| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,051 | G→A | 100% | E135E (GAG→GAA) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,051 | 0 | G | A | 100.0% | 249.7 / NA | 81 | E135E (GAG→GAA) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/47); total (34/47) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTC > AP009048/3824983‑3825115 | ggtAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATc > 1:2165954/1‑71 (MQ=255) gtAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATc < 1:2494876/70‑1 (MQ=255) gtAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCg < 1:4512522/71‑1 (MQ=255) tAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGa < 1:445446/71‑1 (MQ=255) tAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGa > 1:984052/1‑71 (MQ=255) gcgcGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAt < 1:2491985/70‑1 (MQ=255) gcgcGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGAt > 1:4236615/1‑70 (MQ=255) gcgcGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg < 1:2626659/71‑1 (MQ=255) cgTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATg > 1:2295082/1‑68 (MQ=255) cgTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGgag < 1:632462/71‑1 (MQ=255) aaGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattatt > 1:151699/1‑71 (MQ=255) aGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattatt < 1:525086/70‑1 (MQ=255) aGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattatt < 1:4097897/70‑1 (MQ=255) aGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTc < 1:1325170/71‑1 (MQ=255) ggACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattatt < 1:771205/69‑1 (MQ=255) cgcgTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAgg < 1:2115368/70‑1 (MQ=255) gTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGct > 1:1640609/1‑68 (MQ=255) gTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:451702/1‑71 (MQ=255) gTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa < 1:291911/71‑1 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGct < 1:81738/68‑2 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa < 1:2912019/70‑1 (MQ=255) aaTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg > 1:406701/1‑71 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGgaga < 1:4013524/55‑1 (MQ=255) tGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATc < 1:4019314/46‑1 (MQ=255) tGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATc < 1:3280093/46‑1 (MQ=255) tGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgc < 1:1275995/71‑1 (MQ=255) gCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattat < 1:3334781/57‑1 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattat < 1:1166505/56‑1 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGatta < 1:1052266/55‑1 (MQ=255) ggTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAg < 1:4355147/58‑1 (MQ=255) gTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGAc < 1:3705377/71‑1 (MQ=255) gTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGAc > 1:2804411/1‑71 (MQ=255) tAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGc > 1:4231823/1‑58 (MQ=255) tAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGAc > 1:1504258/1‑70 (MQ=255) gatgatGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattatt < 1:4116019/51‑1 (MQ=255) tgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGcgt > 1:1320742/1‑68 (MQ=255) tgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTg < 1:549396/71‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGgag < 1:1147421/40‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGcgcg < 1:2105981/61‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg > 1:3300849/1‑71 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg < 1:2417389/71‑1 (MQ=255) gtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTg < 1:885950/67‑1 (MQ=255) tgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGattatt < 1:637518/44‑1 (MQ=255) tgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa < 1:3738126/52‑1 (MQ=39) tgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGATGGc > 1:4149700/1‑71 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGatta > 1:3489574/1‑39 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgta > 1:1649644/1‑70 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGATGGCgta < 1:1124544/71‑2 (MQ=255) cACCGCCGGAACGGCGATTCGCTAATCGATGGAGattatt < 1:3819686/40‑1 (MQ=38) cACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgtat > 1:1586172/1‑69 (MQ=255) aCCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTAtt > 1:3431158/1‑71 (MQ=255) ccgccgGAACGGCGATTCGCGAATCGATGGAGatta < 1:2559948/36‑1 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGa > 1:212467/1‑71 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGCCGCTTGCTgg > 1:2632190/1‑61 (MQ=255) ggAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTc < 1:2888825/71‑1 (MQ=255) gAACGGCGATTCGCGAATCGATGGAGATTATTCAGGct < 1:876001/38‑2 (MQ=38) aaCGGCGATTCGCGAATCGATGGAGATTATTCAGGct > 1:3459322/1‑36 (MQ=38) aaCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:1641416/71‑1 (MQ=255) aaCGGCGATTCGCGAATCGATGGAGATTATTCAGGATAATGGCGCGa > 1:2647512/1‑47 (MQ=37) aCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCt > 1:1367202/1‑54 (MQ=39) aCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCt > 1:3202809/1‑71 (MQ=255) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt < 1:4295501/71‑2 (MQ=255) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt > 1:1341393/1‑70 (MQ=255) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt < 1:4522355/71‑2 (MQ=255) ggCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:162912/68‑1 (MQ=255) ggCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt > 1:1754405/1‑69 (MQ=255) gCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCt > 1:646567/1‑68 (MQ=255) gCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGa > 1:2788483/1‑71 (MQ=255) gCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGa > 1:3304369/1‑71 (MQ=255) cGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg < 1:1714816/37‑1 (MQ=37) cGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCt > 1:3030462/1‑67 (MQ=255) cGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGa > 1:4403973/1‑70 (MQ=255) gATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATc > 1:2647793/1‑71 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTg < 1:1324806/45‑1 (MQ=38) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:517736/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:2687081/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:3742535/71‑1 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt > 1:4349746/1‑70 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTc > 1:866565/1‑59 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCg < 1:1977238/68‑1 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTc < 1:2946924/70‑1 (MQ=255) | GGTAGCGCGTTACAAGGACGCGTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTC > AP009048/3824983‑3825115 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |