| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,213 | T→C | 100% | A189A (GCT→GCC) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,213 | 0 | T | C | 100.0% | 215.3 / NA | 63 | A189A (GCT→GCC) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/35); total (28/35) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCGCGAAGAG > AP009048/3825145‑3825276 | aTTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTa > 1:1231590/1‑71 (MQ=255) aGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTg < 1:156869/71‑1 (MQ=255) ggAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTatc < 1:1919417/68‑3 (MQ=255) ggAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTat < 1:3574004/67‑2 (MQ=255) ggAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTg > 1:1148170/1‑70 (MQ=255) gAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTgg > 1:4062535/1‑70 (MQ=255) aGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAag < 1:2579895/71‑1 (MQ=255) gTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAATATCTGATTGCCTATCTGGAaga < 1:3929/71‑1 (MQ=255) ttGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag < 1:437638/71‑1 (MQ=255) ttGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag < 1:3682894/71‑1 (MQ=255) tGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag < 1:645461/70‑1 (MQ=255) tGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag > 1:4369589/1‑70 (MQ=255) gAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag > 1:4090439/1‑69 (MQ=255) aGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAg < 1:1848380/71‑1 (MQ=255) gCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGc < 1:3587294/71‑1 (MQ=255) gATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAag < 1:2919795/61‑1 (MQ=255) aTTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGa < 1:1243729/70‑1 (MQ=255) aTTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGa < 1:3154490/70‑1 (MQ=255) tACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGaaa > 1:4256143/1‑70 (MQ=255) tACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAAt > 1:3122057/1‑71 (MQ=255) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATgg < 1:1157502/71‑1 (MQ=255) aaCTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGc < 1:1574414/71‑1 (MQ=255) cTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCgg < 1:892133/71‑1 (MQ=255) cTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCg > 1:4268880/1‑70 (MQ=255) cTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCg > 1:3141773/1‑70 (MQ=255) cAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGaa < 1:1534286/70‑1 (MQ=255) cAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGa < 1:3117652/69‑1 (MQ=255) aaGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAaga > 1:4512178/1‑49 (MQ=37) gTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCt < 1:509692/71‑1 (MQ=255) tGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATgg < 1:2658754/60‑1 (MQ=255) aTCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGAGGAACATCTgg > 1:2263009/1‑70 (MQ=255) ctctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTggcgg > 1:3141809/1‑71 (MQ=255) tctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGc < 1:3654010/56‑1 (MQ=38) tctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCgg < 1:2098638/58‑1 (MQ=38) ctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAaga < 1:3547468/40‑1 (MQ=25) ctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATc > 1:3235700/1‑63 (MQ=255) ctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATc > 1:3254212/1‑63 (MQ=255) ctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCt > 1:3900517/1‑64 (MQ=255) ctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTg > 1:408241/1‑65 (MQ=255) tcatcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt < 1:2691774/71‑1 (MQ=255) tcatcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt < 1:2049608/71‑1 (MQ=255) catcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt > 1:2346770/1‑70 (MQ=255) catcaACCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt > 1:2658198/1‑70 (MQ=255) atcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGAGGCGGTTa < 1:1229661/70‑1 (MQ=255) tcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGaaa < 1:808548/45‑1 (MQ=37) tcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCgg < 1:1457950/51‑1 (MQ=38) tcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAg > 1:115142/1‑71 (MQ=255) aCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTggcg > 1:1805099/1‑60 (MQ=255) cccTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGcc > 1:1544886/1‑71 (MQ=255) cTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAg < 1:831298/66‑1 (MQ=39) gAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGa < 1:3767437/36‑1 (MQ=21) aaaGATCTGATTGCCTATCTGGAAGAGAAGCCGGaaa > 1:4346466/1‑37 (MQ=21) aGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTaa > 1:3013971/1‑60 (MQ=38) gatcTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATcgcg < 1:1406325/67‑1 (MQ=255) atcTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt < 1:825021/54‑1 (MQ=38) cTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCt < 1:3054520/44‑1 (MQ=38) tGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACAt > 1:3984845/1‑41 (MQ=38) tGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt < 1:2935577/53‑1 (MQ=38) gATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGaa > 1:2602652/1‑37 (MQ=37) gATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGaa > 1:488464/1‑37 (MQ=37) gATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGaa > 1:842866/1‑37 (MQ=37) aTTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGAGGCGGTTAAGGCCTATCTCGAagag < 1:22680/69‑1 (MQ=255) tGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTgg > 1:2449540/1‑41 (MQ=37) | ATTCAGGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCGCGAAGAG > AP009048/3825145‑3825276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |