| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,735,427 | G→A | 13.0% | A323T (GCA→ACA) | bglH → | carbohydrate‑specific outer membrane porin, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,735,427 | 0 | G | A | 13.0% | 193.7 / 16.2 | 69 | A323T (GCA→ACA) | bglH | carbohydrate‑specific outer membrane porin, cryptic |
| Reads supporting (aligned to +/- strand): ref base G (40/20); new base A (3/6); total (43/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.18e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
AGATATCCCGTTATGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGTTTGGCACAT > AP009048/3735360‑3735496 | aGATATCCCGTTATGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCa > 1:1241909/1‑70 (MQ=255)aGATATCCCGTTATGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCAt > 1:1577846/1‑71 (MQ=255) cccGTTATGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGaa > 1:110677/1‑70 (MQ=255) tGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATa < 1:437396/70‑1 (MQ=255) ggATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCg < 1:4240830/58‑1 (MQ=255) ggATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAAt < 1:1986990/70‑1 (MQ=255) gATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCa < 1:3236721/71‑1 (MQ=255) gATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAAt > 1:3647970/1‑69 (MQ=255) aaaGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGa > 1:613475/1‑71 (MQ=255) aaaGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGa > 1:762663/1‑71 (MQ=255) aGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATc > 1:3775903/1‑65 (MQ=255) gCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGataa > 1:63451/1‑71 (MQ=255) ccACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGataat < 1:3723255/71‑1 (MQ=255) cACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGat > 1:2770049/1‑67 (MQ=255) cACCTTAATGGTAAGTGGTCGTTATGGCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGat > 1:3943907/1‑67 (MQ=255) ccTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAAc > 1:3061599/1‑71 (MQ=255) cTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGAtaataa > 1:4058196/1‑69 (MQ=255) aaTGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACggg < 1:4154125/70‑1 (MQ=255) gTAAGTGGTCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGtatta < 1:1524966/71‑1 (MQ=255) tAAGTGGTCGTTATGTCCCGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGtatt < 1:3759706/69‑1 (MQ=255) tAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGtattat < 1:3754481/71‑1 (MQ=255) gTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACggg > 1:949483/1‑62 (MQ=255) gTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGTGTATTATGAc < 1:1086422/71‑1 (MQ=255) gTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGt > 1:1070740/1‑63 (MQ=255) gTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGAc < 1:3307508/71‑1 (MQ=255) tGGTCGATATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATaa > 1:4575297/1‑45 (MQ=255) gttCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGata > 1:2534799/3‑52 (MQ=255) ggTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATaa > 1:1168889/1‑44 (MQ=255) gTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTg < 1:2720148/70‑1 (MQ=255) gTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTg < 1:1310850/70‑1 (MQ=255) tCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACggg < 1:397965/58‑1 (MQ=255) tCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGa < 1:311577/71‑1 (MQ=255) tCGTTATGTCACGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATg < 1:1603127/65‑1 (MQ=255) cGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAgg > 1:4347890/1‑46 (MQ=255) cGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGa > 1:838588/1‑70 (MQ=255) gTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCATGATAATAACGGGTATTATGACTGGaaa < 1:832800/71‑1 (MQ=255) gTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACggg < 1:118057/56‑1 (MQ=255) gTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGa > 1:4252503/1‑69 (MQ=255) gTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGa > 1:3252735/1‑69 (MQ=255) ttttGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGtattat < 1:3295593/58‑1 (MQ=255) tATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGaaa > 1:2186976/1‑69 (MQ=255) tATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGa > 1:3379548/1‑67 (MQ=255) aTGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGataat > 1:2465450/1‑47 (MQ=255) gTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACggg < 1:2282640/51‑1 (MQ=255) gTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATAc > 1:694248/1‑71 (MQ=255) cACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAgg > 1:2944018/1‑38 (MQ=255) aCGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAAc > 1:1726383/1‑45 (MQ=255) aCGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAAc > 1:2986769/1‑45 (MQ=255) aCGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTg > 1:4567935/1‑71 (MQ=255) aCGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTg < 1:430687/71‑1 (MQ=255) aCGGCAAACGAAAGCACATCGGAAAAAGATAATCAGGATAATAGCGGGTATTATGACt < 1:3965932/58‑1 (MQ=255) cGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGtatta > 1:1931776/1‑52 (MQ=255) cGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTg > 1:3570816/1‑70 (MQ=255) cGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTg > 1:651367/1‑70 (MQ=255) cAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTgg > 1:2771534/1‑56 (MQ=255) cAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATg > 1:3605967/1‑71 (MQ=255) aaaCGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGt > 1:981888/1‑71 (MQ=255) aaaCGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGt < 1:4520330/71‑1 (MQ=255) aaCGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACggg < 1:4158729/42‑1 (MQ=255) aaCGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGt > 1:572110/1‑43 (MQ=255) aaCGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGt > 1:2159920/1‑43 (MQ=255) aaCGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAg > 1:2002108/1‑58 (MQ=255) aCGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGttt > 1:2567570/1‑71 (MQ=255) aCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACt > 1:2828654/1‑51 (MQ=255) aCGAAAGCACATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGttt < 1:94274/71‑1 (MQ=255) cGAAAGCACATCGGAAAAAGATAATCAGGAtaataa > 1:3307212/1‑36 (MQ=255) gAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGa < 1:564757/52‑1 (MQ=255) aaGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGTTTGGca > 1:685449/1‑71 (MQ=255) aGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGTTTGGca > 1:2284043/1‑70 (MQ=255) gcgcATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGTTTGGcaca < 1:2172731/71‑1 (MQ=255) cgcATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGTTTGGc > 1:4276291/1‑67 (MQ=255) gcATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGTTTGGCACAt < 1:2285607/70‑1 (MQ=255) | AGATATCCCGTTATGGGATAAAGCCACCTTAATGGTAAGTGGTCGTTATGTCACGGCAAACGAAAGCGCATCGGAAAAAGATAATCAGGATAATAACGGGTATTATGACTGGAAAGATACCTGGATGTTTGGCACAT > AP009048/3735360‑3735496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |