| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 4,628,826 | C→T | 8.4% | *215* (TGA→TAA) | ytjB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 4,628,826 | 0 | C | T | 8.4% | 182.0 / 5.3 | 59 | *215* (TGA→TAA) | ytjB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (23/31); new base T (2/3); total (25/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
CGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCT > AP009048/4628761‑4628895 | tggaaGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActct < 1:1323987/68‑1 (MQ=255) gTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActct < 1:3602343/70‑1 (MQ=255) gTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActct > 1:2115380/1‑70 (MQ=255) aaGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTTACTCtttt > 1:1061739/1‑71 (MQ=255) aaGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCtttt > 1:2254113/1‑71 (MQ=255) agagTCAGAGATGAGCTGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCttttt < 1:2234133/71‑1 (MQ=255) agagTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTACTGGGTTACTCttttt > 1:2666783/1‑71 (MQ=255) agagTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCttttt < 1:3438335/71‑1 (MQ=255) agagTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCttttt < 1:1885416/71‑1 (MQ=255) gagTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCttttt > 1:3498373/1‑70 (MQ=255) gTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtc < 1:109322/70‑1 (MQ=255) gTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtc > 1:4573244/1‑70 (MQ=255) tCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTc < 1:4319793/58‑1 (MQ=255) agaTGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTTACTCTTTTTTCTCGCtt < 1:2579012/71‑1 (MQ=255) agaTGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCtt > 1:2286332/1‑71 (MQ=255) gaTGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCttt > 1:3268295/1‑71 (MQ=255) gaTGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCtt < 1:1702333/70‑1 (MQ=255) aTGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTTACTCTTTTTTCTCGCttt < 1:1463777/70‑1 (MQ=255) aGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcctc > 1:1353986/1‑71 (MQ=255) tAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCa < 1:2312151/42‑1 (MQ=255) tAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCg < 1:2435347/71‑1 (MQ=255) tAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCg > 1:2405887/1‑71 (MQ=255) aTGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGc < 1:1611602/71‑1 (MQ=255) tgGACATAACGATTTCCTTTCTTGTAGTAATGGGTTACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCAc < 1:901840/70‑1 (MQ=255) ggACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCAc < 1:4083031/69‑1 (MQ=255) aaCGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGttt > 1:1074513/1‑69 (MQ=255) aaCGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTg > 1:14258/1‑70 (MQ=255) aaCGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGc > 1:2302650/1‑71 (MQ=255) aaCGATTTCCTTTCTTGTAGTAATGGGGCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGtt < 1:1287529/68‑1 (MQ=255) aCGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTg > 1:1492215/1‑69 (MQ=255) cGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtc > 1:4391227/1‑38 (MQ=255) tttCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCa > 1:1850471/1‑57 (MQ=255) ttCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCgg > 1:1974938/1‑71 (MQ=255) ttCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCgg > 1:3725544/1‑71 (MQ=255) tCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTtcttcct > 1:2629472/1‑46 (MQ=255) tCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCgg < 1:164324/70‑1 (MQ=255) cTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGtt < 1:2086382/70‑1 (MQ=255) tttCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTttcttc < 1:3030870/41‑1 (MQ=255) tttCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTttcttc < 1:624688/41‑1 (MQ=255) tttCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGttt > 1:4036221/1‑59 (MQ=255) ttCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTATTCCTCTTCCGGCACCGGTTTGCTGGCGGTTaa > 1:4300417/1‑70 (MQ=255) ctttagtaATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAg < 1:4376547/67‑1 (MQ=255) tGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAgg > 1:2647609/1‑70 (MQ=255) gtagtaATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGc > 1:4254644/1‑45 (MQ=255) gtagtaATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGa < 1:4011778/70‑1 (MQ=255) tagtaATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGaa < 1:4010166/70‑1 (MQ=255) gtaATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAggg < 1:3667081/71‑1 (MQ=255) gtaATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAgg < 1:4604924/70‑1 (MQ=255) taATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCAc < 1:4075858/43‑1 (MQ=255) taATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGc < 1:2810517/71‑1 (MQ=255) taATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGc < 1:2976132/71‑1 (MQ=255) taATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGc < 1:156571/71‑1 (MQ=255) aTGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTgg < 1:3639678/52‑1 (MQ=255) ggTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGtt > 1:1141158/1‑43 (MQ=255) ggTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATtg < 1:2518446/71‑1 (MQ=255) ggTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATtg > 1:3244787/1‑71 (MQ=255) gTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTa < 1:3775445/54‑1 (MQ=255) gTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATtgc < 1:1726146/71‑1 (MQ=255) tCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTaa < 1:2346432/54‑1 (MQ=255) tCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATtgct < 1:2816373/71‑1 (MQ=255) cACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATtgct < 1:1355246/70‑1 (MQ=255) | CGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCT > AP009048/4628761‑4628895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |