| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,778,494 | C→T | 8.2% | pseudogene (67/639 nt) | glvG → | ECK3673:JW3658:b3681; predicted 6‑phospho‑beta‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,778,494 | 0 | C | T | 8.2% | 191.0 / 4.8 | 61 | pseudogene (67/639 nt) | glvG | ECK3673:JW3658:b3681; predicted 6‑phospho‑beta‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base C (25/31); new base T (3/2); total (28/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGACCAAATTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGATGGTGCGCGGC > AP009048/3778427‑3778560 | aaTGACCAAATTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTcc > 1:1898692/1‑71 (MQ=255) aTGACCAAATTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCt < 1:2736713/71‑1 (MQ=255) aCCAAATTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGc > 1:309695/1‑71 (MQ=255) cAAATTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTg < 1:3843516/67‑1 (MQ=255) cAAATTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGa < 1:1388653/71‑1 (MQ=255) aaaTTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGaa > 1:3870015/1‑71 (MQ=255) aaTTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGaa < 1:527602/70‑1 (MQ=255) aaTTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAAt > 1:1408686/1‑71 (MQ=255) aaTTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTACTGGCGAAt > 1:455130/1‑71 (MQ=255) aTTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATc < 1:1266428/71‑1 (MQ=255) aTTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATc < 1:3561705/71‑1 (MQ=255) ctcAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAg < 1:658843/70‑1 (MQ=255) ctcAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAg < 1:201951/70‑1 (MQ=255) aGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGa < 1:2558937/69‑1 (MQ=255) gTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCg < 1:1724931/71‑1 (MQ=255) tGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGt < 1:2653779/71‑1 (MQ=255) tGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGt > 1:2516170/1‑71 (MQ=255) gTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGaa < 1:4154274/59‑1 (MQ=255) gTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGtt < 1:1727721/70‑1 (MQ=255) gTTGTCGCAGGCGGGGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGttt < 1:2156077/71‑1 (MQ=255) tGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTcc > 1:3544446/1‑71 (MQ=255) gTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTcc > 1:3129210/1‑70 (MQ=255) tCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTcc < 1:545278/48‑1 (MQ=255) cGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAgg > 1:1596985/1‑59 (MQ=255) gCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCt < 1:1310193/71‑1 (MQ=255) aGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTc < 1:2140554/71‑1 (MQ=255) gCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGt > 1:1211236/1‑71 (MQ=255) ggTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGtt > 1:1105490/1‑58 (MQ=255) ggAAGCACCTTTACGCCAGGCATCGTGTTGATGCTcc < 1:2058395/37‑1 (MQ=255) ggAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGAc < 1:4479620/51‑1 (MQ=255) aaGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCa < 1:2004886/67‑1 (MQ=255) aGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGa > 1:2914553/1‑70 (MQ=255) aCCTTTACGCCAGGCATCGTGTTGATGTTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTg < 1:3323331/66‑1 (MQ=255) aCCTTTACGCCAGGCATCGTGTTGATGTTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAAtt < 1:298745/71‑1 (MQ=255) ccTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAAttt < 1:3965243/71‑1 (MQ=255) cTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAAttt > 1:2220093/1‑70 (MQ=255) tttACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGt < 1:3642453/45‑1 (MQ=255) ttACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTa > 1:1684361/1‑70 (MQ=255) tACGCCAGGCATCGTGTTGATGTTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTAt > 1:2612496/1‑70 (MQ=255) tACGCCAGGCATCGTGTTGATGTTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTAt > 1:1684566/1‑70 (MQ=255) tACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTa < 1:475719/69‑1 (MQ=255) tACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTAt > 1:3709366/1‑70 (MQ=255) aCGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATg < 1:2766333/70‑1 (MQ=255) cAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCtt > 1:2817744/1‑47 (MQ=255) cAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAAc < 1:497414/71‑1 (MQ=255) aGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCg > 1:3385392/1‑48 (MQ=255) ggCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGa < 1:1939853/71‑1 (MQ=255) ggCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGa > 1:1483549/1‑71 (MQ=255) ggCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGa > 1:2798170/1‑71 (MQ=255) gCATCGTGTTGATGCTCCTGGCGATTCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGAt < 1:4110421/71‑1 (MQ=255) gCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGAt > 1:1595087/1‑71 (MQ=255) tCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTg < 1:1581328/45‑1 (MQ=255) tCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGATGGt > 1:1656647/1‑71 (MQ=255) gtgtTGATGCTCCTGGCGAATCAGGAGCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGATGGTg < 1:2463343/70‑1 (MQ=255) gtgtTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTa > 1:3379259/1‑56 (MQ=255) ttGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGATGGTgcgc < 1:4470799/70‑1 (MQ=255) tGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGATgg > 1:2154295/1‑64 (MQ=255) tGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGATGGTGcgcg > 1:1835135/1‑70 (MQ=255) gATGTTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGATGGTGCGCgg > 1:3651446/1‑70 (MQ=255) gATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGATGGTGCGCgg > 1:382990/1‑70 (MQ=255) aTGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGATGGTGCGCGGc < 1:3643251/70‑1 (MQ=255) | AATGACCAAATTCTCAGTGGTTGTCGCAGGCGGTGGAAGCACCTTTACGCCAGGCATCGTGTTGATGCTCCTGGCGAATCAGGACCGTTTCCCGCTTCGTGCACTGAAATTTTATGATAACGATGGTGCGCGGC > AP009048/3778427‑3778560 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |