| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 459,715 | A→G | 16.2% | Y535C (TAC→TGC) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 459,715 | 0 | A | G | 16.2% | 164.4 / 23.9 | 68 | Y535C (TAC→TGC) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base A (20/37); new base G (4/7); total (24/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTT > AP009048/459645‑459779 | gAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTtacta > 1:3378057/1‑71 (MQ=255) cGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTacac < 1:2151903/71‑1 (MQ=255) tAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCAccc < 1:3543018/71‑1 (MQ=255) aTGCACTGAAAAAAGGTTAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGt < 1:2901884/71‑1 (MQ=255) aTGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGt < 1:3850853/71‑1 (MQ=255) tGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGt < 1:162622/70‑1 (MQ=255) cACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGt > 1:184060/1‑68 (MQ=255) cACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAg > 1:416768/1‑71 (MQ=255) cTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggc > 1:2232850/1‑71 (MQ=255) tGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggcg > 1:2995417/1‑71 (MQ=255) gAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAggcgg > 1:1181965/1‑71 (MQ=255) aaaaaaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGa > 1:1321866/1‑65 (MQ=255) aaaaaaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggcggg > 1:1257522/1‑71 (MQ=255) aaaaaaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggcgg > 1:230276/1‑70 (MQ=255) aaaaaaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggc < 1:1550027/68‑1 (MQ=255) aaaaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAg > 1:625953/1‑64 (MQ=255) aaaaGGTGAGCTGACCGTCGACGATAGCGCCATTATAGGCATTATTCGTTACTGCACCCGTGAggcgggcg > 1:3302761/1‑71 (MQ=255) aaaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgt < 1:2930484/71‑1 (MQ=255) aaaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgt < 1:2563264/71‑1 (MQ=255) aaGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgtg < 1:2696006/71‑1 (MQ=255) gTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgtgcg < 1:4091520/70‑1 (MQ=255) tGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgt < 1:512834/66‑1 (MQ=255) tGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGcgtgcgtg < 1:3558923/71‑1 (MQ=255) tGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGcgtgcgtg < 1:519567/71‑1 (MQ=255) tGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGcgtgcgtg < 1:951240/71‑1 (MQ=255) gAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGgcgtgcg > 1:2589852/1‑68 (MQ=255) aGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTgg < 1:915444/70‑1 (MQ=255) aGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTgg < 1:871607/70‑1 (MQ=255) tGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTc < 1:3867926/69‑1 (MQ=255) gACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGa > 1:2555711/1‑51 (MQ=255) aCCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTg < 1:1903336/69‑1 (MQ=255) aCCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTg < 1:231596/69‑1 (MQ=255) cGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGc > 1:2169902/1‑71 (MQ=255) tCGACGATAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGt < 1:3040441/71‑1 (MQ=255) tCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgt < 1:3665111/55‑1 (MQ=255) tCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgt < 1:3659833/55‑1 (MQ=255) tCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGt > 1:3181184/1‑71 (MQ=255) tCGACGATAGAGCCATTATCGGCATTATTCGTTACTACACCCGTGAggcgg < 1:4066487/51‑1 (MQ=255) cgacgaTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGt > 1:4164242/1‑70 (MQ=255) gacgaTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGt < 1:4086539/69‑1 (MQ=255) gacgaTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTg < 1:2021657/70‑1 (MQ=255) gacgaTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTg > 1:1573961/1‑70 (MQ=255) acgaTAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGa < 1:2224603/43‑1 (MQ=255) aTAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAggc < 1:3599815/43‑1 (MQ=255) aTAGCGCCATTATCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTgg < 1:559475/55‑1 (MQ=255) tAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAggcggg > 1:2024956/1‑45 (MQ=255) tAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAAtct < 1:2559445/71‑1 (MQ=255) tAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAAtc > 1:3449807/1‑70 (MQ=255) gcCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCaa < 1:3228037/71‑1 (MQ=255) gcCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCaa < 1:3155616/71‑1 (MQ=255) gcCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCaa < 1:4108864/71‑1 (MQ=255) ccATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCaaa < 1:182611/71‑1 (MQ=255) ttATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgtgcg < 1:1805428/43‑1 (MQ=255) ttATCGGCATTATTCGTTACTACACCCGTGAGGCGGgcgtgcg < 1:840665/43‑1 (MQ=255) ttATCGGCATTATTCGTTACTACACCAGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACtg > 1:1943612/1‑71 (MQ=255) tATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACtg > 1:1196987/1‑70 (MQ=255) aTCGGCATTATTCGTTACTACACCCGTGAGGCGGGcgtgcgtg > 1:1027983/1‑43 (MQ=255) aTCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACtgt < 1:662984/70‑1 (MQ=255) aTCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACtgt < 1:637412/70‑1 (MQ=255) tCGGCATTATTCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACtgtg < 1:3326083/70‑1 (MQ=255) ttattCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCa > 1:2204248/1‑69 (MQ=255) attCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAg < 1:1752294/70‑1 (MQ=255) attCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAg < 1:435235/70‑1 (MQ=255) attCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAg < 1:1730280/70‑1 (MQ=255) ttCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCg < 1:1745701/71‑1 (MQ=255) tCGTTACTGCACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCaaa > 1:1155994/1‑67 (MQ=255) cGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCaaa > 1:1937867/1‑54 (MQ=255) gTTACTGCACCCGTGATGCGGGCGTGCGTGGTCTGGAGCGTg < 1:2834008/42‑1 (MQ=38) gTTACTACACCTGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGtt < 1:464731/71‑1 (MQ=255) gTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTg < 1:2847158/42‑1 (MQ=255) tactacACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCaaa > 1:4393497/1‑51 (MQ=255) tactacACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGtt > 1:2621926/1‑69 (MQ=255) actacACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGaaa > 1:1889349/1‑42 (MQ=255) actacACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGtt > 1:2843452/1‑68 (MQ=255) tacACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGAGTCGCaaa < 1:476736/60‑1 (MQ=255) | GAACGTAATGCACTGAAAAAAGGTGAGCTGACCGTCGACGATAGCGCCATTATCGGCATTATTCGTTACTACACCCGTGAGGCGGGCGTGCGTGGTCTGGAGCGTGAAATCTCCAAACTGTGTCGCAAAGCGGTT > AP009048/459645‑459779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |