Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,418,305 T→C 11.2% I85T (ATC→ACC)  narP → DNA‑binding response regulator in two‑component regulatory system with NarQ or NarX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,418,3050TC11.2% 218.0 / 15.8 80I85T (ATC→ACC) narPDNA‑binding response regulator in two‑component regulatory system with NarQ or NarX
Reads supporting (aligned to +/- strand):  ref base T (47/24);  new base C (2/7);  total (49/31)
Fisher's exact test for biased strand distribution p-value = 2.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ATGAAAGGTATGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTA  >  Exported/1418235‑1418368
                                                                      |                                                               
atGAAAGGTATGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATtattat                                                                 >  1:4497981/1‑71 (MQ=255)
   aaaGGTATGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCt                                                              <  1:5116688/71‑1 (MQ=255)
   aaaGGTATGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCt                                                              >  1:4628661/1‑71 (MQ=255)
      ggTATGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGAc                                                           <  1:2605039/71‑1 (MQ=255)
      ggTATGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGAc                                                           <  1:3967373/71‑1 (MQ=255)
      ggTATGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGAc                                                           <  1:1828706/71‑1 (MQ=255)
      ggTATGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGAc                                                           <  1:1923025/71‑1 (MQ=255)
      ggTATGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGCATGGCGTTACCGCGCAAATTATTATCCTGAc                                                           <  1:3746923/71‑1 (MQ=255)
       gTATGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGAcc                                                          >  1:137935/1‑71 (MQ=255)
       gTATGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGAcc                                                          >  1:1927751/1‑71 (MQ=255)
          tGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGt                                                        <  1:403208/70‑1 (MQ=255)
          tGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTa                                                       <  1:3184520/71‑1 (MQ=255)
          tGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTa                                                       <  1:3462563/71‑1 (MQ=255)
          tGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTa                                                       <  1:1973073/71‑1 (MQ=255)
          tGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTa                                                       <  1:4767457/71‑1 (MQ=255)
            aGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGAc                                                           <  1:2437163/65‑1 (MQ=255)
            aGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATc                                                     >  1:1817803/1‑71 (MQ=255)
            aGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATc                                                     >  1:5027320/1‑71 (MQ=255)
              tGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATcc                                                    >  1:596514/1‑70 (MQ=255)
              tGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATcc                                                    >  1:4589129/1‑70 (MQ=255)
                gCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGAt                                                 >  1:2923897/1‑71 (MQ=255)
                gCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGAt                                                 >  1:1685789/1‑71 (MQ=255)
                gCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGAt                                                 >  1:2132930/1‑71 (MQ=255)
                   tGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATcc                                                    <  1:685887/65‑1 (MQ=255)
                     gATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTc                                            >  1:857849/1‑71 (MQ=255)
                     gATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGAAAATTATTATCCTGACCGTATCAGATGCCTc                                            >  1:1901905/1‑71 (MQ=255)
                        aCTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:3215103/1‑48 (MQ=255)
                        aCTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:321018/1‑48 (MQ=255)
                        aCTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:4755876/1‑48 (MQ=255)
                        aCTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:4323131/1‑48 (MQ=255)
                        aCTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:4913690/1‑48 (MQ=255)
                        aCTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:305954/1‑48 (MQ=255)
                        aCTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:1656527/1‑48 (MQ=255)
                        aCTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:1572219/1‑48 (MQ=255)
                        aCTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:650647/1‑48 (MQ=255)
                        aCTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:3157737/1‑48 (MQ=255)
                        aCTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:242466/1‑48 (MQ=255)
                            tcAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:3631557/1‑44 (MQ=255)
                            tcAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:3435426/1‑44 (MQ=255)
                            tcAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATc                                                                >  1:4369217/1‑44 (MQ=255)
                            tcAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCg                                       <  1:4329696/69‑1 (MQ=255)
                            tcAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGa                                      >  1:1253046/1‑70 (MQ=255)
                            tcAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGa                                      >  1:1378938/1‑70 (MQ=255)
                            tcAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGAt                                     >  1:2480882/1‑71 (MQ=255)
                            tcAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGAt                                     >  1:830631/1‑71 (MQ=255)
                            tcAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGAt                                     >  1:4299230/1‑71 (MQ=255)
                              aaTGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGt                                   >  1:4097362/1‑71 (MQ=255)
                                      gcgcAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTgc                             <  1:4845108/69‑1 (MQ=255)
                                         cAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGa                        <  1:3943508/71‑1 (MQ=255)
                                           gggATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATa                      <  1:1406096/71‑1 (MQ=255)
                                             gATGGCGTTACCGCGCAAATTATTATCCTGACCGTATcc                                                    <  1:2552222/39‑1 (MQ=255)
                                             gATGGCGTTACCGCGCAAATTATTATCCTGACCGTATcc                                                    <  1:1056325/39‑1 (MQ=255)
                                             gATGGCGTTACCGCGCAAATTATTACCCTGACCGTATCCGATGCCTc                                            <  1:3764226/47‑1 (MQ=255)
                                             gATGGCGTTACCGCGCAAATTATTACCCTGACCGTATCCGATGCCTc                                            <  1:3919720/47‑1 (MQ=255)
                                             gATGGCGTTACCGCGCAAATTATTACCCTGACCGTATCCGATGCCTc                                            <  1:3898179/47‑1 (MQ=255)
                                             gATGGCGTTACCGCGCAAATTATTACCCTGACCGTATCCGATGCCTc                                            <  1:3714225/47‑1 (MQ=255)
                                             gATGGCGTTACCGCGCAAATTATTACCCTGACCGTATCCGATGCCTc                                            <  1:2563898/47‑1 (MQ=255)
                                             gATGGCGTTACCGCGCAAATTATTACCCTGACCGTATCCGATGCCTc                                            <  1:2569942/47‑1 (MQ=255)
                                             gATGGCGTTACCGCGCAAATTATTACCCTGACCGTATCCGATGCCTc                                            <  1:1179287/47‑1 (MQ=255)
                                               tGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACg                  <  1:2132597/71‑1 (MQ=255)
                                               tGGCGTTACCGCGCAAATTATTACCCTGACCGTATCCGATGCCTCCa                                          >  1:3873598/1‑47 (MQ=255)
                                               tGGCGTTACCGCGCAAATTATTACCCTGACCGTATCCGATGCCTCCa                                          >  1:5139273/1‑47 (MQ=255)
                                                 gCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACg                  >  1:944206/1‑69 (MQ=255)
                                                 gCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACg                  >  1:3716159/1‑69 (MQ=255)
                                                 gCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACg                  >  1:3523028/1‑69 (MQ=255)
                                                 gCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCa                <  1:4187874/71‑1 (MQ=255)
                                                 gCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCa                <  1:3206246/71‑1 (MQ=255)
                                                      aCCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGgcg            >  1:2274603/1‑70 (MQ=255)
                                                       ccGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCa          <  1:5087836/71‑1 (MQ=255)
                                                          cgcAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGAc       >  1:3192855/1‑71 (MQ=255)
                                                            cAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACgg     >  1:1891434/1‑71 (MQ=255)
                                                            cAAATTATTATCCTGACCGTATCCGATGCCTACAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACgg     >  1:4358/1‑71 (MQ=255)
                                                               attattATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTa  >  1:2826561/1‑71 (MQ=255)
                                                                ttattaTCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATa                      >  1:4446710/1‑50 (MQ=255)
                                                                ttattaTCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATa                      >  1:723691/1‑50 (MQ=255)
                                                                ttattaTCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATa                      >  1:2421513/1‑50 (MQ=255)
                                                                ttattaTCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATa                      >  1:2652166/1‑50 (MQ=255)
                                                                ttattaTCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATa                      >  1:4737424/1‑50 (MQ=255)
                                                                ttattaTCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATa                      >  1:4452882/1‑50 (MQ=255)
                                                                ttattaTCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTa  <  1:3479214/70‑1 (MQ=255)
                                                                ttattaTCCTGACCTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATa                      >  1:1541753/1‑49 (MQ=39)
                                                                      |                                                               
ATGAAAGGTATGAGTGGCCTGGATACTCTCAATGCCTTGCGCAGGGATGGCGTTACCGCGCAAATTATTATCCTGACCGTATCCGATGCCTCCAGCGATGTCTTTGCGCTGATAGACGCAGGCGCAGACGGTTA  >  Exported/1418235‑1418368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: