1‑35 (MQ=255)
                                    aaGCGCGAACAATTTTACGTGGCGTATCAACCggt                                                                    >  1:3688920/1‑35 (MQ=255)
                                    aaGCGCGAACAATTTTACGTGGCGTATCAACCggt                                                                    >  1:5011282/1‑35 (MQ=255)
                                    aaGCGCGAACAATTTTACGTGGCGTATCAACCggt                                                                    >  1:2983237/1‑35 (MQ=255)
                                    aaGCGCGAACAATTTTACGTGGCGTATCAACCggt                                                                    >  1:2663261/1‑35 (MQ=255)
                                    aaGCGCGAACAATTTTACGTGGCGTATCAACCggt                                                                    >  1:1603420/1‑35 (MQ=255)
                                    aaGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACaa                                                       >  1:1909634/1‑48 (MQ=255)
                                    aaGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACaa                                                       >  1:135425/1‑48 (MQ=255)
                                    aaGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACaa                                                       >  1:1451334/1‑48 (MQ=255)
                                    aaGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTgg                                 <  1:3596213/70‑1 (MQ=255)
                                    aaGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTgg                                 <  1:2175419/70‑1 (MQ=255)
                                    aaGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTgg                                 <  1:4613217/70‑1 (MQ=255)
                                      gcgcGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGt                                            <  1:3199236/57‑1 (MQ=255)
                                      gcgcGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGt                                            <  1:849885/57‑1 (MQ=255)
                                      gcgcGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGt                                            <  1:4242453/57‑1 (MQ=255)
                                      gcgcGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGt                                            <  1:3268233/57‑1 (MQ=255)
                                      gcgcGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTgg                                 <  1:673048/68‑1 (MQ=255)
                                      gcgcGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAg                              <  1:65606/71‑1 (MQ=255)
                                      gcgcGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAg                              <  1:3562195/71‑1 (MQ=255)
                                      gcgcGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAg                              <  1:1678117/71‑1 (MQ=255)
                                      gcgcGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAg                              <  1:2207736/71‑1 (MQ=255)
                                      gcgcGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAg                              <  1:4045224/71‑1 (MQ=255)
                                      gcgcGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAg                              >  1:2411341/1‑71 (MQ=255)
                                        gcgAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCg                                               <  1:3556579/52‑1 (MQ=255)
                                          gAACAATTTTACGTGGCGTATCAACCAGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGaa                               <  1:1310645/66‑1 (MQ=255)
                                          gAACAATTTTACGTGGCGTATCAACCAGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGaa                               <  1:48263/66‑1 (MQ=255)
                                          gAACAATTTTACGTGGCGTATCAACCAGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGaa                               <  1:1521023/66‑1 (MQ=255)
                                          gAACAATTTTACGTGGCGTATCAACCAGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGaa                               <  1:3116252/66‑1 (MQ=255)
                                          gAACAATTTTACGTGGCGTATCAACCAGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGaa                               <  1:2404783/66‑1 (MQ=255)
                                           aaCAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGa                                <  1:3225301/64‑1 (MQ=255)
                                           aaCAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGa                                <  1:1141801/64‑1 (MQ=255)
                                           aaCAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGa                                <  1:3231414/64‑1 (MQ=255)
                                           aaCAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGa                                <  1:2106127/64‑1 (MQ=255)
                                            aCAATTTTACGTGGCGTATCAACCAGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGt                             >  1:1083832/1‑66 (MQ=255)
                                            aCAATTTTACGTGGCGTATCAACCAGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGt                             >  1:4105026/1‑66 (MQ=255)
                                            aCAATTTTACGTGGCGTATCAACCAGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGt                             >  1:2093229/1‑66 (MQ=255)
                                              aaTTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTAATGCTa                      <  1:1362081/71‑1 (MQ=255)
                                                 ttttCGTGGCGTATCAACCGGTGGTGGATACACAAGCtt                                                   <  1:1875356/39‑1 (MQ=255)
                                                 tttACGTGGCGTATCAACCGGTGGTGGATACACAAGCtt                                                   <  1:1082781/39‑1 (MQ=255)
                                                 tttACGTGGCGTATCAACCGGTGGTGGATACACAAGCtt                                                   <  1:1882995/39‑1 (MQ=255)
                                                 tttACGTGGCGTATCAACCGGTGGTGGATACACAAGCtt                                                   <  1:1991374/39‑1 (MQ=255)
                                                 tttACGTGGCGTATCAACCGGTGGTGGATACACAAGCtt                                                   <  1:2032159/39‑1 (MQ=255)
                                                 tttACGTGGCGTATCAACCGGTGGTGGATACACAAGCtt                                                   <  1:4055014/39‑1 (MQ=255)
                                                  ttACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCt                  >  1:2280601/1‑71 (MQ=255)
                                                  ttACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCt                  >  1:2066571/1‑71 (MQ=255)
                                                  ttACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCt                  >  1:4166317/1‑71 (MQ=255)
                                                                aaCCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCTGTc    >  1:1083942/1‑71 (MQ=255)
                                                                aaCCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCTGTc    >  1:3960750/1‑71 (MQ=255)
                                                                  tcGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCTGTcgc  <  1:2416753/70‑1 (MQ=255)
                                                                  ccGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCt       <  1:4244154/66‑1 (MQ=255)
                                                                  ccGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCt       <  1:3347513/66‑1 (MQ=255)
                                                                   cGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCTGt     >  1:2182327/1‑67 (MQ=255)
                                                                   cGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCTGt     >  1:3330409/1‑67 (MQ=255)
                                                                   cGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCTGt     >  1:4833148/1‑67 (MQ=255)
                                                                   cGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCTGt     >  1:1623946/1‑67 (MQ=255)
                                                                   cGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCTGt     >  1:3878200/1‑67 (MQ=255)
                                                                   cGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCTGt     >  1:4026532/1‑67 (MQ=255)
                                                                   cGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCTGt     >  1:391404/1‑67 (MQ=255)
                                                                    |                                                                    
CGTATGCGCCCCGGCAGAGAAATCATGACCGCCATCAAGCGCGAACAATTTTACGTGGCGTATCAACCGGTGGTGGATACACAAGCTTTGCGAGTAACGGGCCTGGAAGTACTGCTACGCTGGCGGCATCCTGTCGC  >  Exported/1402938‑1403074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: