| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,684,513 | G→A | 100% | pseudogene (546/582 nt) pseudogene (1609/1645 nt) |
ilvG ← ilvG ← |
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,684,513 | 0 | G | A | 99.3% | 198.7 / ‑6.5 | 68 | pseudogene (546/582 nt) pseudogene (1609/1645 nt) | ilvG ilvG | ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (28/39); minor base C (1/0); total (29/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCGCCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGCCC > AP009048/3684448‑3684578 | gCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAgg > 1:3940929/1‑71 (MQ=255) cGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAggc > 1:3725122/1‑71 (MQ=255) ccGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCa > 1:503306/1‑71 (MQ=255) gATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCAcc < 1:3530105/71‑1 (MQ=255) aTACATTGACCTGATGTTACATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCAc > 1:2663593/1‑69 (MQ=255) tACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAggc < 1:397528/63‑1 (MQ=255) tACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAg > 1:3153083/1‑71 (MQ=255) aCATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGc > 1:2071776/1‑71 (MQ=255) cATTGACCTGATGTTGCATCATGATTATTTCTCCAACATTTCTGAATTACTGGCACCAggcgg > 1:4296380/1‑63 (MQ=255) cATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCg < 1:486312/71‑1 (MQ=255) cATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCg < 1:2306556/71‑1 (MQ=255) aTTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCa > 1:406965/1‑67 (MQ=255) aTTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGG‑GGCACCAGCGGc < 1:1052715/71‑1 (MQ=255) ttGACCTGATTTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCAcc < 1:3535191/65‑1 (MQ=255) ttGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCgg > 1:168978/1‑70 (MQ=255) tGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCCCCAGGCGGCACCAGCGGc > 1:3889269/1‑70 (MQ=255) tGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGc > 1:454415/1‑70 (MQ=255) gACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGcc < 1:2064550/70‑1 (MQ=255) aCCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCa < 1:4599936/70‑1 (MQ=255) ccTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGa < 1:3009696/71‑1 (MQ=255) cTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGAc > 1:3536809/1‑71 (MQ=255) tGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAggcggc < 1:2049132/56‑1 (MQ=255) gATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGt > 1:895455/1‑71 (MQ=255) aTGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGtt > 1:3687407/1‑71 (MQ=255) aTGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGtt > 1:4327682/1‑71 (MQ=255) gTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCAc < 1:1157732/54‑1 (MQ=255) gTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCAc < 1:911722/54‑1 (MQ=255) ttGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTtctc < 1:1375587/71‑1 (MQ=255) gCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCa < 1:2312292/70‑1 (MQ=255) atcatGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGt < 1:2974748/71‑1 (MQ=255) tcatGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGtt > 1:3144296/1‑71 (MQ=255) tcatGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGt < 1:2670476/70‑1 (MQ=255) catGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtc > 1:2985312/1‑71 (MQ=255) catGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtc > 1:27671/1‑71 (MQ=255) atGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCaa > 1:3175509/1‑66 (MQ=255) atGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCaa > 1:861833/1‑66 (MQ=255) atGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcg < 1:4486502/71‑1 (MQ=255) atGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtc < 1:1567635/70‑1 (MQ=255) tGATAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcg < 1:2642739/70‑1 (MQ=255) aTAATTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGc > 1:4113685/1‑50 (MQ=255) aaTTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAt > 1:743480/1‑71 (MQ=255) aTTTCTCCAACATTTCTGAATTACTGGCACCAGGCGGCAc < 1:655666/40‑1 (MQ=255) tttCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTg < 1:4374085/71‑1 (MQ=255) ttCTCCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt > 1:3197597/1‑69 (MQ=255) ctcCAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTgag < 1:4046541/70‑1 (MQ=255) ccAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGAtt < 1:570098/65‑1 (MQ=255) cAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTgaga < 1:1142373/68‑1 (MQ=255) cAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACa < 1:4173721/70‑1 (MQ=255) cAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACa < 1:2572313/70‑1 (MQ=255) cAACATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACAt > 1:4230708/1‑71 (MQ=255) aaCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTtc < 1:3516503/48‑1 (MQ=255) aaCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACa < 1:24356/69‑1 (MQ=255) aaCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACAt > 1:1473597/1‑70 (MQ=255) aaCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATg < 1:2466451/71‑1 (MQ=255) aCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtcgt < 1:2543916/57‑1 (MQ=255) aCATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGa < 1:3296020/71‑1 (MQ=255) cATTTCTGAATTACTGGCACCAGGCGGCACCAGCg > 1:2177453/1‑35 (MQ=255) cATTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGa > 1:3858242/1‑41 (MQ=255) aTTTCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAg < 1:2187489/71‑1 (MQ=255) tttCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGc < 1:1797203/71‑1 (MQ=255) tttCTGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGc < 1:2601828/71‑1 (MQ=255) tGAATTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTtc > 1:3700283/1‑48 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAgg < 1:2869810/68‑1 (MQ=255) aaTTACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGt < 1:3974242/69‑1 (MQ=255) ttACTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTgaga > 1:4391159/1‑55 (MQ=255) cTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGccc < 1:2474276/71‑1 (MQ=255) cTGGCACCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGcc < 1:3662921/70‑1 (MQ=255) tGGCACCAGGCGGCACCAGCGGCCAGACGTTGTCAAGTTCGTCGAt < 1:3958218/46‑1 (MQ=39) | GCGAGCCGATACATTGACCTGATGTTGCATCATGATAATTTCTCCAACATTTCTGAATTACTGGCGCCAGGCGGCACCAGCGGCCAGACGTTCTCAAGTTCGTCGATTGAGACATGAAGCAGGTATGGCCC > AP009048/3684448‑3684578 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |