| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 704,757 | G→A | 8.5% | R131H (CGC→CAC) | nagE → | fused N‑acetyl glucosamine specific PTS enzyme IICBA components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 704,757 | 0 | G | A | 8.5% | 146.2 / 3.6 | 47 | R131H (CGC→CAC) | nagE | fused N‑acetyl glucosamine specific PTS enzyme IICBA components |
| Reads supporting (aligned to +/- strand): ref base G (20/23); new base A (2/2); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
CAGCCTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGTTACGTCTGGCC > AP009048/704694‑704823 | cAGCCTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTtgtg > 1:1675346/1‑71 (MQ=255) cTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGa < 1:435892/71‑1 (MQ=255) aaCCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGc > 1:373038/1‑71 (MQ=255) ttGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCg > 1:834101/1‑71 (MQ=255) ggTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGa > 1:3271402/1‑71 (MQ=255) tCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGcc < 1:2199239/63‑1 (MQ=255) tCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACTTTGTGCCGATTGCCACCGGAtt > 1:464031/1‑71 (MQ=255) cGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGAttct > 1:3381644/1‑71 (MQ=255) cGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGAttct > 1:2900113/1‑71 (MQ=255) gATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGAttctt < 1:4106410/71‑1 (MQ=255) gATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGAttctt > 1:4036416/1‑71 (MQ=255) atatTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGAttcttc > 1:2802358/1‑71 (MQ=255) atTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTg > 1:991750/1‑71 (MQ=255) aaaCTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCt > 1:2598891/1‑71 (MQ=255) cTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACACTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGt > 1:4311340/1‑71 (MQ=255) tGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGAttc < 1:2319023/59‑1 (MQ=255) ccGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCt > 1:4436335/1‑71 (MQ=255) cGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTg < 1:1686370/71‑1 (MQ=255) ggACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTg < 1:3787474/70‑1 (MQ=255) ggACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTg < 1:3782100/70‑1 (MQ=255) gACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGAt > 1:2927378/1‑41 (MQ=255) gACTTCCTGAGCTTCTTCGGCGGCAAACACTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTggc < 1:4130035/71‑1 (MQ=255) cTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTtgcgg > 1:2674870/1‑71 (MQ=255) ttCCTGAGCTTCTTCGGCGGCAAACACTTTGTGCCGATTGCCACCg < 1:911994/46‑1 (MQ=255) gAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCAttt > 1:2761401/1‑71 (MQ=255) aGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCAttt > 1:2902219/1‑70 (MQ=255) aGCTGCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCAttt < 1:2906072/70‑1 (MQ=255) gCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCAttttt < 1:4288030/71‑1 (MQ=255) cttcttCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTg < 1:1567666/71‑1 (MQ=255) cttcGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTgg < 1:1618341/69‑1 (MQ=255) cttcGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTgg < 1:2666487/69‑1 (MQ=255) ttcGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCGTGGTGCTGGCGGCCATTTTTgg < 1:3365516/68‑1 (MQ=255) ttcGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTggc < 1:4277252/56‑1 (MQ=255) tcGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGTTAc > 1:3123824/1‑71 (MQ=255) cggcggCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGc < 1:588419/43‑1 (MQ=255) cggcggCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGt > 1:1632643/1‑67 (MQ=255) cggcggCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGTTACg > 1:3927057/1‑71 (MQ=255) gcggcAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGt < 1:1442411/65‑1 (MQ=255) cggcAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGTTACGTCt < 1:1558918/71‑1 (MQ=255) ggcAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGTTACGTCt < 1:4158774/70‑1 (MQ=255) gcAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTggcggca < 1:3761560/53‑2 (MQ=255) gcAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTgg < 1:3393216/48‑1 (MQ=255) cAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCt < 1:1744271/39‑1 (MQ=255) aaaCGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTggcgg > 1:3348821/1‑49 (MQ=255) aaaCGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGTTACGTCTGgcc < 1:868587/71‑1 (MQ=255) cGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTgg > 1:4014980/1‑43 (MQ=255) cGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGTTACGTCt < 1:3774439/64‑1 (MQ=255) | CAGCCTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGCCGATTGCCACCGGATTCTTCTGCCTGGTGCTGGCGGCCATTTTTGGTTACGTCTGGCC > AP009048/704694‑704823 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |