| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 861,111 | G→A | 10.4% | L640L (CTG→TTG) | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 861,111 | 0 | G | A | 10.4% | 234.0 / 13.6 | 77 | L640L (CTG→TTG) | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base G (24/45); new base A (4/4); total (28/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
GACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTC > AP009048/861044‑861181 | gaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtc > 1:3768545/1‑71 (MQ=255)gaCGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTtc > 1:3195879/1‑71 (MQ=255) aCCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:390344/1‑71 (MQ=255) ccGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:3529165/71‑1 (MQ=255) ccGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata < 1:2558341/71‑1 (MQ=255) cgcgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGatat < 1:3367507/71‑1 (MQ=255) cgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAg < 1:3002994/71‑1 (MQ=255) cgTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAg > 1:1833014/1‑71 (MQ=255) tAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGt > 1:3256275/1‑71 (MQ=255) aaTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTc > 1:504897/1‑71 (MQ=255) aTAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt < 1:906763/71‑1 (MQ=255) tAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt > 1:984924/1‑70 (MQ=255) tAGTTGACGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTg < 1:2061960/71‑1 (MQ=255) gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGAtata > 1:2456791/1‑63 (MQ=255) gTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGAt < 1:3879246/71‑1 (MQ=255) ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTTGATATTGGTCTGATa < 1:1626353/71‑1 (MQ=255) ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCt < 1:1837963/67‑1 (MQ=255) ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATa < 1:1916990/71‑1 (MQ=255) ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATa < 1:1275690/71‑1 (MQ=255) ttGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGa < 1:1821212/58‑1 (MQ=255) ttccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATaa < 1:3625976/69‑1 (MQ=255) gccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATaa < 1:3046347/70‑1 (MQ=255) ccgccAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATaa < 1:2431432/69‑1 (MQ=255) ccAACCGGACCACGACTGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGAt < 1:663312/64‑1 (MQ=255) cAACGGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGt < 1:1156506/58‑1 (MQ=255) cAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGcgcg < 1:131758/70‑1 (MQ=255) cAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGa < 1:3583355/71‑1 (MQ=255) aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAg > 1:1378833/1‑71 (MQ=255) aaCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAg > 1:1193506/1‑71 (MQ=255) aCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGc > 1:2242999/1‑71 (MQ=255) aCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGc < 1:816599/71‑1 (MQ=255) aCCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTAGTCGATATAGGTCTGATAAGCGCGAGc > 1:3211280/1‑71 (MQ=255) cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:1916978/47‑1 (MQ=255) cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGc > 1:1416510/1‑69 (MQ=255) cGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCca < 1:2601823/71‑1 (MQ=255) ggACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcag < 1:2666780/71‑1 (MQ=255) gACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGt < 1:95220/38‑1 (MQ=255) gACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCcag > 1:1137198/1‑70 (MQ=255) aCCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa < 1:2882320/44‑1 (MQ=255) ccACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata > 1:4384140/1‑45 (MQ=255) ccACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata > 1:1857583/1‑45 (MQ=255) ccACGACCGTAGCGCGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCcagcag > 1:1723073/1‑71 (MQ=255) aCGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCg < 1:4167001/71‑1 (MQ=255) cGATCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGa > 1:2764122/1‑40 (MQ=255) cGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCATCAGCGt < 1:4187210/71‑1 (MQ=255) cGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt < 1:3844294/71‑1 (MQ=255) cGACAGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGt < 1:315362/71‑1 (MQ=255) gACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTa > 1:3409938/1‑71 (MQ=255) aCCGTAGCGCGGATTAT‑GTACTGTTTCAGTTCGTCGATATAGGTCTGATAAgcgct < 1:1708916/56‑2 (MQ=255) ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGata > 1:4215939/1‑39 (MQ=255) ccGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATc < 1:1400007/71‑1 (MQ=255) gTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGa < 1:3347149/71‑1 (MQ=255) gcgcGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACa < 1:2042956/70‑1 (MQ=255) gcgcGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAg > 1:4481664/1‑71 (MQ=255) gcgcGGATTATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACa < 1:2242564/70‑1 (MQ=255) ggATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTat > 1:381740/1‑70 (MQ=255) gATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTatca < 1:771253/71‑1 (MQ=255) aTTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTatcat < 1:3040909/71‑1 (MQ=255) aTTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTatcat < 1:276061/71‑1 (MQ=255) ttATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTatcatc > 1:3982577/1‑71 (MQ=255) ttATGGTACTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTatcatc < 1:3620446/71‑1 (MQ=255) tGGTACTGTTTCAGTTCGTCGATATAGGTCTGCTAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcgtc < 1:1960569/71‑1 (MQ=255) tGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcgt < 1:447600/70‑1 (MQ=255) gTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCAtcgtcg < 1:116310/70‑1 (MQ=255) tACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGa < 1:268216/39‑1 (MQ=255) tACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTa < 1:161664/60‑1 (MQ=255) tACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGtt < 1:2774723/71‑1 (MQ=255) tACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCCTATCGACAGTa < 1:4532778/60‑1 (MQ=255) tACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGAGAGCcagca < 1:3503057/46‑1 (MQ=255) cTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGc < 1:4238413/71‑1 (MQ=255) cTGTTTCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGc > 1:3597478/1‑71 (MQ=255) gTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCg > 1:4454455/1‑71 (MQ=255) tttCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGcc < 1:1339209/69‑1 (MQ=255) tCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACa > 1:2412600/1‑50 (MQ=255) tCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTAc > 1:4392299/1‑71 (MQ=255) tCAATTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTAc > 1:2381007/1‑71 (MQ=255) aGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACtt < 1:1373143/71‑1 (MQ=255) gTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTc < 1:2921677/71‑1 (MQ=255) | GACGTACCCGCGTAATAGTTGCCGCCAACCGGACCACGACCGTAGCGCGGATTATGGTACTGTTTCAGTTCGTCGATATAGGTCTGATAAGCGCGAGCCAGCAGCGTATCGACAGTATCATCGTCGTTGCCGTACTTC > AP009048/861044‑861181 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |