| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,684,870 | C→T | 100% | pseudogene (189/582 nt) pseudogene (1252/1645 nt) |
ilvG ← ilvG ← |
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,684,870 | 0 | C | T | 100.0% | 84.4 / NA | 29 | pseudogene (189/582 nt) pseudogene (1252/1645 nt) | ilvG ilvG | ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/13); total (16/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGGTGCCCAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAG > AP009048/3684803‑3684936 | cGGTGCCCAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGt < 1:2293178/71‑1 (MQ=255) gTGCCCAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTcg < 1:3668378/71‑1 (MQ=255) gTGCCCAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTcg < 1:2689789/71‑1 (MQ=255) cccAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTcgcgc > 1:1823669/1‑71 (MQ=255) cccAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTcgcg > 1:2187341/1‑70 (MQ=255) ccAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTcgcgcg < 1:3900021/71‑1 (MQ=255) ctctTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACtt > 1:4015299/1‑71 (MQ=255) caTTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCTTTTGGTCGCGCGACTTgcgcgc > 1:844571/1‑69 (MQ=255) caTTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGAc > 1:4303600/1‑61 (MQ=255) aTTCATCATGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCaa > 1:3198104/1‑71 (MQ=255) tcatcaTGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAc > 1:1155633/1‑70 (MQ=255) catcatGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAccg > 1:1169194/1‑71 (MQ=255) tcatGAAAGAGCCGTCACCGGAGATACACACGACGGTATCGTTTGGTCGCGCGACTtgcg < 1:3098278/60‑3 (MQ=255) atGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTcg > 1:1198704/1‑47 (MQ=39) atGAAAGAGCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAccgc > 1:2810192/1‑68 (MQ=255) gCCGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAAc < 1:345486/57‑1 (MQ=255) ccGTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTgcgc > 1:2526026/1‑50 (MQ=255) gTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCa > 1:2767012/1‑70 (MQ=255) gTCACCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCa > 1:2807933/1‑70 (MQ=255) aCCGGAGATACAGACGACGGTATCGTTTGGTCGCGCGACtt > 1:38165/1‑41 (MQ=38) gaTACAGACGACGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATgg < 1:1477288/71‑1 (MQ=39) cAGACGACGGTATCGTTTGGTCGCGCGACGTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGcc < 1:1743894/71‑1 (MQ=37) gacgacGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTa < 1:2187360/71‑1 (MQ=38) gacgacGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTa < 1:3049590/71‑1 (MQ=38) cgaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTaag > 1:1740978/3‑70 (MQ=38) cgaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTaa < 1:2470319/68‑1 (MQ=38) gaCGGTATCGTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAAcc < 1:1986778/50‑1 (MQ=255) gTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTg > 1:4053355/1‑67 (MQ=37) gTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAg < 1:297134/70‑1 (MQ=38) | CGGTGCCCAGCTCTTGCACATTCATCATGAAAGAGCCGTCACCGGAGATACAGACAACGGTATCGTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAG > AP009048/3684803‑3684936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |