| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,806,471 | G→A | 13.6% | P198P (CCG→CCA) | yicI → | predicted alpha‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,806,471 | 0 | G | A | 13.6% | 181.0 / 17.1 | 66 | P198P (CCG→CCA) | yicI | predicted alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base G (28/29); new base A (3/6); total (31/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
GGCCAGACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAGTGG > AP009048/3806403‑3806538 | ggCCAGACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGt > 1:3409070/1‑70 (MQ=255) ccAGACGGTAGAGACCTGGAACCGGTACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCt < 1:2820921/71‑1 (MQ=255) ccAGACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCt < 1:1423655/71‑1 (MQ=255) aGACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTa > 1:4000463/1‑70 (MQ=255) aGACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTa > 1:2923996/1‑70 (MQ=255) aCGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACAt < 1:917456/71‑1 (MQ=255) cGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACAt < 1:2569680/70‑1 (MQ=255) tAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACt < 1:1307357/71‑1 (MQ=255) tAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACt < 1:2949057/71‑1 (MQ=255) gaCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAAcc > 1:2488032/1‑71 (MQ=255) aCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAAc < 1:2057281/69‑1 (MQ=255) aCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCg > 1:970264/1‑71 (MQ=255) aCCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCATTCTACATGACTAAcc < 1:4062959/70‑1 (MQ=255) cTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTAc < 1:4291075/58‑1 (MQ=255) ggAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGt > 1:1864433/1‑71 (MQ=255) ggAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGt > 1:2094534/1‑71 (MQ=255) aCCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTAt < 1:3428702/71‑1 (MQ=255) ccGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATg > 1:361555/1‑71 (MQ=255) ccGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATg > 1:4252650/1‑71 (MQ=255) gggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACt > 1:279977/1‑56 (MQ=255) ggACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGt > 1:1366144/1‑64 (MQ=255) gcggcACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACa > 1:1541747/1‑45 (MQ=255) cggcACAAGTACTGAAGAGGCGTATAAAAATATCCCGTTCTACATGACTAAcc < 1:1671158/53‑1 (MQ=255) cggcACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCt < 1:2490273/41‑1 (MQ=255) gcACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTc < 1:1519970/71‑1 (MQ=255) gcACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTc < 1:1117383/71‑1 (MQ=255) cacaAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAAc < 1:722912/49‑1 (MQ=255) cacaAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCa < 1:4395032/71‑1 (MQ=255) acaAGTACTGAACAGTCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATg < 1:4146706/58‑1 (MQ=255) acaAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTgg < 1:2639386/53‑1 (MQ=255) acaAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCaa > 1:366284/1‑71 (MQ=255) agcaCTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatca > 1:3256648/4‑71 (MQ=255) aGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGtt < 1:4502033/52‑1 (MQ=255) gTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTAt < 1:1409099/53‑1 (MQ=255) tACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTa < 1:2340589/51‑1 (MQ=255) tGAACAGGCGTATAAAAATATCCCGTTCTACATGACTa < 1:1965907/38‑1 (MQ=255) gAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATccc > 1:1232308/1‑69 (MQ=255) aaCAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATgggg > 1:2959666/1‑51 (MQ=255) aaCAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATgggg > 1:715237/1‑51 (MQ=255) aaCAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAg > 1:1723469/1‑71 (MQ=255) aaCAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAg < 1:1156700/71‑1 (MQ=255) aaCAGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAg > 1:3036984/1‑71 (MQ=255) cAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTAc > 1:928055/1‑52 (MQ=255) cAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTAc > 1:1331393/1‑52 (MQ=255) aGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAgtgt > 1:1598920/1‑71 (MQ=255) aGGCGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAgtgt < 1:977145/71‑1 (MQ=255) cGTATAAAAATATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGtc < 1:817637/71‑1 (MQ=255) tataAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGtctc < 1:4207596/71‑1 (MQ=255) tataAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGtctc < 1:3308065/71‑1 (MQ=255) taAAAATATCCCGTTCTACATGACTAACCGTGGTTa < 1:3424266/36‑1 (MQ=255) taAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTctct < 1:4214598/70‑1 (MQ=255) taAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCtt > 1:3584495/1‑71 (MQ=255) taAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCtt > 1:3988732/1‑71 (MQ=255) aaaaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATcccc < 1:4223166/58‑1 (MQ=255) aaaaTATCCCGTTCTACATGACTAACCGTGGTTATgg > 1:315136/1‑37 (MQ=255) aaaaTATCCCGTTCTACATGACTAACCGTGGTTATg > 1:1733543/1‑36 (MQ=255) aaaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCtttt < 1:3932069/71‑1 (MQ=255) aaaaTATCCCATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCtttt > 1:834914/1‑71 (MQ=255) aaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAatcatc > 1:2910050/1‑53 (MQ=255) aaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAgtgt < 1:1159404/61‑1 (MQ=255) aaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCtttt > 1:2079431/1‑70 (MQ=255) aaaTATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTg < 1:1368113/71‑1 (MQ=255) atatCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTg > 1:3014332/1‑69 (MQ=255) tCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAGt < 1:502574/70‑1 (MQ=255) cccATTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAGTgg > 1:69136/1‑71 (MQ=255) cGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCttt < 1:2202604/61‑1 (MQ=255) | GGCCAGACGGTAGAGACCTGGAACCGGGACGGCGGCACAAGTACTGAACAGGCGTATAAAAATATCCCGTTCTACATGACTAACCGTGGTTATGGGGTACTGGTCAATCATCCCCAGTGTGTCTCTTTTGAAGTGG > AP009048/3806403‑3806538 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |