| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 1,008,134 | C→T | 14.3% | intergenic (‑112/‑132) | ycbX ← / → ycbY | predicted 2Fe‑2S cluster‑containing protein/predicted methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 1,008,134 | 0 | C | T | 14.3% | 130.9 / 12.2 | 49 | intergenic (‑112/‑132) | ycbX/ycbY | predicted 2Fe‑2S cluster‑containing protein/predicted methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (24/18); new base T (4/3); total (28/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01 | |||||||||||
ATGACGCGCTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCCGCG > AP009048/1008064‑1008196 | atgacGCGCTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTc < 1:3958621/71‑1 (MQ=255) tgacGCGCTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCa < 1:1686882/71‑1 (MQ=255) acGCGCTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGt < 1:744039/71‑1 (MQ=255) gcTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGttt > 1:2382601/1‑69 (MQ=255) gcTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTgg > 1:2834007/1‑71 (MQ=255) attaGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGccc < 1:2824807/70‑1 (MQ=255) ttaGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCt < 1:1971466/70‑1 (MQ=255) aGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTggg > 1:2052843/1‑71 (MQ=255) gCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAg < 1:2564096/58‑1 (MQ=255) taATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGt < 1:730793/55‑1 (MQ=255) cgcAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTa > 1:1034021/1‑64 (MQ=255) caacaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGt < 1:3158675/60‑1 (MQ=255) aacaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGaaa < 1:2545960/70‑1 (MQ=255) acaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGtt > 1:1503744/1‑47 (MQ=255) acaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTg < 1:1862124/49‑1 (MQ=255) acaaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTc > 1:3142928/1‑65 (MQ=255) caaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATcc > 1:3829435/1‑71 (MQ=255) caaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATcc > 1:869175/1‑71 (MQ=255) aaTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCt < 1:1977395/71‑1 (MQ=255) aTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTc < 1:2875169/71‑1 (MQ=255) aTTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCt > 1:2119541/1‑70 (MQ=255) ttttCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCa > 1:3935212/1‑71 (MQ=255) ttAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGa < 1:3911432/58‑1 (MQ=255) aGCGCCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTa > 1:3215538/1‑49 (MQ=255) gcgcCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACtt < 1:3391616/70‑1 (MQ=255) gcgcCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACtt < 1:480070/70‑1 (MQ=255) cgcCTGTCCCATTAGGCTATTTTATTTGTTAGTTTGGcc > 1:2437247/1‑39 (MQ=255) cgcCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCt > 1:3564239/1‑51 (MQ=255) ccTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGa > 1:2911969/1‑52 (MQ=255) ccTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTgtgt < 1:4285775/71‑1 (MQ=255) cTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCt < 1:329896/57‑1 (MQ=255) cTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTa > 1:4281646/1‑71 (MQ=255) tGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTAc > 1:2459482/1‑71 (MQ=255) tGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTAc > 1:1984108/1‑71 (MQ=255) tCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGa > 1:1492523/1‑48 (MQ=255) tCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGa > 1:1979089/1‑48 (MQ=255) tCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACg < 1:3518310/70‑1 (MQ=255) cccATTAGGCTATTTTATTTGTCAGTTTGGCCCTg > 1:2264262/1‑35 (MQ=255) ccATTAGGCTATTTTTTTTGTCAGTTTGGCCCTGGGTTATGCTCGaaa > 1:715284/1‑48 (MQ=255) ccATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGaaa > 1:2585565/1‑48 (MQ=255) aTTAGGCTATTTTATTTGTTAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTgtgt > 1:4308692/1‑63 (MQ=255) aTTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGaaa < 1:4175231/46‑1 (MQ=255) aGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCa > 1:262286/1‑49 (MQ=255) cTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTtct < 1:4551545/71‑1 (MQ=255) attttatttGTCAGTTTGGCCCTGGGTTATGCTCGaaa < 1:3127992/38‑1 (MQ=255) ttttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCcg > 1:2910172/1‑71 (MQ=255) tttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTAc < 1:277807/47‑1 (MQ=255) tttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCg > 1:1062503/1‑61 (MQ=255) tttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCcgc > 1:2417405/1‑71 (MQ=255) ttatttGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCcgcg > 1:1826602/1‑71 (MQ=255) | ATGACGCGCTTATTAGCTATAATGCGCAACAATTTTCTTAGCGCCTGTCCCATTAGGCTATTTTATTTGTCAGTTTGGCCCTGGGTTATGCTCGAAATCCTCACGTACTTGTGTACGCTCCGGTTTCTCCGCG > AP009048/1008064‑1008196 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |